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ReLo is a simple and rapid colocalization assay to identify and characterize direct protein-protein interactions

The characterization of protein-protein interactions (PPIs) is fundamental to the understanding of biochemical processes. Many methods have been established to identify and study direct PPIs; however, screening and investigating PPIs involving large or poorly soluble proteins remains challenging. He...

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Hauptverfasser: Salgania, Harpreet Kaur (VerfasserIn) , Metz, Jutta (VerfasserIn) , Jeske, Mandy (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2024
In: Nature Communications
Year: 2024, Jahrgang: 15, Pages: 1-12
ISSN:2041-1723
DOI:10.1038/s41467-024-47233-4
Online-Zugang:Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41467-024-47233-4
Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41467-024-47233-4
Volltext
Verfasserangaben:Harpreet Kaur Salgania, Jutta Metz & Mandy Jeske
Beschreibung
Zusammenfassung:The characterization of protein-protein interactions (PPIs) is fundamental to the understanding of biochemical processes. Many methods have been established to identify and study direct PPIs; however, screening and investigating PPIs involving large or poorly soluble proteins remains challenging. Here, we introduce ReLo, a simple, rapid, and versatile cell culture-based method for detecting and investigating interactions in a cellular context. Our experiments demonstrate that ReLo specifically detects direct binary PPIs. Furthermore, we show that ReLo bridging experiments can also be used to determine the binding topology of subunits within multiprotein complexes. In addition, ReLo facilitates the identification of protein domains that mediate complex formation, allows screening for interfering point mutations, and it is sensitive to drugs that mediate or disrupt an interaction. In summary, ReLo is a simple and rapid alternative for the study of PPIs, especially when studying structurally complex proteins or when established methods fail.
Beschreibung:Online veröffentlicht: 03 April 2024
Gesehen am 21.08.2024
Beschreibung:Online Resource
ISSN:2041-1723
DOI:10.1038/s41467-024-47233-4

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