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A perfusion-independent high-throughput method to isolate liver sinusoidal endothelial cells

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Liver sinusoidal endothelial cells (LSECs) critically regulate homeostatic liver function and liver pathogenesis. However, the isolation of LSECs remains a major technological bottleneck in studying molecular mechanisms governing LSEC functions. Current techniques to isolate LSECs, relying on perfus...

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Main Authors: Babin-Ebell Gonçalves, Anna (Author) , Mao, Yifang (Author) , Baljkas, Tinja (Author) , Wiedmann, Felix Tobias (Author) , Eis, Larissa (Author) , Pilz, Franziska (Author) , Winkler, Manuel (Author) , Kürschner, Sina Wietje (Author) , Hoffarth, Marlene (Author) , Funaya, Charlotta (Author) , Shahidi, Réza (Author) , Géraud, Cyrill (Author) , Wu, Chi-Chung (Author) , Schmidt, Constanze (Author) , Goerdt, Sergij (Author) , Reiners-Koch, Philipp-Sebastian (Author) , Singhal, Mahak (Author)
Format: Article (Journal)
Language:English
Published: 8. Januar 2025
In: Communications biology
Year: 2025, Volume: 8, Issue: 1, Pages: 1-10
ISSN:2399-3642
DOI:10.1038/s42003-025-07458-5
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s42003-025-07458-5
Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s42003-025-07458-5
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Author Notes:Anna Babin-Ebell Gonçalves, Yifang Mao, Tinja Baljkas, Felix Wiedmann, Larissa Eis, Franziska Pilz, Manuel Winkler, Sina W. Kürschner-Zacharias, Marlene Hoffarth, Charlotta Funaya, Réza Shahidi, Cyrill Géraud, Chi-Chung Wu, Constanze Schmidt, Sergij Goerdt, Philipp-Sebastian Reiners-Koch & Mahak Singhal
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A perfusion-independent high-throughput method to isolate liver sinusoidal endothelial cells by Babin-Ebell Gonçalves, Anna (Author) , Mao, Yifang (Author) , Baljkas, Tinja (Author) , Wiedmann, Felix Tobias (Author) , Pilz, Franziska (Author) , Winkler, Manuel (Author) , Kürschner, Sina Wietje (Author) , Hoffarth, Marlene (Author) , Schmidt, Constanze (Author) , Goerdt, Sergij (Author) , Reiners-Koch, Philipp-Sebastian (Author) , Singhal, Mahak (Author) ,


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