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Alzheimer’s disease linked Aβ42 exerts product feedback inhibition on γ-secretase impairing downstream cell signaling

Amyloid β (Aβ) peptides accumulating in the brain are proposed to trigger Alzheimer’s disease (AD). However, molecular cascades underlying their toxicity are poorly defined. Here, we explored a novel hypothesis for Aβ42 toxicity that arises from its proven affinity for γ-secretases. We hypothesized...

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Hauptverfasser: Zoltowska, Katarzyna Marta (VerfasserIn) , Das, Utpal (VerfasserIn) , Lismont, Sam (VerfasserIn) , Enzlein, Thomas (VerfasserIn) , Maesako, Masato (VerfasserIn) , Houser, Mei CQ (VerfasserIn) , Franco, Maria Luisa (VerfasserIn) , Özcan, Burcu (VerfasserIn) , Gomes Moreira, Diana (VerfasserIn) , Karachentsev, Dmitry (VerfasserIn) , Becker, Ann (VerfasserIn) , Hopf, Carsten (VerfasserIn) , Vilar, Marçal (VerfasserIn) , Berezovska, Oksana (VerfasserIn) , Mobley, William (VerfasserIn) , Chávez-Gutiérrez, Lucía (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 19 July 2024
In: eLife
Year: 2024, Jahrgang: 12, Pages: 1-34
ISSN:2050-084X
DOI:10.7554/eLife.90690
Online-Zugang:Verlag, kostenfrei, Volltext: https://doi.org/10.7554/eLife.90690
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Verfasserangaben:Katarzyna Marta Zoltowska, Utpal Das, Sam Lismont, Thomas Enzlein, Masato Maesako, Mei CQ Houser, Maria Luisa Franco, Burcu Özcan, Diana Gomes Moreira, Dmitry Karachentsev, Ann Becker, Carsten Hopf, Marçal Vilar, Oksana Berezovska, William Mobley, Lucía Chávez-Gutiérrez
Beschreibung
Zusammenfassung:Amyloid β (Aβ) peptides accumulating in the brain are proposed to trigger Alzheimer’s disease (AD). However, molecular cascades underlying their toxicity are poorly defined. Here, we explored a novel hypothesis for Aβ42 toxicity that arises from its proven affinity for γ-secretases. We hypothesized that the reported increases in Aβ42, particularly in the endolysosomal compartment, promote the establishment of a product feedback inhibitory mechanism on γ-secretases, and thereby impair downstream signaling events. We conducted kinetic analyses of γ-secretase activity in cell-free systems in the presence of Aβ, as well as cell-based and ex vivo assays in neuronal cell lines, neurons, and brain synaptosomes to assess the impact of Aβ on γ-secretases. We show that human Aβ42 peptides, but neither murine Aβ42 nor human Aβ17-42 (p3), inhibit γ-secretases and trigger accumulation of unprocessed substrates in neurons, including C-terminal fragments (CTFs) of APP, p75, and pan-cadherin. Moreover, Aβ42 treatment dysregulated cellular homeostasis, as shown by the induction of p75-dependent neuronal death in two distinct cellular systems. Our findings raise the possibility that pathological elevations in Aβ42 contribute to cellular toxicity via the γ-secretase inhibition, and provide a novel conceptual framework to address Aβ toxicity in the context of γ-secretase-dependent homeostatic signaling.
Beschreibung:Gesehen am 24.02.2024
Beschreibung:Online Resource
ISSN:2050-084X
DOI:10.7554/eLife.90690

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