Differentiation and characterization of osteoclasts from human induced pluripotent stem cells

This protocol details the propagation and passaging of human iPSCs and their differentiation into osteoclasts. First, iPSCs are dissociated into a single-cell suspension for further use in embryoid body induction. Following mesodermal induction, embryoid bodies undergo hematopoietic differentiation,...

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Bibliographic Details
Main Authors: Blümke, Alexander Felix (Author) , Simon, Jessica (Author) , Leber, Elizabeth (Author) , Scatena, Marta (Author) , Giachelli, Cecilia M. (Author)
Format: Article (Journal) Video
Language:English
Published: March 22nd, 2024
In: JoVE. Video journal
Year: 2024, Issue: 205
ISSN:1940-087X
DOI:10.3791/66527
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Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3791/66527
Verlag, lizenzpflichtig, Volltext: https://app.jove.com/t/66527/differentiation-characterization-osteoclasts-from-human-induced
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Author Notes:Alexander Blümke, Jessica Simon, Elizabeth Leber, Marta Scatena, Cecilia M. Giachelli
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Differentiation and characterization of osteoclasts from human induced pluripotent stem cells by Blümke, Alexander Felix (Author) , Simon, Jessica (Author) , Leber, Elizabeth (Author) , Scatena, Marta (Author) , Giachelli, Cecilia M. (Author) ,


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Article (Journal) Video Online Resource