Soft X-ray tomography reveals variations in B.subtilis biofilm structure upon tasA deletion [data]
Bacterial biofilms are complex communities of cells within a self-produced extracellular matrix. They play crucial roles in healthcare, nutrition, agriculture and environmental research, yet an analysis of their elaborate 3D architecture remains challenging. In our work, we developed a “biofilm-in-c...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Database Research Data |
| Language: | English |
| Published: |
Heidelberg
Universität
2025-02-03
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| DOI: | 10.11588/DATA/KH6NDD |
| Subjects: | |
| Online Access: | Verlag, kostenfrei, Volltext: https://doi.org/10.11588/DATA/KH6NDD Verlag, kostenfrei, Volltext: https://heidata.uni-heidelberg.de/dataset.xhtml?persistentId=doi:10.11588/DATA/KH6NDD 
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| Author Notes: | Venera Weinhardt, Anthoula Chatzimpinou, Anne Diehl, A. Tobias Harhoff, Kristina Driller, Bieke Vanslembrouck, Jian-Hua Chen, Kristaps Kairiss, Valentina Loconte, Mark A. Le Gros, Carolyn Larabell, Kürşad Turgay, Hartmut Oschkinat |
MARC
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| 245 | 1 | 0 | |a Soft X-ray tomography reveals variations in B.subtilis biofilm structure upon tasA deletion [data] |c Venera Weinhardt, Anthoula Chatzimpinou, Anne Diehl, A. Tobias Harhoff, Kristina Driller, Bieke Vanslembrouck, Jian-Hua Chen, Kristaps Kairiss, Valentina Loconte, Mark A. Le Gros, Carolyn Larabell, Kürşad Turgay, Hartmut Oschkinat |
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| 520 | |a Bacterial biofilms are complex communities of cells within a self-produced extracellular matrix. They play crucial roles in healthcare, nutrition, agriculture and environmental research, yet an analysis of their elaborate 3D architecture remains challenging. In our work, we developed a “biofilm-in-capillary” growth method compatible with full-rotation soft X-ray tomography, providing high-resolution 3D imaging of bacterial cells and their surrounding extracellular matrix during biofilm formation, without drying or fixation steps. These datasets are reconstructed 3D volumes obtained by soft X-ray tomography of WT and ΔtasA mutants, including rescue with TasA protein. The data is organized in individual *.tif files with prefixes as follows: for single-cell suspension WT "Diehl_Biofilm_Bacillus_subtilis_WT_GFP_0h_10797"; for single-cell suspension ΔtasA mutants "Diehl_Biofilm_Bacillus_subtilis_tasA_GFP_0h_10801_"; for WT biofilm formed after 22h "Diehl_Biofilm_Bacillus_subtilis_WT_GFP_10479";for ΔtasA biofilm formed after 22h "Diehl_Biofilm_Bacillus_subtilis_tasA_GFP_10517";for ΔtasA biofilm formed after 44h "Diehl_Biofilm_Bacillus_subtilis_tasA_GFP_ctrl_44h_10815";for ΔtasA biofilm with the addition of TasA protein formed after 44h "Diehl_Biofilm_Bacillus_subtilis_tasA_GFP_rescue_44h_10821" | ||
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