In vivo nanoparticle-based T cell imaging can predict therapy response towards adoptive T cell therapy in experimental glioma
Rationale: Intrinsic brain tumors, such as gliomas are largely resistant to immunotherapies including immune checkpoint blockade. Adoptive cell therapies (ACT) including chimeric antigen receptor (CAR) or T cell receptor (TCR)-transgenic T cell therapy targeting glioma-associated antigens are an eme...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
2023.09.25
|
| In: |
Theranostics
Year: 2023, Volume: 13, Issue: 15, Pages: 5170-5182 |
| ISSN: | 1838-7640 |
| DOI: | 10.7150/thno.87248 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.7150/thno.87248 Verlag, lizenzpflichtig, Volltext: https://www.thno.org/v13p5170.htm 
|
| Author Notes: | Jessica Hunger, Katharina Schregel, Berin Boztepe, Dennis Alexander Agardy, Verena Turco, Kianush Karimian-Jazi, Ina Weidenfeld, Yannik Streibel, Manuel Fischer, Volker Sturm, Rachel Santarella-Mellwig, Michael Kilian, Kristine Jähne, Katharina Sahm, Wolfgang Wick, Lukas Bunse, Sabine Heiland, Theresa Bunse, Martin Bendszus, Michael Platten and Michael O. Breckwoldt |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1891935321 | ||
| 003 | DE-627 | ||
| 005 | 20241205143353.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240620s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.7150/thno.87248 |2 doi | |
| 035 | |a (DE-627)1891935321 | ||
| 035 | |a (DE-599)KXP1891935321 | ||
| 035 | |a (OCoLC)1475300247 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Hunger, Jessica |d 1996- |e VerfasserIn |0 (DE-588)1295759705 |0 (DE-627)1852388692 |4 aut | |
| 245 | 1 | 0 | |a In vivo nanoparticle-based T cell imaging can predict therapy response towards adoptive T cell therapy in experimental glioma |c Jessica Hunger, Katharina Schregel, Berin Boztepe, Dennis Alexander Agardy, Verena Turco, Kianush Karimian-Jazi, Ina Weidenfeld, Yannik Streibel, Manuel Fischer, Volker Sturm, Rachel Santarella-Mellwig, Michael Kilian, Kristine Jähne, Katharina Sahm, Wolfgang Wick, Lukas Bunse, Sabine Heiland, Theresa Bunse, Martin Bendszus, Michael Platten and Michael O. Breckwoldt |
| 264 | 1 | |c 2023.09.25 | |
| 300 | |b Illustrationen | ||
| 300 | |a 13 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 20.06.2024 | ||
| 520 | |a Rationale: Intrinsic brain tumors, such as gliomas are largely resistant to immunotherapies including immune checkpoint blockade. Adoptive cell therapies (ACT) including chimeric antigen receptor (CAR) or T cell receptor (TCR)-transgenic T cell therapy targeting glioma-associated antigens are an emerging field in glioma immunotherapy. However, imaging techniques for non-invasive monitoring of adoptively transferred T cells homing to the glioma microenvironment are currently lacking. Methods: Ultrasmall iron oxide nanoparticles (NP) can be visualized non-invasively by magnetic resonance imaging (MRI) and dedicated MRI sequences such as T2* mapping. Here, we develop a protocol for efficient ex vivo labeling of murine and human TCR-transgenic and CAR T cells with iron oxide NPs. We assess labeling efficiency and T cell functionality by flow cytometry and transmission electron microscopy (TEM). NP labeled T cells are visualized by MRI at 9.4 T in vivo after adoptive T cell transfer and correlated with 3D models of cleared brains obtained by light sheet microscopy (LSM). Results: NP are incorporated into T cells in subcellular cytoplasmic vesicles with high labeling efficiency without interfering with T cell viability, proliferation and effector function as assessed by cytokine secretion and antigen-specific killing assays in vitro. We further demonstrate that adoptively transferred T cells can be longitudinally monitored intratumorally by high field MRI at 9.4 Tesla in a murine glioma model with high sensitivity. We find that T cell influx and homogenous spatial distribution of T cells within the TME as assessed by T2* imaging predicts tumor response to ACT whereas incomplete T cell coverage results in treatment resistance. Conclusion: This study showcases a rational for monitoring adoptive T cell therapies non-invasively by iron oxide NP in gliomas to track intratumoral T cell influx and ultimately predict treatment outcome. | ||
| 700 | 1 | |a Schregel, Katharina |e VerfasserIn |0 (DE-588)1215056605 |0 (DE-627)1726087360 |4 aut | |
| 700 | 1 | |a Boztepe, Berin |d 1996- |e VerfasserIn |0 (DE-588)1283251558 |0 (DE-627)1839055898 |4 aut | |
| 700 | 1 | |a Agardy, Dennis |d 1993- |e VerfasserIn |0 (DE-588)1309845611 |0 (DE-627)1870235274 |4 aut | |
| 700 | 1 | |a Turco, Verena |e VerfasserIn |0 (DE-588)1304248763 |0 (DE-627)1860413714 |4 aut | |
| 700 | 1 | |a Karimian-Jazi, Kianush |e VerfasserIn |0 (DE-588)1074833155 |0 (DE-627)832569186 |0 (DE-576)442980353 |4 aut | |
| 700 | 1 | |a Weidenfeld, Ina |e VerfasserIn |0 (DE-588)139001662 |0 (DE-627)703177176 |0 (DE-576)309334217 |4 aut | |
| 700 | 1 | |a Streibel, Yannik |e VerfasserIn |0 (DE-588)1304249360 |0 (DE-627)1860414524 |4 aut | |
| 700 | 1 | |a Fischer, Manuel |e VerfasserIn |0 (DE-588)1155954491 |0 (DE-627)1018513051 |0 (DE-576)501959688 |4 aut | |
| 700 | 1 | |a Sturm, Volker Jörg Friedrich |d 1980- |e VerfasserIn |0 (DE-588)1096228149 |0 (DE-627)856637238 |0 (DE-576)467929971 |4 aut | |
| 700 | 1 | |a Santarella-Mellwig, Rachel |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kilian, Michael |d 1990- |e VerfasserIn |0 (DE-588)1220319422 |0 (DE-627)1736693255 |4 aut | |
| 700 | 1 | |a Jähne, Kristine |e VerfasserIn |4 aut | |
| 700 | 1 | |a Sahm, Katharina |d 1985- |e VerfasserIn |0 (DE-588)1048541762 |0 (DE-627)780594371 |0 (DE-576)402374452 |4 aut | |
| 700 | 1 | |a Wick, Wolfgang |d 1970- |e VerfasserIn |0 (DE-588)120297736 |0 (DE-627)080586929 |0 (DE-576)186221320 |4 aut | |
| 700 | 1 | |a Bunse, Lukas |d 1988- |e VerfasserIn |0 (DE-588)1051941229 |0 (DE-627)787187399 |0 (DE-576)407600426 |4 aut | |
| 700 | 1 | |a Heiland, Sabine |e VerfasserIn |0 (DE-588)106732626X |0 (DE-627)818624450 |0 (DE-576)426561368 |4 aut | |
| 700 | 1 | |a Bunse, Theresa |d 1985- |e VerfasserIn |0 (DE-588)105282112X |0 (DE-627)789079070 |0 (DE-576)408553731 |4 aut | |
| 700 | 1 | |a Bendszus, Martin |e VerfasserIn |0 (DE-588)1032676426 |0 (DE-627)738634131 |0 (DE-576)175567697 |4 aut | |
| 700 | 1 | |a Platten, Michael |d 1971- |e VerfasserIn |0 (DE-588)121627780 |0 (DE-627)081431848 |0 (DE-576)261969811 |4 aut | |
| 700 | 1 | |a Breckwoldt, Michael O. |d 1983- |e VerfasserIn |0 (DE-588)1022496298 |0 (DE-627)716993392 |0 (DE-576)365498297 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Theranostics |d Wyoming, NSW : Ivyspring, 2011 |g 13(2023), 15, Seite 5170-5182 |h Online-Ressource |w (DE-627)645091030 |w (DE-600)2592097-2 |w (DE-576)336963033 |x 1838-7640 |7 nnas |a In vivo nanoparticle-based T cell imaging can predict therapy response towards adoptive T cell therapy in experimental glioma |
| 773 | 1 | 8 | |g volume:13 |g year:2023 |g number:15 |g pages:5170-5182 |g extent:13 |a In vivo nanoparticle-based T cell imaging can predict therapy response towards adoptive T cell therapy in experimental glioma |
| 856 | 4 | 0 | |u https://doi.org/10.7150/thno.87248 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.thno.org/v13p5170.htm |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20240620 | ||
| 992 | |a 20240620 | ||
| 993 | |a Article | ||
| 993 | |a Article | ||
| 994 | |a 2023 | ||
| 994 | |a 2023 | ||
| 998 | |g 1022496298 |a Breckwoldt, Michael O. |m 1022496298:Breckwoldt, Michael O. |d 910000 |d 911100 |e 910000PB1022496298 |e 911100PB1022496298 |k 0/910000/ |k 1/910000/911100/ |p 21 |y j | ||
| 998 | |g 121627780 |a Platten, Michael |m 121627780:Platten, Michael |d 60000 |e 60000PP121627780 |k 0/60000/ |p 20 | ||
| 998 | |g 1032676426 |a Bendszus, Martin |m 1032676426:Bendszus, Martin |d 910000 |d 911100 |e 910000PB1032676426 |e 911100PB1032676426 |k 0/910000/ |k 1/910000/911100/ |p 19 | ||
| 998 | |g 121627780 |a Platten, Michael |m 121627780:Platten, Michael |d 60000 |d 62700 |e 60000PP121627780 |e 62700PP121627780 |k 0/60000/ |k 1/60000/62700/ |p 20 | ||
| 998 | |g 105282112X |a Bunse, Theresa |m 105282112X:Bunse, Theresa |d 60000 |d 62700 |e 60000PB105282112X |e 62700PB105282112X |k 0/60000/ |k 1/60000/62700/ |p 18 | ||
| 998 | |g 106732626X |a Heiland, Sabine |m 106732626X:Heiland, Sabine |d 910000 |d 911100 |e 910000PH106732626X |e 911100PH106732626X |k 0/910000/ |k 1/910000/911100/ |p 17 | ||
| 998 | |g 1051941229 |a Bunse, Lukas |m 1051941229:Bunse, Lukas |d 60000 |d 62700 |e 60000PB1051941229 |e 62700PB1051941229 |k 0/60000/ |k 1/60000/62700/ |p 16 | ||
| 998 | |g 120297736 |a Wick, Wolfgang |m 120297736:Wick, Wolfgang |d 910000 |d 911100 |e 910000PW120297736 |e 911100PW120297736 |k 0/910000/ |k 1/910000/911100/ |p 15 | ||
| 998 | |g 1048541762 |a Sahm, Katharina |m 1048541762:Sahm, Katharina |d 60000 |d 62700 |e 60000PS1048541762 |e 62700PS1048541762 |k 0/60000/ |k 1/60000/62700/ |p 14 | ||
| 998 | |g 1220319422 |a Kilian, Michael |m 1220319422:Kilian, Michael |d 60000 |d 62700 |e 60000PK1220319422 |e 62700PK1220319422 |k 0/60000/ |k 1/60000/62700/ |p 12 | ||
| 998 | |g 1096228149 |a Sturm, Volker Jörg Friedrich |m 1096228149:Sturm, Volker Jörg Friedrich |d 910000 |d 911100 |e 910000PS1096228149 |e 911100PS1096228149 |k 0/910000/ |k 1/910000/911100/ |p 10 | ||
| 998 | |g 1155954491 |a Fischer, Manuel |m 1155954491:Fischer, Manuel |d 50000 |e 50000PF1155954491 |k 0/50000/ |p 9 | ||
| 998 | |g 1304249360 |a Streibel, Yannik |m 1304249360:Streibel, Yannik |d 50000 |e 50000PS1304249360 |k 0/50000/ |p 8 | ||
| 998 | |g 139001662 |a Weidenfeld, Ina |m 139001662:Weidenfeld, Ina |d 910000 |d 911100 |e 910000PW139001662 |e 911100PW139001662 |k 0/910000/ |k 1/910000/911100/ |p 7 | ||
| 998 | |g 1074833155 |a Karimian-Jazi, Kianush |m 1074833155:Karimian-Jazi, Kianush |d 910000 |d 911100 |e 910000PK1074833155 |e 911100PK1074833155 |k 0/910000/ |k 1/910000/911100/ |p 6 | ||
| 998 | |g 1304248763 |a Turco, Verena |m 1304248763:Turco, Verena |d 910000 |d 910100 |e 910000PT1304248763 |e 910100PT1304248763 |k 0/910000/ |k 1/910000/910100/ |p 5 | ||
| 998 | |g 1309845611 |a Agardy, Dennis |m 1309845611:Agardy, Dennis |d 60000 |d 62700 |e 60000PA1309845611 |e 62700PA1309845611 |k 0/60000/ |k 1/60000/62700/ |p 4 | ||
| 998 | |g 1283251558 |a Boztepe, Berin |m 1283251558:Boztepe, Berin |d 910000 |d 911100 |e 910000PB1283251558 |e 911100PB1283251558 |k 0/910000/ |k 1/910000/911100/ |p 3 | ||
| 998 | |g 1215056605 |a Schregel, Katharina |m 1215056605:Schregel, Katharina |d 910000 |d 911100 |e 910000PS1215056605 |e 911100PS1215056605 |k 0/910000/ |k 1/910000/911100/ |p 2 | ||
| 998 | |g 1295759705 |a Hunger, Jessica |m 1295759705:Hunger, Jessica |d 910000 |d 911100 |e 910000PH1295759705 |e 911100PH1295759705 |k 0/910000/ |k 1/910000/911100/ |p 1 |x j | ||
| 999 | |a KXP-PPN1891935321 |e 4540712934 | ||
| 999 | |a KXP-PPN1891935321 |e 4540700979 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"type":{"bibl":"periodical","media":"Online-Ressource"},"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"id":{"zdb":["2592097-2"],"issn":["1838-7640"],"eki":["645091030"]},"disp":"In vivo nanoparticle-based T cell imaging can predict therapy response towards adoptive T cell therapy in experimental gliomaTheranostics","note":["Gesehen am 11.11.20"],"title":[{"title":"Theranostics","title_sort":"Theranostics"}],"pubHistory":["1.2011 -"],"part":{"extent":"13","volume":"13","pages":"5170-5182","year":"2023","issue":"15","text":"13(2023), 15, Seite 5170-5182"},"recId":"645091030","origin":[{"publisher":"Ivyspring","dateIssuedKey":"2011","dateIssuedDisp":"2011-","publisherPlace":"Wyoming, NSW"}]}],"name":{"displayForm":["Jessica Hunger, Katharina Schregel, Berin Boztepe, Dennis Alexander Agardy, Verena Turco, Kianush Karimian-Jazi, Ina Weidenfeld, Yannik Streibel, Manuel Fischer, Volker Sturm, Rachel Santarella-Mellwig, Michael Kilian, Kristine Jähne, Katharina Sahm, Wolfgang Wick, Lukas Bunse, Sabine Heiland, Theresa Bunse, Martin Bendszus, Michael Platten and Michael O. Breckwoldt"]},"language":["eng"],"person":[{"family":"Hunger","role":"aut","display":"Hunger, Jessica","given":"Jessica"},{"family":"Schregel","display":"Schregel, Katharina","role":"aut","given":"Katharina"},{"family":"Boztepe","given":"Berin","role":"aut","display":"Boztepe, Berin"},{"family":"Agardy","given":"Dennis","display":"Agardy, Dennis","role":"aut"},{"family":"Turco","display":"Turco, Verena","role":"aut","given":"Verena"},{"family":"Karimian-Jazi","given":"Kianush","display":"Karimian-Jazi, Kianush","role":"aut"},{"given":"Ina","role":"aut","display":"Weidenfeld, Ina","family":"Weidenfeld"},{"given":"Yannik","display":"Streibel, Yannik","role":"aut","family":"Streibel"},{"display":"Fischer, Manuel","role":"aut","given":"Manuel","family":"Fischer"},{"family":"Sturm","given":"Volker Jörg Friedrich","role":"aut","display":"Sturm, Volker Jörg Friedrich"},{"family":"Santarella-Mellwig","role":"aut","display":"Santarella-Mellwig, Rachel","given":"Rachel"},{"family":"Kilian","role":"aut","display":"Kilian, Michael","given":"Michael"},{"given":"Kristine","display":"Jähne, Kristine","role":"aut","family":"Jähne"},{"display":"Sahm, Katharina","role":"aut","given":"Katharina","family":"Sahm"},{"family":"Wick","given":"Wolfgang","display":"Wick, Wolfgang","role":"aut"},{"role":"aut","display":"Bunse, Lukas","given":"Lukas","family":"Bunse"},{"family":"Heiland","given":"Sabine","display":"Heiland, Sabine","role":"aut"},{"role":"aut","display":"Bunse, Theresa","given":"Theresa","family":"Bunse"},{"given":"Martin","role":"aut","display":"Bendszus, Martin","family":"Bendszus"},{"given":"Michael","display":"Platten, Michael","role":"aut","family":"Platten"},{"role":"aut","display":"Breckwoldt, Michael O.","given":"Michael O.","family":"Breckwoldt"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"physDesc":[{"noteIll":"Illustrationen","extent":"13 S."}],"id":{"eki":["1891935321"],"doi":["10.7150/thno.87248"]},"note":["Gesehen am 20.06.2024"],"title":[{"title":"In vivo nanoparticle-based T cell imaging can predict therapy response towards adoptive T cell therapy in experimental glioma","title_sort":"In vivo nanoparticle-based T cell imaging can predict therapy response towards adoptive T cell therapy in experimental glioma"}],"origin":[{"dateIssuedKey":"2023","dateIssuedDisp":"2023.09.25"}],"recId":"1891935321"} | ||
| SRT | |a HUNGERJESSINVIVONANO2023 | ||