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Heterogeneity of deletions involving RB-1 and the D13S25 locus in B-cell chronic lymphocytic leukemia revealed by fluorescence in situ hybridization1

Recently, the D13S25 locus, which is in close proximity to the retinoblastoma gene (RB-1) on chromosome band 13q14, was discussed to play a role in the pathogenesis of B-CLL. In the present study, we isolated two overlapping genomic DNA clones (termed c13S25) containing the D13S25 DNA segment and us...

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Bibliographische Detailangaben
Hauptverfasser: Stilgenbauer, Stephan (VerfasserIn) , Leupolt, Elke (VerfasserIn) , Ohl, Sybille (VerfasserIn) , Weiß, Gunther (VerfasserIn) , Schröder, Martin (VerfasserIn) , Döhner, Konstanze (VerfasserIn) , Bentz, Martin (VerfasserIn) , Lichter, Peter (VerfasserIn) , Döhner, Hartmut (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: August 01 1995
In: Cancer research
Year: 1995, Jahrgang: 55, Heft: 16, Pages: 3475-3477
ISSN:1538-7445
Online-Zugang: Volltext
Verfasserangaben:Stephan Stilgenbauer, Elke Leupolt, Sybille Ohl, Gunther Weiß, Martin Schröder, Konstanze Fischer, Martin Bentz, Peter Lichter, Hartmut Döhner
Beschreibung
Zusammenfassung:Recently, the D13S25 locus, which is in close proximity to the retinoblastoma gene (RB-1) on chromosome band 13q14, was discussed to play a role in the pathogenesis of B-CLL. In the present study, we isolated two overlapping genomic DNA clones (termed c13S25) containing the D13S25 DNA segment and used them as probes to analyze 85 B-CLL cases by fluorescence in situ hybridization; of the 55 cases with two RB-1 copies, 13 exhibited hemizygous (n = 7) or homozygous (n = 6) deletion of D13S25. Of 29 cases with hemizygous deletion of RB-1, all but two also showed loss of D13S25 (hemizygous, n = 25; homozygous, n = 2). One case had a homozygous deletion of both loci. We conclude that deletion of D13S25 occurs in a substantial number of B-CLL without deletion of RB-1. However, in some cases there is deletion of RB-1 without loss of D13S25, suggesting that D13S25 is not the locus of the putative tumor suppressor gene. According to our data, such a gene is most likely located within the genomic region between D13S25 and RB-1.
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Beschreibung:Online Resource
ISSN:1538-7445

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