Buchumschlag

Isolation of peroxisome subpopulations from rat liver by means of immune free-flow electrophoresis

Immune free-flow electrophoresis (IFFE) has been applied to the separation of peroxisomes (PO). IFFE is a modification of antigen-specific electrophoretic cell separation (ASECS), and combines the advantages of electrophoretic separation with the high selectivity of an immune reaction. It differs fr...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Völkl, Alfred (VerfasserIn) , Mohr, Heribert (VerfasserIn) , Weber, Gerhard (VerfasserIn) , Fahimi, H. Dariush (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: June 1998
In: Electrophoresis
Year: 1998, Jahrgang: 19, Heft: 7, Pages: 1140-1144
ISSN:1522-2683
DOI:10.1002/elps.1150190714
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/elps.1150190714
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.1150190714
Volltext
Verfasserangaben:Alfred Völkl, Heribert Mohr, Gerhard Weber, H. Dariush Fahimi
Beschreibung
Zusammenfassung:Immune free-flow electrophoresis (IFFE) has been applied to the separation of peroxisomes (PO). IFFE is a modification of antigen-specific electrophoretic cell separation (ASECS), and combines the advantages of electrophoretic separation with the high selectivity of an immune reaction. It differs from the latter in the pH of the electrophoresis buffer, which was shifted from the physiological range (ASECS) to the pI of IgG molecules (pH ∼ 8.0), thus further decreasing the mobility produced by the binding of a specific antibody. This enhances the mobility differences between IgG-coupled particles and those nondecorated, with resultant improved separation. We have now succeeded in isolating different subpopulations of PO by applying IFFE to heavy, light, and post-mitochondrial fractions separated by differential centrifugation of a rat liver homogenate. The obtained PO subfractions differed in their composition of matrix and membrane proteins, as revealed by immunoblotting. This indicates that they indeed represent distinct subpopulations of rat hepatic PO.
Beschreibung:Elektronische Reproduktion der Druck-Ausgabe 14. April 2005
Gesehen am 15.01.2025
Beschreibung:Online Resource
ISSN:1522-2683
DOI:10.1002/elps.1150190714

Ähnliche Einträge