In vitro chondrogenic induction promotes the expression level of IL-10 via the TGF-β/SMAD and canonical Wnt/β-catenin signaling pathways in exosomes secreted by human adipose tissue-derived mesenchymal stem cells
Current treatment approaches cannot exactly regenerate cartilage tissue. Regarding some problems encountered with cell therapy, exosomes are advantageous because of their “cell-free” nature. This study examines the relationship between IL-10 and TGF-β and Canonical Wnt/β-catenin signal pathways in h...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
December 2024
|
| In: |
Cell biochemistry and biophysics
Year: 2024, Jahrgang: 82, Heft: 4, Pages: 3741-3750 |
| ISSN: | 1559-0283 |
| DOI: | 10.1007/s12013-024-01461-z |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s12013-024-01461-z Verlag, lizenzpflichtig, Volltext: http://link.springer.com/article/10.1007/s12013-024-01461-z 
|
| Verfasserangaben: | Tugba Semerci Sevimli, Ulukan Inan, Dilara Mantar, Kubra Guler, Zarifa Ahmadova, Kadri Gulec, Ahmet Emin Topal |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1915631068 | ||
| 003 | DE-627 | ||
| 005 | 20250716224304.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 250127s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s12013-024-01461-z |2 doi | |
| 035 | |a (DE-627)1915631068 | ||
| 035 | |a (DE-599)KXP1915631068 | ||
| 035 | |a (OCoLC)1528017245 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Semerci Sevimli, Tuğba |e VerfasserIn |0 (DE-588)1348710055 |0 (DE-627)1908936908 |4 aut | |
| 245 | 1 | 0 | |a In vitro chondrogenic induction promotes the expression level of IL-10 via the TGF-β/SMAD and canonical Wnt/β-catenin signaling pathways in exosomes secreted by human adipose tissue-derived mesenchymal stem cells |c Tugba Semerci Sevimli, Ulukan Inan, Dilara Mantar, Kubra Guler, Zarifa Ahmadova, Kadri Gulec, Ahmet Emin Topal |
| 264 | 1 | |c December 2024 | |
| 300 | |b Illustrationen | ||
| 300 | |a 10 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 27.01.2025 | ||
| 520 | |a Current treatment approaches cannot exactly regenerate cartilage tissue. Regarding some problems encountered with cell therapy, exosomes are advantageous because of their “cell-free” nature. This study examines the relationship between IL-10 and TGF-β and Canonical Wnt/β-catenin signal pathways in human adipose tissue-derived MSCs exosomes (hAT-MSCs-Exos) after in vitro chondrogenic differentiation. Human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and, as a control group, human fetal chondroblast cells (hfCCs) were differentiated chondrogenically in vitro. Exosome isolation and characterization analyses were performed. Chondrogenic differentiation was shown by Alcian Blue and Safranin O stainings. The expression levels of IL-10, TGF-β/SMAD signaling pathway genes, and Canonical Wnt/β-catenin signaling pathway genes, which play an essential role in chondrogenesis, were analyzed by RT-qPCR. Conditioned media cytokine levels were measured by using the TGF-β and IL-10 ELISA kits. IL-10 expression was upregulated in both chondrogenic differentiated hAT-MSC-Exos (dhAT-MSC-Exos) (p < 0.0001). In the TGF-β signaling pathway, TGF-β (p < 0.0001), SMAD2 (p < 0.0001), SMAD4 (p < 0.001), ACAN (p < 0.0001), SOX9 (p < 0.05) and COL1A2 (p < 0.0001) expressions were upregulated in dhAT-MSC-Exos. SMAD3 expression was upregulated in non-differentiated hAT-MSC-Exos. In the Canonical Wnt/β-catenin signaling pathway, WNT (p < 0.0001) and CTNNB1(p < 0.0001) expressions were upregulated in dhAT-MSC-Exos. AXIN (p < 0.0001) expression was upregulated in non-differentiated hAT-MSC-Exos. TGF-β and IL-10 levels were higher in dhAT-MSCs) (p < 0.0001). Related to these results, IL-10 may induce TGF-β/SMAD and Canonical Wnt/β-catenin signaling pathways in hAT-MSC exosomes obtained after chondrogenic differentiation. Therefore, using these exosomes for cartilage regeneration can lead to the development of treatment methods. | ||
| 700 | 1 | |a Inan, Ulukan |e VerfasserIn |4 aut | |
| 700 | 1 | |a Mantar, Dilara |e VerfasserIn |4 aut | |
| 700 | 1 | |a Guler, Kubra |e VerfasserIn |4 aut | |
| 700 | 1 | |a Ahmadova, Zarifa |d 1991- |e VerfasserIn |0 (DE-588)1348709200 |0 (DE-627)1908936320 |4 aut | |
| 700 | 1 | |a Gulec, Kadri |e VerfasserIn |4 aut | |
| 700 | 1 | |a Topal, Ahmet Emin |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cell biochemistry and biophysics |d New York, NY : Springer, 1995 |g 82(2024), 4 vom: Dez., Seite 3741-3750 |h Online-Ressource |w (DE-627)342893793 |w (DE-600)2072590-5 |w (DE-576)288574214 |x 1559-0283 |7 nnas |a In vitro chondrogenic induction promotes the expression level of IL-10 via the TGF-β/SMAD and canonical Wnt/β-catenin signaling pathways in exosomes secreted by human adipose tissue-derived mesenchymal stem cells |
| 773 | 1 | 8 | |g volume:82 |g year:2024 |g number:4 |g month:12 |g pages:3741-3750 |g extent:10 |a In vitro chondrogenic induction promotes the expression level of IL-10 via the TGF-β/SMAD and canonical Wnt/β-catenin signaling pathways in exosomes secreted by human adipose tissue-derived mesenchymal stem cells |
| 787 | 0 | 8 | |i Errata |a Semerci Sevimli, Tuğba |t Correction: In vitro chondrogenic induction promotes the expression level of IL-10 via the TGF-β/SMAD and canonical Wnt/β-catenin signaling pathways in exosomes secreted by human adipose tissue-derived mesenchymal stem cells |d 2024 |w (DE-627)1920336907 |
| 787 | 0 | 8 | |i Errata |a Semerci Sevimli, Tuğba |t Correction: in vitro chondrogenic induction promotes the expression level of IL-10 via the TGF-β/SMAD and canonical Wnt/β-catenin signaling pathways in exosomes secreted by human adipose tissue-derived mesenchymal stem cells |d 2025 |w (DE-627)1929331479 |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s12013-024-01461-z |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u http://link.springer.com/article/10.1007/s12013-024-01461-z |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20250127 | ||
| 993 | |a Article | ||
| 994 | |a 2024 | ||
| 998 | |g 1348709200 |a Ahmadova, Zarifa |m 1348709200:Ahmadova, Zarifa |d 60000 |d 61800 |e 60000PA1348709200 |e 61800PA1348709200 |k 0/60000/ |k 1/60000/61800/ |p 6 | ||
| 999 | |a KXP-PPN1915631068 |e 4656535176 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 27.01.2025"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"recId":"1915631068","language":["eng"],"title":[{"title_sort":"In vitro chondrogenic induction promotes the expression level of IL-10 via the TGF-β/SMAD and canonical Wnt/β-catenin signaling pathways in exosomes secreted by human adipose tissue-derived mesenchymal stem cells","title":"In vitro chondrogenic induction promotes the expression level of IL-10 via the TGF-β/SMAD and canonical Wnt/β-catenin signaling pathways in exosomes secreted by human adipose tissue-derived mesenchymal stem cells"}],"person":[{"role":"aut","display":"Semerci Sevimli, Tuğba","roleDisplay":"VerfasserIn","given":"Tuğba","family":"Semerci Sevimli"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Inan, Ulukan","given":"Ulukan","family":"Inan"},{"role":"aut","display":"Mantar, Dilara","roleDisplay":"VerfasserIn","given":"Dilara","family":"Mantar"},{"role":"aut","display":"Guler, Kubra","roleDisplay":"VerfasserIn","given":"Kubra","family":"Guler"},{"family":"Ahmadova","given":"Zarifa","roleDisplay":"VerfasserIn","display":"Ahmadova, Zarifa","role":"aut"},{"family":"Gulec","given":"Kadri","display":"Gulec, Kadri","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Topal","given":"Ahmet Emin","display":"Topal, Ahmet Emin","roleDisplay":"VerfasserIn","role":"aut"}],"physDesc":[{"extent":"10 S.","noteIll":"Illustrationen"}],"relHost":[{"title":[{"title":"Cell biochemistry and biophysics","title_sort":"Cell biochemistry and biophysics"}],"language":["eng"],"recId":"342893793","type":{"bibl":"periodical","media":"Online-Ressource"},"disp":"In vitro chondrogenic induction promotes the expression level of IL-10 via the TGF-β/SMAD and canonical Wnt/β-catenin signaling pathways in exosomes secreted by human adipose tissue-derived mesenchymal stem cellsCell biochemistry and biophysics","note":["Gesehen am 08.07.24"],"part":{"pages":"3741-3750","issue":"4","year":"2024","extent":"10","text":"82(2024), 4 vom: Dez., Seite 3741-3750","volume":"82"},"pubHistory":["Volume 27, issue 2 (1995)-"],"id":{"issn":["1559-0283"],"eki":["342893793"],"zdb":["2072590-5"]},"origin":[{"publisherPlace":"New York, NY ; Totowa, NJ","dateIssuedKey":"1995","publisher":"Springer ; Humana Press","dateIssuedDisp":"1995-"}],"physDesc":[{"extent":"Online-Ressource"}]}],"origin":[{"dateIssuedDisp":"December 2024","dateIssuedKey":"2024"}],"id":{"doi":["10.1007/s12013-024-01461-z"],"eki":["1915631068"]},"name":{"displayForm":["Tugba Semerci Sevimli, Ulukan Inan, Dilara Mantar, Kubra Guler, Zarifa Ahmadova, Kadri Gulec, Ahmet Emin Topal"]}} | ||
| SRT | |a SEMERCISEVINVITROCHO2024 | ||