On the regulation of the expressed L-type calcium channel by cAMP-dependent phosphorylation
The Ca2+ channel subunits α1C-a and α1C-b were stably expressed in Chinese hamster ovary (CHO) and human embryonic kidney (HEK) 293 cells. The peak Ba2+ current (IBa) of these cells was not affected significantly by internal dialysis with 0.1 mM cAMP-dependent protein kinase inhibitor peptide (mPKI)...
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| Hauptverfasser: | , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
July 1995
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| In: |
Pflügers Archiv
Year: 1995, Jahrgang: 430, Heft: 3, Pages: 340-347 |
| ISSN: | 1432-2013 |
| DOI: | 10.1007/BF00373908 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/BF00373908
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| Verfasserangaben: | Xiangang Zong, Jürgen Schreieck, Gerhard Mehrke, Andera Welling, Angela Schuster, Eva Bosse, Veit Flockerzi, Franz Hofmann |
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| 245 | 1 | 0 | |a On the regulation of the expressed L-type calcium channel by cAMP-dependent phosphorylation |c Xiangang Zong, Jürgen Schreieck, Gerhard Mehrke, Andera Welling, Angela Schuster, Eva Bosse, Veit Flockerzi, Franz Hofmann |
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| 520 | |a The Ca2+ channel subunits α1C-a and α1C-b were stably expressed in Chinese hamster ovary (CHO) and human embryonic kidney (HEK) 293 cells. The peak Ba2+ current (IBa) of these cells was not affected significantly by internal dialysis with 0.1 mM cAMP-dependent protein kinase inhibitor peptide (mPKI), 25 μM cAMP-dependent protein kinase catalytic subunit (PKA), or a combination of 25 μM PKA and 1 μM okadaic acid. The activity of the α1C-b channel subunit expressed stably in HEK 293 cells was depressed by 1 μM H 89 and was not increased by superfusion with 5 μM forskolin plus 20 μM isobutylmethylxanthine (IBMX). The α1C-a·β2·α2/δ complex was transiently expressed in HEK 293 cells; it was inhibited by internal dialysis of the cells with 1 μM H 89, but was not affected by internal dialysis with mPKI, PKA or microcystin. Internal dialysis of cells expressing the α1C-a·β2·α2/δ channel with 10 μM PKA did not induce facilitation after a 150-ms prepulse to +50 mV. The Ca2+ current (ICa) of cardiac myocytes increased threefold during internal dialysis with 5 μM PKA or 25 μM microcystin and during external superfusion with 0.1 μM isoproterenol or 5 μM forskolin plus 50 μM IBMX. These results indicate that the L-type Ca2+ channel expressed is not modulated by cAMP-dependent phosphorylation to the same extent as in native cardiac myocytes. | ||
| 650 | 4 | |a cAMP-dependent regulation of calcium channels | |
| 650 | 4 | |a CHO cells | |
| 650 | 4 | |a HEK 293 cells | |
| 650 | 4 | |a L-Type calcium channels | |
| 650 | 4 | |a Transient and stable expression of calcium channels | |
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