Combination of µCT and light microscopy for generation-specific stereological analysis of pulmonary arterial branches: a proof-of-concept study
Various lung diseases, including pulmonary hypertension, chronic obstructive pulmonary disease or bronchopulmonary dysplasia, are associated with structural and architectural alterations of the pulmonary vasculature. The light microscopic (LM) analysis of the blood vessels is limited by the fact tha...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2021
|
| In: |
Histochemistry and cell biology
Year: 2021, Jahrgang: 155, Heft: 2, Pages: 227-239 |
| ISSN: | 1432-119X |
| DOI: | 10.1007/s00418-020-01946-x |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1007/s00418-020-01946-x Verlag, kostenfrei, Volltext: https://link.springer.com/article/10.1007/s00418-020-01946-x 
|
| Verfasserangaben: | Roman Grothausmann, Jonas Labode, Pablo Hernandez-Cerdan, David Haberthür, Ruslan Hlushchuk, Oleg Lobachev, Christina Brandenberger, Andre George Gie, Thomas Salaets, Jaan Toelen, Willi L. Wagner, Christian Mühlfeld |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1903532914 | ||
| 003 | DE-627 | ||
| 005 | 20250325052811.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240926s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00418-020-01946-x |2 doi | |
| 035 | |a (DE-627)1903532914 | ||
| 035 | |a (DE-599)KXP1903532914 | ||
| 035 | |a (OCoLC)1475313370 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Grothausmann, Roman |e VerfasserIn |0 (DE-588)1020208015 |0 (DE-627)687770718 |0 (DE-576)359851681 |4 aut | |
| 245 | 1 | 0 | |a Combination of µCT and light microscopy for generation-specific stereological analysis of pulmonary arterial branches |b a proof-of-concept study |c Roman Grothausmann, Jonas Labode, Pablo Hernandez-Cerdan, David Haberthür, Ruslan Hlushchuk, Oleg Lobachev, Christina Brandenberger, Andre George Gie, Thomas Salaets, Jaan Toelen, Willi L. Wagner, Christian Mühlfeld |
| 246 | 1 | |i Titel des Zeitschriftenheftes |a In focus: 3D imaging in lung biology | |
| 246 | 3 | 0 | |a mu-CT |
| 246 | 3 | 3 | |a Combination of micro-CT and light microscopy for generation-specific stereological analysis of pulmonary arterial branches |
| 264 | 1 | |c 2021 | |
| 300 | |b Illustrationen | ||
| 300 | |a 13 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Online veröffentlicht: 2. Dezember 2020 | ||
| 500 | |a Gesehen am 26.09.2024 | ||
| 520 | |a Various lung diseases, including pulmonary hypertension, chronic obstructive pulmonary disease or bronchopulmonary dysplasia, are associated with structural and architectural alterations of the pulmonary vasculature. The light microscopic (LM) analysis of the blood vessels is limited by the fact that it is impossible to identify which generation of the arterial tree an arterial profile within a LM microscopic section belongs to. Therefore, we established a workflow that allows for the generation-specific quantitative (stereological) analysis of pulmonary blood vessels. A whole left rabbit lung was fixed by vascular perfusion, embedded in glycol methacrylate and imaged by micro-computed tomography (µCT). The lung was then exhaustively sectioned and 20 consecutive sections were collected every 100 µm to obtain a systematic uniform random sample of the whole lung. The digital processing involved segmentation of the arterial tree, generation analysis, registration of LM sections with the µCT data as well as registration of the segmentation and the LM images. The present study demonstrates that it is feasible to identify arterial profiles according to their generation based on a generation-specific color code. Stereological analysis for the first three arterial generations of the monopodial branching of the vasculature included volume fraction, total volume, lumen-to-wall ratio and wall thickness for each arterial generation. In conclusion, the correlative image analysis of µCT and LM-based datasets is an innovative method to assess the pulmonary vasculature quantitatively. | ||
| 650 | 4 | |a 3D reconstruction | |
| 650 | 4 | |a Branching generation analysis | |
| 650 | 4 | |a Light microscopy | |
| 650 | 4 | |a Medical Imaging | |
| 650 | 4 | |a Micro computed tomography | |
| 650 | 4 | |a Pulmonary vasculature | |
| 650 | 4 | |a Stereology | |
| 700 | 1 | |a Labode, Jonas |e VerfasserIn |4 aut | |
| 700 | 1 | |a Hernandez-Cerdan, Pablo |e VerfasserIn |4 aut | |
| 700 | 1 | |a Haberthür, David |e VerfasserIn |4 aut | |
| 700 | 1 | |a Hlushchuk, Ruslan |e VerfasserIn |4 aut | |
| 700 | 1 | |a Lobachev, Oleg |e VerfasserIn |4 aut | |
| 700 | 1 | |a Brandenberger, Christina |e VerfasserIn |4 aut | |
| 700 | 1 | |a Gie, Andre George |e VerfasserIn |4 aut | |
| 700 | 1 | |a Salaets, Thomas |e VerfasserIn |4 aut | |
| 700 | 1 | |a Toelen, Jaan |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wagner, Willi Linus |e VerfasserIn |0 (DE-588)1160332487 |0 (DE-627)1023485877 |0 (DE-576)505858754 |4 aut | |
| 700 | 1 | |8 1\p |a Mühlfeld, Christian Axel |d 1976- |e VerfasserIn |0 (DE-588)129323667 |0 (DE-627)393096203 |0 (DE-576)297598589 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Histochemistry and cell biology |d Berlin : Springer, 1995 |g 155(2021), 2 vom: Feb., Seite 227-239 |h Online-Ressource |w (DE-627)235503568 |w (DE-600)1398345-3 |w (DE-576)061936065 |x 1432-119X |7 nnas |a Combination of µCT and light microscopy for generation-specific stereological analysis of pulmonary arterial branches a proof-of-concept study |
| 773 | 1 | 8 | |g volume:155 |g year:2021 |g number:2 |g month:02 |g pages:227-239 |g extent:13 |a Combination of µCT and light microscopy for generation-specific stereological analysis of pulmonary arterial branches a proof-of-concept study |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00418-020-01946-x |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://link.springer.com/article/10.1007/s00418-020-01946-x |x Verlag |z kostenfrei |3 Volltext |
| 883 | |8 1\p |a cgwrk |d 20250301 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 951 | |a AR | ||
| 992 | |a 20240926 | ||
| 993 | |a Article | ||
| 994 | |a 2021 | ||
| 998 | |g 1160332487 |a Wagner, Willi Linus |m 1160332487:Wagner, Willi Linus |d 910000 |d 911400 |e 910000PW1160332487 |e 911400PW1160332487 |k 0/910000/ |k 1/910000/911400/ |p 11 | ||
| 999 | |a KXP-PPN1903532914 |e 4583602472 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"title":[{"subtitle":"official journal of the Society for Histochemistry","title":"Histochemistry and cell biology","title_sort":"Histochemistry and cell biology"}],"part":{"pages":"227-239","issue":"2","year":"2021","extent":"13","text":"155(2021), 2 vom: Feb., Seite 227-239","volume":"155"},"pubHistory":["Volume 103, issue 1 (January 1995)-"],"language":["eng"],"recId":"235503568","disp":"Combination of µCT and light microscopy for generation-specific stereological analysis of pulmonary arterial branches a proof-of-concept studyHistochemistry and cell biology","type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 06.02.25","Fortsetzung der Druck-Ausgabe"],"id":{"issn":["1432-119X"],"zdb":["1398345-3"],"eki":["235503568"]},"origin":[{"publisherPlace":"Berlin ; Heidelberg","dateIssuedDisp":"1995-","dateIssuedKey":"1995","publisher":"Springer"}],"physDesc":[{"extent":"Online-Ressource"}]}],"physDesc":[{"noteIll":"Illustrationen","extent":"13 S."}],"name":{"displayForm":["Roman Grothausmann, Jonas Labode, Pablo Hernandez-Cerdan, David Haberthür, Ruslan Hlushchuk, Oleg Lobachev, Christina Brandenberger, Andre George Gie, Thomas Salaets, Jaan Toelen, Willi L. Wagner, Christian Mühlfeld"]},"id":{"eki":["1903532914"],"doi":["10.1007/s00418-020-01946-x"]},"origin":[{"dateIssuedKey":"2021","dateIssuedDisp":"2021"}],"language":["eng"],"recId":"1903532914","note":["Online veröffentlicht: 2. Dezember 2020","Gesehen am 26.09.2024"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"titleAlt":[{"title":"In focus: 3D imaging in lung biology"},{"title":"Combination of micro-CT and light microscopy for generation-specific stereological analysis of pulmonary arterial branches"}],"person":[{"family":"Grothausmann","given":"Roman","display":"Grothausmann, Roman","roleDisplay":"VerfasserIn","role":"aut"},{"given":"Jonas","family":"Labode","role":"aut","display":"Labode, Jonas","roleDisplay":"VerfasserIn"},{"family":"Hernandez-Cerdan","given":"Pablo","roleDisplay":"VerfasserIn","display":"Hernandez-Cerdan, Pablo","role":"aut"},{"family":"Haberthür","given":"David","roleDisplay":"VerfasserIn","display":"Haberthür, David","role":"aut"},{"roleDisplay":"VerfasserIn","display":"Hlushchuk, Ruslan","role":"aut","family":"Hlushchuk","given":"Ruslan"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Lobachev, Oleg","given":"Oleg","family":"Lobachev"},{"display":"Brandenberger, Christina","roleDisplay":"VerfasserIn","role":"aut","family":"Brandenberger","given":"Christina"},{"role":"aut","display":"Gie, Andre George","roleDisplay":"VerfasserIn","given":"Andre George","family":"Gie"},{"given":"Thomas","family":"Salaets","role":"aut","roleDisplay":"VerfasserIn","display":"Salaets, Thomas"},{"given":"Jaan","family":"Toelen","role":"aut","display":"Toelen, Jaan","roleDisplay":"VerfasserIn"},{"family":"Wagner","given":"Willi Linus","roleDisplay":"VerfasserIn","display":"Wagner, Willi Linus","role":"aut"},{"given":"Christian Axel","family":"Mühlfeld","role":"aut","roleDisplay":"VerfasserIn","display":"Mühlfeld, Christian Axel"}],"title":[{"title_sort":"Combination of µCT and light microscopy for generation-specific stereological analysis of pulmonary arterial branches","subtitle":"a proof-of-concept study","title":"Combination of µCT and light microscopy for generation-specific stereological analysis of pulmonary arterial branches"}]} | ||
| SRT | |a GROTHAUSMACOMBINATIO2021 | ||