Characterization of vascular niche in systemic sclerosis by spatial proteomics
BACKGROUND: - Systemic sclerosis (SSc) is a connective tissue disease that can serve as a model to study vascular changes in response to inflammation, autoimmunity, and fibrotic remodeling. Although microvascular changes are the earliest histopathologic manifestation of SSc, the vascular pathophysio...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
29 March 2024
|
| In: |
Circulation research
Year: 2024, Jahrgang: 134, Heft: 7, Pages: 875-891 |
| ISSN: | 1524-4571 |
| DOI: | 10.1161/CIRCRESAHA.123.323299 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1161/CIRCRESAHA.123.323299 Verlag, lizenzpflichtig, Volltext: https://www.ahajournals.org/doi/10.1161/CIRCRESAHA.123.323299 
|
| Verfasserangaben: | Aleix Rius Rigau, Yi-Nan Li, Alexandru-Emil Matei, Andrea-Hermina Györfi, Peter-Martin Bruch, Sarah Koziel, Veda Devakumar, Armando Gabrielli, Alexander Kreuter, Jiucun Wang, Sascha Dietrich, Georg Schett, Jörg H.W. Distler, and Minrui Liang |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1915947731 | ||
| 003 | DE-627 | ||
| 005 | 20250716225402.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 250129s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1161/CIRCRESAHA.123.323299 |2 doi | |
| 035 | |a (DE-627)1915947731 | ||
| 035 | |a (DE-599)KXP1915947731 | ||
| 035 | |a (OCoLC)1528018124 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Rigau, Aleix Rius |e VerfasserIn |0 (DE-588)1342239423 |0 (DE-627)190270360X |4 aut | |
| 245 | 1 | 0 | |a Characterization of vascular niche in systemic sclerosis by spatial proteomics |c Aleix Rius Rigau, Yi-Nan Li, Alexandru-Emil Matei, Andrea-Hermina Györfi, Peter-Martin Bruch, Sarah Koziel, Veda Devakumar, Armando Gabrielli, Alexander Kreuter, Jiucun Wang, Sascha Dietrich, Georg Schett, Jörg H.W. Distler, and Minrui Liang |
| 264 | 1 | |c 29 March 2024 | |
| 300 | |b Illustrationen, Diagramme | ||
| 300 | |a 17 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 29.01.2025 | ||
| 520 | |a BACKGROUND: - Systemic sclerosis (SSc) is a connective tissue disease that can serve as a model to study vascular changes in response to inflammation, autoimmunity, and fibrotic remodeling. Although microvascular changes are the earliest histopathologic manifestation of SSc, the vascular pathophysiology remains poorly understood. - METHODS: - We applied spatial proteomic approaches to deconvolute the heterogeneity of vascular cells at the single-cell level in situ and characterize cellular alterations of the vascular niches of patients with SSc. Skin biopsies of patients with SSc and control individuals were analyzed by imaging mass cytometry, yielding a total of 90 755 cells including 2987 endothelial cells and 4096 immune cells. - RESULTS: - We identified 7 different subpopulations of blood vascular endothelial cells (VECs), 2 subpopulations of lymphatic endothelial cells, and 3 subpopulations of pericytes. A novel population of CD34+;αSMA+ (α-smooth muscle actin);CD31+ VECs was more common in SSc, whereas endothelial precursor cells were decreased. Co-detection by indexing and tyramide signal amplification confirmed these findings. The microenvironment of CD34+;αSMA+;CD31+ VECs was enriched for immune cells and myofibroblasts, and CD34+;αSMA+;CD31+ VECs expressed markers of endothelial-to-mesenchymal transition. The density of CD34+;αSMA+;CD31+ VECs was associated with clinical progression of fibrosis in SSc. - CONCLUSIONS: - Using spatial proteomics, we unraveled the heterogeneity of vascular cells in control individuals and patients with SSc. We identified CD34+;αSMA+;CD31+ VECs as a novel endothelial cell population that is increased in patients with SSc, expresses markers for endothelial-to-mesenchymal transition, and is located in close proximity to immune cells and myofibroblasts. CD34+;αSMA+;CD31+ VEC counts were associated with clinical outcomes of progressive fibrotic remodeling, thus providing a novel cellular correlate for the crosstalk of vasculopathy and fibrosis. | ||
| 700 | 1 | |a Li, Yi-Nan |e VerfasserIn |4 aut | |
| 700 | 1 | |a Matei, Alexandru-Emil |e VerfasserIn |4 aut | |
| 700 | 1 | |a Györfi, Andrea-Hermina |e VerfasserIn |4 aut | |
| 700 | 1 | |a Bruch, Peter-Martin |d 1993- |e VerfasserIn |0 (DE-588)1203140363 |0 (DE-627)168794198X |4 aut | |
| 700 | 1 | |a Koziel, Sarah |e VerfasserIn |4 aut | |
| 700 | 1 | |a Devakumar, Veda |e VerfasserIn |4 aut | |
| 700 | 1 | |a Gabrielli, Armando |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kreuter, Alexander |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wang, Jiucun |e VerfasserIn |4 aut | |
| 700 | 1 | |a Dietrich, Sascha |d 1979- |e VerfasserIn |0 (DE-588)136406300 |0 (DE-627)694354635 |0 (DE-576)301003408 |4 aut | |
| 700 | 1 | |a Schett, Georg |e VerfasserIn |4 aut | |
| 700 | 1 | |a Distler, Jörg H.W. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Liang, Minrui |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Circulation research |d New York, NY : Assoc., 1953 |g 134(2024), 7, Seite 875-891 |h Online-Ressource |w (DE-627)266880126 |w (DE-600)1467838-X |w (DE-576)075145766 |x 1524-4571 |7 nnas |a Characterization of vascular niche in systemic sclerosis by spatial proteomics |
| 773 | 1 | 8 | |g volume:134 |g year:2024 |g number:7 |g pages:875-891 |g extent:17 |a Characterization of vascular niche in systemic sclerosis by spatial proteomics |
| 856 | 4 | 0 | |u https://doi.org/10.1161/CIRCRESAHA.123.323299 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.ahajournals.org/doi/10.1161/CIRCRESAHA.123.323299 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20250129 | ||
| 993 | |a Article | ||
| 994 | |a 2024 | ||
| 998 | |g 136406300 |a Dietrich, Sascha |m 136406300:Dietrich, Sascha |d 910000 |d 910100 |d 50000 |e 910000PD136406300 |e 910100PD136406300 |e 50000PD136406300 |k 0/910000/ |k 1/910000/910100/ |k 0/50000/ |p 11 | ||
| 998 | |g 1203140363 |a Bruch, Peter-Martin |m 1203140363:Bruch, Peter-Martin |d 50000 |e 50000PB1203140363 |k 0/50000/ |p 5 | ||
| 999 | |a KXP-PPN1915947731 |e 4658999447 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"language":["eng"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Gesehen am 29.01.2025"],"origin":[{"dateIssuedKey":"2024","dateIssuedDisp":"29 March 2024"}],"title":[{"title_sort":"Characterization of vascular niche in systemic sclerosis by spatial proteomics","title":"Characterization of vascular niche in systemic sclerosis by spatial proteomics"}],"person":[{"family":"Rigau","display":"Rigau, Aleix Rius","given":"Aleix Rius","role":"aut"},{"given":"Yi-Nan","role":"aut","family":"Li","display":"Li, Yi-Nan"},{"given":"Alexandru-Emil","role":"aut","family":"Matei","display":"Matei, Alexandru-Emil"},{"role":"aut","given":"Andrea-Hermina","display":"Györfi, Andrea-Hermina","family":"Györfi"},{"given":"Peter-Martin","role":"aut","display":"Bruch, Peter-Martin","family":"Bruch"},{"role":"aut","given":"Sarah","display":"Koziel, Sarah","family":"Koziel"},{"family":"Devakumar","display":"Devakumar, Veda","given":"Veda","role":"aut"},{"given":"Armando","role":"aut","display":"Gabrielli, Armando","family":"Gabrielli"},{"family":"Kreuter","display":"Kreuter, Alexander","role":"aut","given":"Alexander"},{"display":"Wang, Jiucun","family":"Wang","role":"aut","given":"Jiucun"},{"given":"Sascha","role":"aut","family":"Dietrich","display":"Dietrich, Sascha"},{"family":"Schett","display":"Schett, Georg","role":"aut","given":"Georg"},{"role":"aut","given":"Jörg H.W.","display":"Distler, Jörg H.W.","family":"Distler"},{"role":"aut","given":"Minrui","display":"Liang, Minrui","family":"Liang"}],"relHost":[{"note":["Gesehen am 09.08.2019"],"origin":[{"publisher":"Assoc.","dateIssuedKey":"1953","dateIssuedDisp":"1953-","publisherPlace":"New York, NY"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"title":[{"title":"Circulation research","title_sort":"Circulation research","subtitle":"an official journal of the American Heart Association"}],"part":{"issue":"7","year":"2024","pages":"875-891","text":"134(2024), 7, Seite 875-891","extent":"17","volume":"134"},"language":["eng"],"corporate":[{"display":"American Heart Association","role":"isb"}],"disp":"Characterization of vascular niche in systemic sclerosis by spatial proteomicsCirculation research","id":{"issn":["1524-4571"],"eki":["266880126"],"zdb":["1467838-X"]},"physDesc":[{"extent":"Online-Ressource"}],"pubHistory":["1.1953 -"],"recId":"266880126"}],"id":{"doi":["10.1161/CIRCRESAHA.123.323299"],"eki":["1915947731"]},"recId":"1915947731","name":{"displayForm":["Aleix Rius Rigau, Yi-Nan Li, Alexandru-Emil Matei, Andrea-Hermina Györfi, Peter-Martin Bruch, Sarah Koziel, Veda Devakumar, Armando Gabrielli, Alexander Kreuter, Jiucun Wang, Sascha Dietrich, Georg Schett, Jörg H.W. Distler, and Minrui Liang"]},"physDesc":[{"extent":"17 S.","noteIll":"Illustrationen, Diagramme"}]} | ||
| SRT | |a RIGAUALEIXCHARACTERI2920 | ||