Malaria parasite centrins can assemble by Ca2+-inducible condensation
Centrins are small calcium-binding proteins that have a variety of roles and are universally associated with eukaryotic centrosomes. Rapid proliferation of the malaria-causing parasite Plasmodium falciparum in the human blood depends on a particularly divergent and acentriolar centrosome, which inco...
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| Hauptverfasser: | , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
Dezember 2023
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| In: |
PLoS pathogens
Year: 2023, Jahrgang: 19, Heft: 12, Pages: 1-30 |
| ISSN: | 1553-7374 |
| DOI: | 10.1371/journal.ppat.1011899 |
| Online-Zugang: | Resolving-System, kostenfrei, Volltext: https://doi.org/10.1371/journal.ppat.1011899 Verlag, kostenfrei, Volltext: https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1011899 
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| Verfasserangaben: | Yannik Voß, Severina Klaus, Nicolas P. Lichti, Markus Ganter, Julien Guizetti |
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| 520 | |a Centrins are small calcium-binding proteins that have a variety of roles and are universally associated with eukaryotic centrosomes. Rapid proliferation of the malaria-causing parasite Plasmodium falciparum in the human blood depends on a particularly divergent and acentriolar centrosome, which incorporates several essential centrins. Their precise mode of action, however, remains unclear. In this study calcium-inducible liquid-liquid phase separation is revealed as an evolutionarily conserved principle of assembly for multiple centrins from P. falciparum and other species. Furthermore, the disordered N-terminus and calcium-binding motifs are defined as essential features for reversible biomolecular condensation, and we demonstrate that certain centrins can form co-condensates. In vivo analysis using live cell STED microscopy shows liquid-like dynamics of centrosomal centrin. Additionally, implementation of an inducible protein overexpression system reveals concentration-dependent formation of extra-centrosomal centrin assemblies with condensate-like properties. The timing of foci formation and dissolution suggests that centrin assembly is regulated. This study thereby provides a new model for centrin accumulation at eukaryotic centrosomes. | ||
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| 650 | 4 | |a CENTROSOME | |
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| 650 | 4 | |a PHOSPHORYLATION | |
| 650 | 4 | |a PLASMODIUM-FALCIPARUM | |
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