The structure of the γ-TuRC at the microtubule minus end: not just one solution
In cells, microtubules (MTs) assemble from α/β-tubulin subunits at nucleation sites containing the γ-tubulin ring complex (γ-TuRC). Within the γ-TuRC, exposed γ-tubulin molecules act as templates for MT assembly by interacting with α/β-tubulin. The vertebrate γ-TuRC is scaffolded by γ-tubulin-intera...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
23 July 2024
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| In: |
Bioessays
Year: 2024, Volume: 46, Issue: 9, Pages: 1-11 |
| ISSN: | 1521-1878 |
| DOI: | 10.1002/bies.202400117 |
| Online Access: | Verlag, kostenfrei, Volltext: https://doi.org/10.1002/bies.202400117 Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/bies.202400117 
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| Author Notes: | Qi Gao, Bram J. A. Vermeulen, Martin Würtz, Hyesu Shin, Dilara Erdogdu, Anjun Zheng, Florian W. Hofer, Annett Neuner, Stefan Pfeffer, Elmar Schiebel |
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| 520 | |a In cells, microtubules (MTs) assemble from α/β-tubulin subunits at nucleation sites containing the γ-tubulin ring complex (γ-TuRC). Within the γ-TuRC, exposed γ-tubulin molecules act as templates for MT assembly by interacting with α/β-tubulin. The vertebrate γ-TuRC is scaffolded by γ-tubulin-interacting proteins GCP2-6 arranged in a specific order. Interestingly, the γ-tubulin molecules in the γ-TuRC deviate from the cylindrical geometry of MTs, raising the question of how the γ-TuRC structure changes during MT nucleation. Recent studies on the structure of the vertebrate γ-TuRC attached to the end of MTs came to varying conclusions. In vitro assembly of MTs, facilitated by an α-tubulin mutant, resulted in a closed, cylindrical γ-TuRC showing canonical interactions between all γ-tubulin molecules and α/β-tubulin subunits. Conversely, native MTs formed in a frog extract were capped by a partially closed γ-TuRC, with some γ-tubulin molecules failing to align with α/β-tubulin. This review discusses these outcomes, along with the broader implications. | ||
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