Buchumschlag

Caveolae disassemble upon membrane lesioning and foster cell survival

Repair of lesions in the plasma membrane is key to sustaining cellular homeostasis. Cells maintain cytoplasmic as well as membrane-bound stores of repair proteins that can rapidly precipitate at the site of membrane lesions. However, little is known about the origins of lipids and proteins for resea...

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Hauptverfasser: Štefl, Martin (VerfasserIn) , Takamiya, Masanari (VerfasserIn) , Middel, Volker (VerfasserIn) , Tekpınar, Miyase (VerfasserIn) , Nienhaus, Karin (VerfasserIn) , Beil, Tanja (VerfasserIn) , Rastegar, Sepand (VerfasserIn) , Strähle, Uwe (VerfasserIn) , Nienhaus, Gerd Ulrich (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 9 January 2024
In: iScience
Year: 2024, Jahrgang: 27, Heft: 2, Pages: 1-20
ISSN:2589-0042
DOI:10.1016/j.isci.2024.108849
Online-Zugang:Verlag, kostenfrei, Volltext: https://doi.org/10.1016/j.isci.2024.108849
Volltext
Verfasserangaben:Martin Štefl, Masanari Takamiya, Volker Middel, Miyase Tekpınar, Karin Nienhaus, Tanja Beil, Sepand Rastegar, Uwe Strähle, Gerd Ulrich Nienhaus
Beschreibung
Zusammenfassung:Repair of lesions in the plasma membrane is key to sustaining cellular homeostasis. Cells maintain cytoplasmic as well as membrane-bound stores of repair proteins that can rapidly precipitate at the site of membrane lesions. However, little is known about the origins of lipids and proteins for resealing and repair of the plasma membrane. Here we study the dynamics of caveolar proteins after laser-induced lesioning of plasma membranes of mammalian C2C12 tissue culture cells and muscle cells of intact zebrafish embryos. Single-molecule diffusivity measurements indicate that caveolar clusters break up into smaller entities after wounding. Unlike Annexins and Dysferlin, caveolar proteins do not accumulate at the lesion patch. In caveolae-depleted cavin1a knockout zebrafish embryos, lesion patch formation is impaired, and injured cells show reduced survival. Our data suggest that caveolae disassembly releases surplus plasma membrane near the lesion to facilitate membrane repair after initial patch formation for emergency sealing.
Beschreibung:Gesehen am 26.07.2024
Beschreibung:Online Resource
ISSN:2589-0042
DOI:10.1016/j.isci.2024.108849

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