A bistable inhibitory optoGPCR for multiplexed optogenetic control of neural circuits
Information is transmitted between brain regions through the release of neurotransmitters from long-range projecting axons. Understanding how the activity of such long-range connections contributes to behavior requires efficient methods for reversibly manipulating their function. Chemogenetic and op...
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| Hauptverfasser: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
July 2024
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| In: |
Nature methods
Year: 2024, Jahrgang: 21, Heft: 7, Pages: 1275-1287 |
| ISSN: | 1548-7105 |
| DOI: | 10.1038/s41592-024-02285-8 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41592-024-02285-8 Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41592-024-02285-8 
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| Verfasserangaben: | Jonas Wietek, Adrianna Nozownik, Mauro Pulin, Inbar Saraf-Sinik, Noa Matosevich, Raajaram Gowrishankar, Asaf Gat, Daniela Malan, Bobbie J. Brown, Julien Dine, Bibi Nusreen Imambocus, Rivka Levy, Kathrin Sauter, Anna Litvin, Noa Regev, Suraj Subramaniam, Khalid Abrera, Dustin Summarli, Eva Madeline Goren, Gili Mizrachi, Eyal Bitton, Asaf Benjamin, Bryan A. Copits, Philipp Sasse, Benjamin R. Rost, Dietmar Schmitz, Michael R. Bruchas, Peter Soba, Meital Oren-Suissa, Yuval Nir, J. Simon Wiegert & Ofer Yizhar |
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| 245 | 1 | 2 | |a A bistable inhibitory optoGPCR for multiplexed optogenetic control of neural circuits |c Jonas Wietek, Adrianna Nozownik, Mauro Pulin, Inbar Saraf-Sinik, Noa Matosevich, Raajaram Gowrishankar, Asaf Gat, Daniela Malan, Bobbie J. Brown, Julien Dine, Bibi Nusreen Imambocus, Rivka Levy, Kathrin Sauter, Anna Litvin, Noa Regev, Suraj Subramaniam, Khalid Abrera, Dustin Summarli, Eva Madeline Goren, Gili Mizrachi, Eyal Bitton, Asaf Benjamin, Bryan A. Copits, Philipp Sasse, Benjamin R. Rost, Dietmar Schmitz, Michael R. Bruchas, Peter Soba, Meital Oren-Suissa, Yuval Nir, J. Simon Wiegert & Ofer Yizhar |
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| 520 | |a Information is transmitted between brain regions through the release of neurotransmitters from long-range projecting axons. Understanding how the activity of such long-range connections contributes to behavior requires efficient methods for reversibly manipulating their function. Chemogenetic and optogenetic tools, acting through endogenous G-protein-coupled receptor pathways, can be used to modulate synaptic transmission, but existing tools are limited in sensitivity, spatiotemporal precision or spectral multiplexing capabilities. Here we systematically evaluated multiple bistable opsins for optogenetic applications and found that the Platynereis dumerilii ciliary opsin (PdCO) is an efficient, versatile, light-activated bistable G-protein-coupled receptor that can suppress synaptic transmission in mammalian neurons with high temporal precision in vivo. PdCO has useful biophysical properties that enable spectral multiplexing with other optogenetic actuators and reporters. We demonstrate that PdCO can be used to conduct reversible loss-of-function experiments in long-range projections of behaving animals, thereby enabling detailed synapse-specific functional circuit mapping. | ||
| 650 | 4 | |a Molecular neuroscience | |
| 650 | 4 | |a Neurophysiology | |
| 650 | 4 | |a Synaptic transmission | |
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