Differentiation and characterization of osteoclasts from human induced pluripotent stem cells
This protocol details the propagation and passaging of human iPSCs and their differentiation into osteoclasts. First, iPSCs are dissociated into a single-cell suspension for further use in embryoid body induction. Following mesodermal induction, embryoid bodies undergo hematopoietic differentiation,...
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| Main Authors: | , , , , |
|---|---|
| Format: | Article (Journal) Video |
| Language: | English |
| Published: |
March 22nd, 2024
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| In: |
JoVE. Video journal
Year: 2024, Issue: 205 |
| ISSN: | 1940-087X |
| DOI: | 10.3791/66527 |
| Subjects: | |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3791/66527 Verlag, lizenzpflichtig, Volltext: https://app.jove.com/t/66527/differentiation-characterization-osteoclasts-from-human-induced |
| Author Notes: | Alexander Blümke, Jessica Simon, Elizabeth Leber, Marta Scatena, Cecilia M. Giachelli |
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