Modification of cell surfaces by enzymatic introduction of special sialic acid analogues
Taking advantage of the defined acceptor specifity of rat liver α2,6-sialyltransferase, N-linked Ga1β1,4GlcNAc-sequences of glycoconjugates at the plasma membrane of viable human erythrorytes and lymphoblastic IN-9 cells were modified to terminate in three NeuAc analogues endowed with special bemche...
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| Hauptverfasser: | , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
15 February 1993
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| In: |
Biochemical and biophysical research communications
Year: 1993, Jahrgang: 190, Heft: 3, Pages: 914-920 |
| ISSN: | 1090-2104 |
| DOI: | 10.1006/bbrc.1993.1136 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1006/bbrc.1993.1136 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0006291X83711368 |
| Verfasserangaben: | Renate E. Kosa, Reinhard Brossmer, Hans Jürgen Groß |
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| 520 | |a Taking advantage of the defined acceptor specifity of rat liver α2,6-sialyltransferase, N-linked Ga1β1,4GlcNAc-sequences of glycoconjugates at the plasma membrane of viable human erythrorytes and lymphoblastic IN-9 cells were modified to terminate in three NeuAc analogues endowed with special bemchemical properties: sialidase-resistant 9-amino-NeuAc, O-acetylesterase-resistant 9-acetamido-NeuAc and photolabile 9-azido-NeuAc. Incorporation of the latter analogues and of parent NeuAc proceeded to similar values, whereas 9-amino-NeuAc yielded slightly reduced values. | ||
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