A closer look at high-energy X-ray-induced bubble formation during soft tissue imaging
Improving the scalability of tissue imaging throughput with bright, coherent X-rays requires identifying and mitigating artifacts resulting from the interactions between X-rays and matter. At synchrotron sources, long-term imaging of soft tissues in solution can result in gas bubble formation or cav...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
May 2024
|
| In: |
Journal of synchrotron radiation
Year: 2024, Jahrgang: 31, Heft: 3, Pages: 566-577 |
| ISSN: | 1600-5775 |
| DOI: | 10.1107/S160057752400290X |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1107/S160057752400290X Verlag, kostenfrei, Volltext: https://journals.iucr.org/s/issues/2024/03/00/ing5001/ 
|
| Verfasserangaben: | R. Patrick Xian, Joseph Brunet, Yuze Huang, Willi L. Wagner, Peter D. Lee, Paul Tafforeau and Claire L. Walsh |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1915383064 | ||
| 003 | DE-627 | ||
| 005 | 20250716223755.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 250122s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1107/S160057752400290X |2 doi | |
| 035 | |a (DE-627)1915383064 | ||
| 035 | |a (DE-599)KXP1915383064 | ||
| 035 | |a (OCoLC)1528017074 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Xian, Rui |e VerfasserIn |0 (DE-588)1120318920 |0 (DE-627)873286030 |0 (DE-576)480272220 |4 aut | |
| 245 | 1 | 2 | |a A closer look at high-energy X-ray-induced bubble formation during soft tissue imaging |c R. Patrick Xian, Joseph Brunet, Yuze Huang, Willi L. Wagner, Peter D. Lee, Paul Tafforeau and Claire L. Walsh |
| 264 | 1 | |c May 2024 | |
| 300 | |b Diagramme | ||
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 22.01.2025 | ||
| 520 | |a Improving the scalability of tissue imaging throughput with bright, coherent X-rays requires identifying and mitigating artifacts resulting from the interactions between X-rays and matter. At synchrotron sources, long-term imaging of soft tissues in solution can result in gas bubble formation or cavitation, which dramatically compromises image quality and integrity of the samples. By combining in-line phase-contrast imaging with gas chromatography in real time, we were able to track the onset and evolution of high-energy X-ray-induced gas bubbles in ethanol-embedded soft tissue samples for tens of minutes (two to three times the typical scan times). We demonstrate quantitatively that vacuum degassing of the sample during preparation can significantly delay bubble formation, offering up to a twofold improvement in dose tolerance, depending on the tissue type. However, once nucleated, bubble growth is faster in degassed than undegassed samples, indicating their distinct metastable states at bubble onset. Gas chromatography analysis shows increased solvent vaporization concurrent with bubble formation, yet the quantities of dissolved gasses remain unchanged. By coupling features extracted from the radiographs with computational analysis of bubble characteristics, we uncover dose-controlled kinetics and nucleation site-specific growth. These hallmark signatures provide quantitative constraints on the driving mechanisms of bubble formation and growth. Overall, the observations highlight bubble formation as a critical yet often overlooked hurdle in upscaling X-ray imaging for biological tissues and soft materials and we offer an empirical foundation for their understanding and imaging protocol optimization. More importantly, our approaches establish a top-down scheme to decipher the complex, multiscale radiation-matter interactions in these applications. | ||
| 700 | 1 | |a Brunet, Joseph |e VerfasserIn |4 aut | |
| 700 | 1 | |a Huang, Yuze |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wagner, Willi Linus |e VerfasserIn |0 (DE-588)1160332487 |0 (DE-627)1023485877 |0 (DE-576)505858754 |4 aut | |
| 700 | 1 | |a Lee, Peter D. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Tafforeau, Paul |e VerfasserIn |4 aut | |
| 700 | 1 | |a Walsh, Claire L. |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of synchrotron radiation |d Chester : IUCr, 1994 |g 31(2024), 3, Seite 566-577 |h Online-Ressource |w (DE-627)317885510 |w (DE-600)2021413-3 |w (DE-576)095660232 |x 1600-5775 |7 nnas |a A closer look at high-energy X-ray-induced bubble formation during soft tissue imaging |
| 773 | 1 | 8 | |g volume:31 |g year:2024 |g number:3 |g pages:566-577 |g extent:12 |a A closer look at high-energy X-ray-induced bubble formation during soft tissue imaging |
| 856 | 4 | 0 | |u https://doi.org/10.1107/S160057752400290X |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://journals.iucr.org/s/issues/2024/03/00/ing5001/ |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20250122 | ||
| 993 | |a Article | ||
| 994 | |a 2024 | ||
| 998 | |g 1160332487 |a Wagner, Willi Linus |m 1160332487:Wagner, Willi Linus |d 910000 |d 911400 |e 910000PW1160332487 |e 911400PW1160332487 |k 0/910000/ |k 1/910000/911400/ |p 4 | ||
| 999 | |a KXP-PPN1915383064 |e 4655056878 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"physDesc":[{"noteIll":"Diagramme","extent":"12 S."}],"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"name":{"displayForm":["International Union of Crystallography"]},"id":{"zdb":["2021413-3"],"eki":["317885510"],"doi":["10.1107/S16005775"],"issn":["1600-5775"]},"origin":[{"dateIssuedDisp":"1994-","publisher":"IUCr ; Wiley-Blackwell","dateIssuedKey":"1994","publisherPlace":"Chester ; [Erscheinungsort nicht ermittelbar]"}],"recId":"317885510","corporate":[{"role":"isb","display":"Internationale Union für Kristallographie","roleDisplay":"Herausgebendes Organ"}],"language":["eng"],"disp":"A closer look at high-energy X-ray-induced bubble formation during soft tissue imagingJournal of synchrotron radiation","type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 24.03.2022"],"part":{"text":"31(2024), 3, Seite 566-577","volume":"31","extent":"12","year":"2024","issue":"3","pages":"566-577"},"titleAlt":[{"title":"Synchrotron radiation online"},{"title":"Synchrotron radiation"}],"pubHistory":["1.1994 -"],"title":[{"title_sort":"Journal of synchrotron radiation","title":"Journal of synchrotron radiation"}]}],"origin":[{"dateIssuedDisp":"May 2024","dateIssuedKey":"2024"}],"id":{"eki":["1915383064"],"doi":["10.1107/S160057752400290X"]},"name":{"displayForm":["R. Patrick Xian, Joseph Brunet, Yuze Huang, Willi L. Wagner, Peter D. Lee, Paul Tafforeau and Claire L. Walsh"]},"note":["Gesehen am 22.01.2025"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"language":["eng"],"recId":"1915383064","title":[{"title":"A closer look at high-energy X-ray-induced bubble formation during soft tissue imaging","title_sort":"closer look at high-energy X-ray-induced bubble formation during soft tissue imaging"}],"person":[{"display":"Xian, Rui","roleDisplay":"VerfasserIn","role":"aut","family":"Xian","given":"Rui"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Brunet, Joseph","given":"Joseph","family":"Brunet"},{"family":"Huang","given":"Yuze","display":"Huang, Yuze","roleDisplay":"VerfasserIn","role":"aut"},{"display":"Wagner, Willi Linus","roleDisplay":"VerfasserIn","role":"aut","family":"Wagner","given":"Willi Linus"},{"role":"aut","display":"Lee, Peter D.","roleDisplay":"VerfasserIn","given":"Peter D.","family":"Lee"},{"display":"Tafforeau, Paul","roleDisplay":"VerfasserIn","role":"aut","family":"Tafforeau","given":"Paul"},{"family":"Walsh","given":"Claire L.","roleDisplay":"VerfasserIn","display":"Walsh, Claire L.","role":"aut"}]} | ||
| SRT | |a XIANRUIBRUCLOSERLOOK2024 | ||