Chemo- and optogenetic activation of hypothalamic Foxb1-expressing neurons and their terminal endings in the rostral-dorsolateral PAG leads to tachypnea, bradycardia, and immobility
Foxb1 -expressing neurons occur in the dorsal premammillary nucleus (PMd) and further rostrally in the parvafox nucleus, a longitudinal cluster of neurons in the lateral hypothalamus of rodents. The descending projection of these Foxb1+ neurons end in the dorsolateral part of the periaqueductal gray...
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| Hauptverfasser: | , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
01 February, 2024
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| In: |
eLife
Year: 2024, Jahrgang: 12, Pages: 1-26 |
| ISSN: | 2050-084X |
| DOI: | 10.7554/eLife.86737 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.7554/eLife.86737
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| Verfasserangaben: | Reto B. Cola, Diana M. Roccaro-Waldmeyer, Samara Naim, Alexandre Babalian, Petra Seebeck, Gonzalo Alvarez-Bolado, Marco R. Celio |
MARC
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| 245 | 1 | 0 | |a Chemo- and optogenetic activation of hypothalamic Foxb1-expressing neurons and their terminal endings in the rostral-dorsolateral PAG leads to tachypnea, bradycardia, and immobility |c Reto B. Cola, Diana M. Roccaro-Waldmeyer, Samara Naim, Alexandre Babalian, Petra Seebeck, Gonzalo Alvarez-Bolado, Marco R. Celio |
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| 520 | |a Foxb1 -expressing neurons occur in the dorsal premammillary nucleus (PMd) and further rostrally in the parvafox nucleus, a longitudinal cluster of neurons in the lateral hypothalamus of rodents. The descending projection of these Foxb1+ neurons end in the dorsolateral part of the periaqueductal gray (dlPAG). The functional role of the Foxb1+ neuronal subpopulation in the PMd and the parvafox nucleus remains elusive. In this study, the activity of the Foxb1+ neurons and of their terminal endings in the dlPAG in mice was selectively altered by employing chemo- and optogenetic tools. Our results show that in whole-body barometric plethysmography, hM3Dq-mediated, global Foxb1+ neuron excitation activates respiration. Time-resolved optogenetic gain-of-function manipulation of the terminal endings of Foxb1+ neurons in the rostral third of the dlPAG leads to abrupt immobility and bradycardia. Chemogenetic activation of Foxb1+ cell bodies and ChR2-mediated excitation of their axonal endings in the dlPAG led to a phenotypical presentation congruent with a 'freezing-like' situation during innate defensive behavior. | ||
| 650 | 4 | |a Animals | |
| 650 | 4 | |a Bradycardia | |
| 650 | 4 | |a defensive behavior | |
| 650 | 4 | |a dorsal premammillary nucleus | |
| 650 | 4 | |a Forkhead Transcription Factors | |
| 650 | 4 | |a Hypothalamus | |
| 650 | 4 | |a immobility | |
| 650 | 4 | |a Mice | |
| 650 | 4 | |a mouse | |
| 650 | 4 | |a Neurons | |
| 650 | 4 | |a neuroscience | |
| 650 | 4 | |a Optogenetics | |
| 650 | 4 | |a parvafox nucleus | |
| 650 | 4 | |a periaqueductal gray | |
| 650 | 4 | |a respiration | |
| 650 | 4 | |a Tachypnea | |
| 700 | 1 | |a Roccaro-Waldmeyer, Diana M. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Naim, Samara |e VerfasserIn |4 aut | |
| 700 | 1 | |a Babalian, Alexandre |e VerfasserIn |4 aut | |
| 700 | 1 | |a Seebeck, Petra |e VerfasserIn |4 aut | |
| 700 | 1 | |a Alvarez-Bolado, Gonzalo |e VerfasserIn |0 (DE-588)1072657805 |0 (DE-627)827961847 |0 (DE-576)177489537 |4 aut | |
| 700 | 1 | |a Celio, Marco R. |e VerfasserIn |4 aut | |
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