A modified computer-assisted colorimetric microtitre assay (MTT) to assess in vitro radiosensitivity of V79, CaSki, HeLa and WiDr cells
The non-clonogenic MTT assay, based on the reduction of a tetrazolium salt to a purple formazan precipitate by living cells, was modified and a new procedure of analysis is proposed. The colorimetric assay could be performed semiautomatically using microtitre plate reader connected to a personal com...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
1994
|
| In: |
International journal of radiation biology
Year: 1994, Volume: 65, Issue: 2, Pages: 193-201 |
| ISSN: | 1362-3095 |
| DOI: | 10.1080/09553009414550231 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1080/09553009414550231
|
| Author Notes: | M.J. Eble, F.W. Hensley, M. Flentje, A. Schlotz, M. Wannenmacher |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1916564690 | ||
| 003 | DE-627 | ||
| 005 | 20250716230522.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 250206s1994 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1080/09553009414550231 |2 doi | |
| 035 | |a (DE-627)1916564690 | ||
| 035 | |a (DE-599)KXP1916564690 | ||
| 035 | |a (OCoLC)1528018739 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Eble, Michael J. |e VerfasserIn |0 (DE-588)1323335994 |0 (DE-627)1883340942 |4 aut | |
| 245 | 1 | 2 | |a A modified computer-assisted colorimetric microtitre assay (MTT) to assess in vitro radiosensitivity of V79, CaSki, HeLa and WiDr cells |c M.J. Eble, F.W. Hensley, M. Flentje, A. Schlotz, M. Wannenmacher |
| 264 | 1 | |c 1994 | |
| 300 | |a 9 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 06.02.2025 | ||
| 500 | |a Elektronische Reproduktion der Druck-Ausgabe 3. Juli 2009 | ||
| 520 | |a The non-clonogenic MTT assay, based on the reduction of a tetrazolium salt to a purple formazan precipitate by living cells, was modified and a new procedure of analysis is proposed. The colorimetric assay could be performed semiautomatically using microtitre plate reader connected to a personal computer. Data are processed and plotted with a customized program. To ensure that both control and irradiated microtitre plates contain exponentially growing cells at the time of analysis, the calculation of relative survival is based on a series of well-defined cell numbers initially seeded. To make the two endpoints of non-clonogenic and clonogenic assays comparable, i.e. counting of living cells versus counting of colonies, radiation-induced progression delay was incorporated into the calculation. Radiation-induced cell killing and progression delay could be determined in a single analysis, but in an independent way. X-ray survival curves were generated for V79, CaSki, WiDr and HeLa cells using the non-clonogenic and a standard clonogenic assay. Using the linear-quadratic formula, the resulting parameters α,β and the mean inactivation dose were not significantly different. The described assay is a feasible and reproducible technique for determination of cellular survival, which may be able to incorporate progression delay. The equivalence to a clonogenic survival assay could be proven. | ||
| 700 | 1 | |a Hensley, Frank W. |e VerfasserIn |0 (DE-588)1075059895 |0 (DE-627)832910562 |0 (DE-576)278454070 |4 aut | |
| 700 | 1 | |a Flentje, Michael |e VerfasserIn |0 (DE-588)1112561633 |0 (DE-627)866542930 |0 (DE-576)167065122 |4 aut | |
| 700 | 1 | |a Schlotz, Annette |e VerfasserIn |0 (DE-588)1352850125 |0 (DE-627)1913798402 |4 aut | |
| 700 | 1 | |a Wannenmacher, Michael |d 1938-2015 |e VerfasserIn |0 (DE-588)115523588 |0 (DE-627)077305582 |0 (DE-576)289929717 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International journal of radiation biology |d London : Taylor & Francis, 1959 |g 65(1994), 2, Seite 193-201 |h Online-Ressource |w (DE-627)306586541 |w (DE-600)1498203-1 |w (DE-576)081984200 |x 1362-3095 |7 nnas |a A modified computer-assisted colorimetric microtitre assay (MTT) to assess in vitro radiosensitivity of V79, CaSki, HeLa and WiDr cells |
| 773 | 1 | 8 | |g volume:65 |g year:1994 |g number:2 |g pages:193-201 |g extent:9 |a A modified computer-assisted colorimetric microtitre assay (MTT) to assess in vitro radiosensitivity of V79, CaSki, HeLa and WiDr cells |
| 856 | 4 | 0 | |u https://doi.org/10.1080/09553009414550231 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20250206 | ||
| 994 | |a 1994 | ||
| 998 | |g 115523588 |a Wannenmacher, Michael |m 115523588:Wannenmacher, Michael |d 910000 |d 911400 |e 910000PW115523588 |e 911400PW115523588 |k 0/910000/ |k 1/910000/911400/ |p 5 |y j | ||
| 998 | |g 1352850125 |a Schlotz, Annette |m 1352850125:Schlotz, Annette |d 50000 |e 50000PS1352850125 |k 0/50000/ |p 4 | ||
| 998 | |g 1112561633 |a Flentje, Michael |m 1112561633:Flentje, Michael |d 910000 |d 911400 |e 910000PF1112561633 |e 911400PF1112561633 |k 0/910000/ |k 1/910000/911400/ |p 3 | ||
| 998 | |g 1075059895 |a Hensley, Frank W. |m 1075059895:Hensley, Frank W. |d 910000 |d 911400 |e 910000PH1075059895 |e 911400PH1075059895 |k 0/910000/ |k 1/910000/911400/ |p 2 | ||
| 998 | |g 1323335994 |a Eble, Michael J. |m 1323335994:Eble, Michael J. |d 910000 |d 911400 |e 910000PE1323335994 |e 911400PE1323335994 |k 0/910000/ |k 1/910000/911400/ |p 1 |x j | ||
| 999 | |a KXP-PPN1916564690 |e 4661529723 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"origin":[{"dateIssuedDisp":"1994","dateIssuedKey":"1994"}],"id":{"eki":["1916564690"],"doi":["10.1080/09553009414550231"]},"name":{"displayForm":["M.J. Eble, F.W. Hensley, M. Flentje, A. Schlotz, M. Wannenmacher"]},"physDesc":[{"extent":"9 S."}],"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"id":{"issn":["1362-3095"],"zdb":["1498203-1"],"eki":["306586541"]},"origin":[{"dateIssuedKey":"1959","publisher":"Taylor & Francis ; Informa Healthcare","dateIssuedDisp":"1959-","publisherPlace":"London ; London"}],"recId":"306586541","language":["eng"],"note":["Gesehen am 27.10.15"],"type":{"bibl":"periodical","media":"Online-Ressource"},"disp":"A modified computer-assisted colorimetric microtitre assay (MTT) to assess in vitro radiosensitivity of V79, CaSki, HeLa and WiDr cellsInternational journal of radiation biology","part":{"text":"65(1994), 2, Seite 193-201","volume":"65","extent":"9","year":"1994","pages":"193-201","issue":"2"},"pubHistory":["1.1959 -"],"title":[{"title_sort":"International journal of radiation biology","title":"International journal of radiation biology"}]}],"title":[{"title_sort":"modified computer-assisted colorimetric microtitre assay (MTT) to assess in vitro radiosensitivity of V79, CaSki, HeLa and WiDr cells","title":"A modified computer-assisted colorimetric microtitre assay (MTT) to assess in vitro radiosensitivity of V79, CaSki, HeLa and WiDr cells"}],"person":[{"display":"Eble, Michael J.","roleDisplay":"VerfasserIn","role":"aut","family":"Eble","given":"Michael J."},{"given":"Frank W.","family":"Hensley","role":"aut","roleDisplay":"VerfasserIn","display":"Hensley, Frank W."},{"role":"aut","roleDisplay":"VerfasserIn","display":"Flentje, Michael","given":"Michael","family":"Flentje"},{"roleDisplay":"VerfasserIn","display":"Schlotz, Annette","role":"aut","family":"Schlotz","given":"Annette"},{"roleDisplay":"VerfasserIn","display":"Wannenmacher, Michael","role":"aut","family":"Wannenmacher","given":"Michael"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 06.02.2025","Elektronische Reproduktion der Druck-Ausgabe 3. Juli 2009"],"recId":"1916564690","language":["eng"]} | ||
| SRT | |a EBLEMICHAEMODIFIEDCO1994 | ||