Subunit-specific inhibition of inward-rectifier K+ channels by quinidine
Distinct inward-rectifier K+ channel subunits were expressed in Xenopus oocytes and tested for their sensitivity to the channel blocker quinidine. The ‘strong’ inward-rectifier K+ channel IRK1 was inhibited by quinidine with an EC50 of 0.7 mM, while the ‘weak’ reactifier channel ROMK1 was only moder...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
November 20, 1995
|
| In: |
FEBS letters
Year: 1995, Jahrgang: 375, Heft: 3, Pages: 193-196 |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/0014-5793(95)01182-E |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/0014-5793(95)01182-E Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1016/0014-5793%2895%2901182-E 
|
| Verfasserangaben: | T. Doi, B. Fakler, J.-H. Schultz, H. Ehmke, U. Brändle, H.-P. Zenner, H. Süßbrich, F. Lang, J.P. Ruppersberg, A.E. Busch |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1909455512 | ||
| 003 | DE-627 | ||
| 005 | 20241205222621.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 241125s1995 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/0014-5793(95)01182-E |2 doi | |
| 035 | |a (DE-627)1909455512 | ||
| 035 | |a (DE-599)KXP1909455512 | ||
| 035 | |a (OCoLC)1475647962 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Doi, Tadashi |e VerfasserIn |0 (DE-588)1349275174 |0 (DE-627)1909455210 |4 aut | |
| 245 | 1 | 0 | |a Subunit-specific inhibition of inward-rectifier K+ channels by quinidine |c T. Doi, B. Fakler, J.-H. Schultz, H. Ehmke, U. Brändle, H.-P. Zenner, H. Süßbrich, F. Lang, J.P. Ruppersberg, A.E. Busch |
| 264 | 1 | |c November 20, 1995 | |
| 300 | |a 4 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Elektronische Reproduktion der Druck-Ausgabe 9. März 2000 | ||
| 500 | |a Gesehen am 25.11.2024 | ||
| 520 | |a Distinct inward-rectifier K+ channel subunits were expressed in Xenopus oocytes and tested for their sensitivity to the channel blocker quinidine. The ‘strong’ inward-rectifier K+ channel IRK1 was inhibited by quinidine with an EC50 of 0.7 mM, while the ‘weak’ reactifier channel ROMK1 was only moderately inhibited. ROMK1(N171D)-IRKIC-term chimeric channels, which carry both sites for strong rectification of IRK1 channels (the negatively charged D171 in the second transmembrane domain and the IRK1-C-terminus including E224), displayed strong rectification like IRK1, but showed weak sensitivity to quinidine-like ROMK1, suggesting independence of quinidine binding and rectification mechanisms. Moreover, BIR10 and BIR11, two strong rectifier subunits originally cloned from rat brain, exerted subunit-specific sensitivity to quinidine, being much higher for BIR11. Quinidine blockade of IRK1 was not voltage-dependent, but strongly dependent on the pH in the superfusate. These results strongly suggest a subunit-specific interaction of inward-rectifier K+ channels with neutral quinidine within membrane lipid bilayers. | ||
| 650 | 4 | |a Inward rectifier K+ channel | |
| 650 | 4 | |a pH | |
| 650 | 4 | |a Quinidine | |
| 700 | 1 | |a Fakler, B. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schultz, Jobst-Hendrik |e VerfasserIn |0 (DE-588)1033481696 |0 (DE-627)741303361 |0 (DE-576)170664899 |4 aut | |
| 700 | 1 | |a Ehmke, Heimo |e VerfasserIn |0 (DE-588)1027511597 |0 (DE-627)729273695 |0 (DE-576)373290314 |4 aut | |
| 700 | 1 | |a Brändle, U. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Zenner, H.-P. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Süßbrich, H. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Lang, F. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Ruppersberg, J.P. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Busch, A.E. |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |a Vereinigung der Europäischen Biochemischen Gesellschaften |t FEBS letters |d Chichester : Wiley, 1968 |g 375(1995), 3, Seite 193-196 |h Online-Ressource |w (DE-627)253779669 |w (DE-600)1460391-3 |w (DE-576)07279383X |x 1873-3468 |7 nnas |
| 773 | 1 | 8 | |g volume:375 |g year:1995 |g number:3 |g pages:193-196 |g extent:4 |a Subunit-specific inhibition of inward-rectifier K+ channels by quinidine |
| 856 | 4 | 0 | |u https://doi.org/10.1016/0014-5793(95)01182-E |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://onlinelibrary.wiley.com/doi/abs/10.1016/0014-5793%2895%2901182-E |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20241125 | ||
| 993 | |a Article | ||
| 994 | |a 1995 | ||
| 998 | |g 1027511597 |a Ehmke, Heimo |m 1027511597:Ehmke, Heimo |d 50000 |d 53200 |e 50000PE1027511597 |e 53200PE1027511597 |k 0/50000/ |k 1/50000/53200/ |p 4 | ||
| 998 | |g 1033481696 |a Schultz, Jobst-Hendrik |m 1033481696:Schultz, Jobst-Hendrik |d 50000 |e 50000PS1033481696 |k 0/50000/ |p 3 | ||
| 999 | |a KXP-PPN1909455512 |e 4622130351 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"role":"aut","family":"Doi","display":"Doi, Tadashi","given":"Tadashi"},{"given":"B.","family":"Fakler","role":"aut","display":"Fakler, B."},{"family":"Schultz","role":"aut","display":"Schultz, Jobst-Hendrik","given":"Jobst-Hendrik"},{"given":"Heimo","family":"Ehmke","role":"aut","display":"Ehmke, Heimo"},{"given":"U.","display":"Brändle, U.","role":"aut","family":"Brändle"},{"role":"aut","family":"Zenner","display":"Zenner, H.-P.","given":"H.-P."},{"given":"H.","role":"aut","family":"Süßbrich","display":"Süßbrich, H."},{"given":"F.","role":"aut","family":"Lang","display":"Lang, F."},{"given":"J.P.","role":"aut","family":"Ruppersberg","display":"Ruppersberg, J.P."},{"given":"A.E.","display":"Busch, A.E.","role":"aut","family":"Busch"}],"language":["eng"],"note":["Elektronische Reproduktion der Druck-Ausgabe 9. März 2000","Gesehen am 25.11.2024"],"name":{"displayForm":["T. Doi, B. Fakler, J.-H. Schultz, H. Ehmke, U. Brändle, H.-P. Zenner, H. Süßbrich, F. Lang, J.P. Ruppersberg, A.E. Busch"]},"origin":[{"dateIssuedKey":"1995","dateIssuedDisp":"November 20, 1995"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"id":{"doi":["10.1016/0014-5793(95)01182-E"],"eki":["1909455512"]},"recId":"1909455512","title":[{"title":"Subunit-specific inhibition of inward-rectifier K+ channels by quinidine","title_sort":"Subunit-specific inhibition of inward-rectifier K+ channels by quinidine"}],"physDesc":[{"extent":"4 S."}],"relHost":[{"id":{"issn":["1873-3468"],"eki":["253779669"],"zdb":["1460391-3"],"doi":["10.1002/(ISSN)1873-3468"]},"disp":"Vereinigung der Europäischen Biochemischen GesellschaftenFEBS letters","origin":[{"dateIssuedKey":"1968","publisherPlace":"Chichester ; Amsterdam ; Amsterdam [u.a.]","dateIssuedDisp":"1968-","publisher":"Wiley ; North-Holland Publ. Co. ; Elsevier"}],"corporate":[{"display":"Vereinigung der Europäischen Biochemischen Gesellschaften","role":"aut"}],"note":["Gesehen am 18.12.2019","Fortsetzung der Druck-Ausgabe"],"pubHistory":["1.1968 -"],"title":[{"title_sort":"FEBS letters","subtitle":"for the rapid publication of short reports in biochemistry, biophysics, and molecular biology","title":"FEBS letters"}],"recId":"253779669","type":{"bibl":"periodical","media":"Online-Ressource"},"name":{"displayForm":["Federation of European Biochemical Societies. Ed. M. Avron [u.a.]"]},"language":["eng"],"part":{"pages":"193-196","year":"1995","issue":"3","extent":"4","text":"375(1995), 3, Seite 193-196","volume":"375"},"physDesc":[{"extent":"Online-Ressource"}]}]} | ||
| SRT | |a DOITADASHISUBUNITSPE2019 | ||