Hepatic passaging of NRAS-mutant melanoma influences adhesive properties and metastatic pattern
Liver metastasis is a poor prognostic factor for treatment of advanced cutaneous melanoma with either immunotherapy or targeted therapies. In this study we focused on NRAS mutated melanoma, a cohort with high unmet clinical need. WT31 melanoma was repeatedly passaged over the liver after intravenous...
Gespeichert in:
| Hauptverfasser: | , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
13 May 2023
|
| In: |
BMC cancer
Year: 2023, Jahrgang: 23, Heft: 1, Pages: 1-16 |
| ISSN: | 1471-2407 |
| DOI: | 10.1186/s12885-023-10912-4 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1186/s12885-023-10912-4 Verlag, kostenfrei, Volltext: https://bmccancer.biomedcentral.com/articles/10.1186/s12885-023-10912-4 
|
| Verfasserangaben: | Bianca Dietsch, Céline Weller, Carsten Sticht, Carolina de la Torre, Martin Kramer, Sergij Goerdt, Cyrill Géraud and Sebastian A. Wohlfeil |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1898058423 | ||
| 003 | DE-627 | ||
| 005 | 20241205161129.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240807s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1186/s12885-023-10912-4 |2 doi | |
| 035 | |a (DE-627)1898058423 | ||
| 035 | |a (DE-599)KXP1898058423 | ||
| 035 | |a (OCoLC)1475306404 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Dietsch, Bianca |d 1986- |e VerfasserIn |0 (DE-588)1251327389 |0 (DE-627)1788807359 |4 aut | |
| 245 | 1 | 0 | |a Hepatic passaging of NRAS-mutant melanoma influences adhesive properties and metastatic pattern |c Bianca Dietsch, Céline Weller, Carsten Sticht, Carolina de la Torre, Martin Kramer, Sergij Goerdt, Cyrill Géraud and Sebastian A. Wohlfeil |
| 264 | 1 | |c 13 May 2023 | |
| 300 | |b Illustrationen | ||
| 300 | |a 16 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 07.08.2024 | ||
| 520 | |a Liver metastasis is a poor prognostic factor for treatment of advanced cutaneous melanoma with either immunotherapy or targeted therapies. In this study we focused on NRAS mutated melanoma, a cohort with high unmet clinical need. WT31 melanoma was repeatedly passaged over the liver after intravenous injections five times generating the subline WT31_P5IV. The colonization of target organs, morphology, vascularization and the gene expression profiles of metastases were analyzed. After intravenous injection lung metastasis was significantly decreased and a trend towards increased liver metastasis was detected for WT31_P5IV as compared to parental WT31. Besides, the ratio of lung to liver metastases was significantly smaller. Histology of lung metastases revealed reduced proliferation of WT31_P5IV in relation to WT31 while both size and necrotic areas were unaltered. Liver metastases of both sublines showed no differences in vascularization, proliferation or necrosis. To identify tumor-intrinsic factors that altered the metastatic pattern of WT31_P5IV RNA sequencing was performed and revealed a differential regulation of pathways involved in cell adhesion. Ex vivo fluorescence imaging confirmed that initial tumor cell retention in the lungs was significantly reduced in WT31_P5IV in comparison to WT31. This study demonstrates that tumor-intrinsic properties influencing the metastatic pattern of NRAS mutated melanoma are strongly affected by hepatic passaging and the hematogenous route tumor cells take. It has implications for the clinical setting as such effects might also occur during metastatic spread or disease progression in melanoma patients. | ||
| 700 | 1 | |a Weller, Céline |d 1993- |e VerfasserIn |0 (DE-588)1251329381 |0 (DE-627)1788846249 |4 aut | |
| 700 | 1 | |a Sticht, Carsten |d 1972- |e VerfasserIn |0 (DE-588)1020115718 |0 (DE-627)691110077 |0 (DE-576)250042177 |4 aut | |
| 700 | 1 | |a Torre, Carolina de la |d 1985- |e VerfasserIn |0 (DE-588)1122355831 |0 (DE-627)875651283 |0 (DE-576)481254137 |4 aut | |
| 700 | 1 | |a Kramer, Martin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Goerdt, Sergij |d 1959- |e VerfasserIn |0 (DE-588)1027602177 |0 (DE-627)729460010 |0 (DE-576)373451512 |4 aut | |
| 700 | 1 | |a Géraud, Cyrill |d 1982- |e VerfasserIn |0 (DE-588)137867808 |0 (DE-627)696132583 |0 (DE-576)304908932 |4 aut | |
| 700 | 1 | |a Wohlfeil, Sebastian A. |e VerfasserIn |0 (DE-588)1109821379 |0 (DE-627)864416334 |0 (DE-576)475626575 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t BMC cancer |d London : BioMed Central, 2001 |g 23(2023), 1, Seite 1-16 |h Online-Ressource |w (DE-627)326643710 |w (DE-600)2041352-X |w (DE-576)107014645 |x 1471-2407 |7 nnas |a Hepatic passaging of NRAS-mutant melanoma influences adhesive properties and metastatic pattern |
| 773 | 1 | 8 | |g volume:23 |g year:2023 |g number:1 |g pages:1-16 |g extent:16 |a Hepatic passaging of NRAS-mutant melanoma influences adhesive properties and metastatic pattern |
| 856 | 4 | 0 | |u https://doi.org/10.1186/s12885-023-10912-4 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://bmccancer.biomedcentral.com/articles/10.1186/s12885-023-10912-4 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20240807 | ||
| 993 | |a Article | ||
| 994 | |a 2023 | ||
| 998 | |g 1109821379 |a Wohlfeil, Sebastian A. |m 1109821379:Wohlfeil, Sebastian A. |d 60000 |d 61900 |e 60000PW1109821379 |e 61900PW1109821379 |k 0/60000/ |k 1/60000/61900/ |p 8 |y j | ||
| 998 | |g 137867808 |a Géraud, Cyrill |m 137867808:Géraud, Cyrill |d 60000 |d 61900 |e 60000PG137867808 |e 61900PG137867808 |k 0/60000/ |k 1/60000/61900/ |p 7 | ||
| 998 | |g 1027602177 |a Goerdt, Sergij |m 1027602177:Goerdt, Sergij |d 60000 |d 61900 |e 60000PG1027602177 |e 61900PG1027602177 |k 0/60000/ |k 1/60000/61900/ |p 6 | ||
| 998 | |g 1122355831 |a Torre, Carolina de la |m 1122355831:Torre, Carolina de la |d 60000 |d 60200 |d 60210 |e 60000PT1122355831 |e 60200PT1122355831 |e 60210PT1122355831 |k 0/60000/ |k 1/60000/60200/ |k 2/60000/60200/60210/ |p 4 | ||
| 998 | |g 1020115718 |a Sticht, Carsten |m 1020115718:Sticht, Carsten |d 60000 |d 60200 |d 60210 |e 60000PS1020115718 |e 60200PS1020115718 |e 60210PS1020115718 |k 0/60000/ |k 1/60000/60200/ |k 2/60000/60200/60210/ |p 3 | ||
| 998 | |g 1251329381 |a Weller, Céline |m 1251329381:Weller, Céline |d 60000 |d 61900 |e 60000PW1251329381 |e 61900PW1251329381 |k 0/60000/ |k 1/60000/61900/ |p 2 | ||
| 998 | |g 1251327389 |a Dietsch, Bianca |m 1251327389:Dietsch, Bianca |d 60000 |d 61900 |e 60000PD1251327389 |e 61900PD1251327389 |k 0/60000/ |k 1/60000/61900/ |p 1 |x j | ||
| 999 | |a KXP-PPN1898058423 |e 4563536954 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"name":{"displayForm":["Bianca Dietsch, Céline Weller, Carsten Sticht, Carolina de la Torre, Martin Kramer, Sergij Goerdt, Cyrill Géraud and Sebastian A. Wohlfeil"]},"note":["Gesehen am 07.08.2024"],"language":["eng"],"physDesc":[{"noteIll":"Illustrationen","extent":"16 S."}],"title":[{"title_sort":"Hepatic passaging of NRAS-mutant melanoma influences adhesive properties and metastatic pattern","title":"Hepatic passaging of NRAS-mutant melanoma influences adhesive properties and metastatic pattern"}],"recId":"1898058423","id":{"eki":["1898058423"],"doi":["10.1186/s12885-023-10912-4"]},"origin":[{"dateIssuedKey":"2023","dateIssuedDisp":"13 May 2023"}],"relHost":[{"origin":[{"publisher":"BioMed Central ; Springer","dateIssuedKey":"2001","dateIssuedDisp":"2001-","publisherPlace":"London ; Berlin ; Heidelberg"}],"id":{"issn":["1471-2407"],"zdb":["2041352-X"],"eki":["326643710"]},"title":[{"title_sort":"BMC cancer","title":"BMC cancer"}],"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"note":["Gesehen am 22.05.20"],"recId":"326643710","type":{"media":"Online-Ressource","bibl":"periodical"},"pubHistory":["1.2001 -"],"part":{"text":"23(2023), 1, Seite 1-16","extent":"16","year":"2023","issue":"1","pages":"1-16","volume":"23"},"disp":"Hepatic passaging of NRAS-mutant melanoma influences adhesive properties and metastatic patternBMC cancer"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"person":[{"role":"aut","display":"Dietsch, Bianca","family":"Dietsch","given":"Bianca"},{"given":"Céline","display":"Weller, Céline","family":"Weller","role":"aut"},{"given":"Carsten","display":"Sticht, Carsten","family":"Sticht","role":"aut"},{"role":"aut","family":"Torre","display":"Torre, Carolina de la","given":"Carolina de la"},{"given":"Martin","role":"aut","family":"Kramer","display":"Kramer, Martin"},{"given":"Sergij","role":"aut","display":"Goerdt, Sergij","family":"Goerdt"},{"given":"Cyrill","role":"aut","family":"Géraud","display":"Géraud, Cyrill"},{"role":"aut","display":"Wohlfeil, Sebastian A.","family":"Wohlfeil","given":"Sebastian A."}]} | ||
| SRT | |a DIETSCHBIAHEPATICPAS1320 | ||