Cover Image

The conserved HIV-1 spacer peptide 2 triggers matrix lattice maturation

The virus particles of human immunodeficiency virus type 1 (HIV-1) are released in an immature, non-infectious form. Proteolytic cleavage of the main structural polyprotein Gag into functional domains induces rearrangement into mature, infectious virions. In immature virus particles, the Gag membran...

Full description

Saved in:
Bibliographic Details
Main Authors: Stacey, James C. V. (Author) , Hrebík, Dominik (Author) , Nand, Elizabeth (Author) , Shetty, Snehith Dyavari (Author) , Qu, Kun (Author) , Boicu, Marius (Author) , Anders-Ößwein, Maria (Author) , Uchil, Pradeep D. (Author) , Dick, Robert A. (Author) , Mothes, Walther (Author) , Kräusslich, Hans-Georg (Author) , Müller, Barbara (Author) , Briggs, John A. G. (Author)
Format: Article (Journal)
Language:English
Published: 26 February 2025
In: Nature
Year: 2025, Volume: 640, Issue: 8057, Pages: 258-264, [1-17]
ISSN:1476-4687
DOI:10.1038/s41586-025-08624-9
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41586-025-08624-9
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s41586-025-08624-9
Get full text
Author Notes:James C. V. Stacey, Dominik Hrebík, Elizabeth Nand, Snehith Dyavari Shetty, Kun Qu, Marius Boicu, Maria Anders-Össwein, Pradeep D. Uchil, Robert A. Dick, Walther Mothes, Hans-Georg Kräusslich, Barbara Müller, John A. G. Briggs

MARC

LEADER 00000caa a2200000 c 4500
001 1924010992
003 DE-627
005 20250717012259.0
007 cr uuu---uuuuu
008 250430s2025 xx |||||o 00| ||eng c
024 7 |a 10.1038/s41586-025-08624-9  |2 doi 
035 |a (DE-627)1924010992 
035 |a (DE-599)KXP1924010992 
035 |a (OCoLC)1528045023 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Stacey, James C. V.  |e VerfasserIn  |0 (DE-588)1364151472  |0 (DE-627)1924010445  |4 aut 
245 1 4 |a The conserved HIV-1 spacer peptide 2 triggers matrix lattice maturation  |c James C. V. Stacey, Dominik Hrebík, Elizabeth Nand, Snehith Dyavari Shetty, Kun Qu, Marius Boicu, Maria Anders-Össwein, Pradeep D. Uchil, Robert A. Dick, Walther Mothes, Hans-Georg Kräusslich, Barbara Müller, John A. G. Briggs 
264 1 |c 26 February 2025 
300 |a 34 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 30.04.2025 
520 |a The virus particles of human immunodeficiency virus type 1 (HIV-1) are released in an immature, non-infectious form. Proteolytic cleavage of the main structural polyprotein Gag into functional domains induces rearrangement into mature, infectious virions. In immature virus particles, the Gag membrane-binding domain, MA, forms a hexameric protein lattice that undergoes structural transition, following cleavage, into a distinct, mature MA lattice1. The mechanism of MA lattice maturation is unknown. Here we show that released spacer peptide 2 (SP2), a conserved peptide of unknown function situated about 300 residues downstream of MA, binds MA to induce structural maturation. By high-resolution in-virus structure determination of MA, we show that MA does not bind lipid into a side pocket as previously thought1, but instead binds SP2 as an integral part of the protein-protein interfaces that stabilize the mature lattice. Analysis of Gag cleavage site mutants showed that SP2 release is required for MA maturation, and we demonstrate that SP2 is sufficient to induce maturation of purified MA on lipid monolayers in vitro. SP2-triggered MA maturation correlated with faster fusion of virus with target cells. Our results reveal a new, unexpected interaction between two HIV-1 components, provide a high-resolution structure of mature MA, establish the trigger of MA structural maturation and assign function to the SP2 peptide. 
650 4 |a Cryoelectron microscopy 
650 4 |a Retrovirus 
650 4 |a Virus structures 
700 1 |a Hrebík, Dominik  |e VerfasserIn  |4 aut 
700 1 |a Nand, Elizabeth  |e VerfasserIn  |4 aut 
700 1 |a Shetty, Snehith Dyavari  |e VerfasserIn  |4 aut 
700 1 |8 1\p  |a Qu, Kun  |e VerfasserIn  |0 (DE-588)1210450992  |0 (DE-627)1698486928  |4 aut 
700 1 |a Boicu, Marius  |e VerfasserIn  |4 aut 
700 1 |a Anders-Ößwein, Maria  |e VerfasserIn  |0 (DE-588)1027600743  |0 (DE-627)729456323  |0 (DE-576)373448627  |4 aut 
700 1 |a Uchil, Pradeep D.  |e VerfasserIn  |4 aut 
700 1 |a Dick, Robert A.  |e VerfasserIn  |4 aut 
700 1 |a Mothes, Walther  |e VerfasserIn  |4 aut 
700 1 |a Kräusslich, Hans-Georg  |d 1958-  |e VerfasserIn  |0 (DE-588)1027601782  |0 (DE-627)729459004  |0 (DE-576)373450524  |4 aut 
700 1 |a Müller, Barbara  |d 1961-  |e VerfasserIn  |0 (DE-588)1028058667  |0 (DE-627)730238385  |0 (DE-576)375540024  |4 aut 
700 1 |8 2\p  |a Briggs, John A. G.  |e VerfasserIn  |0 (DE-588)1072596881  |0 (DE-627)827881592  |0 (DE-576)434097349  |4 aut 
773 0 8 |i Enthalten in  |t Nature  |d London [u.a.] : Nature Publ. Group, 1869  |g 640(2025), 8057, Seite 258-264, [1-17]  |h Online-Ressource  |w (DE-627)240151402  |w (DE-600)1413423-8  |w (DE-576)079718426  |x 1476-4687  |7 nnas  |a The conserved HIV-1 spacer peptide 2 triggers matrix lattice maturation 
773 1 8 |g volume:640  |g year:2025  |g number:8057  |g pages:258-264, [1-17]  |g extent:34  |a The conserved HIV-1 spacer peptide 2 triggers matrix lattice maturation 
856 4 0 |u https://doi.org/10.1038/s41586-025-08624-9  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://www.nature.com/articles/s41586-025-08624-9  |x Verlag  |z lizenzpflichtig  |3 Volltext 
883 |8 1\p  |a cgwrk  |d 20250701  |q DE-101  |u https://d-nb.info/provenance/plan#cgwrk 
883 |8 2\p  |a cgwrk  |d 20250701  |q DE-101  |u https://d-nb.info/provenance/plan#cgwrk 
951 |a AR 
992 |a 20250430 
994 |a 2025 
998 |g 1028058667  |a Müller, Barbara  |m 1028058667:Müller, Barbara  |d 910000  |d 911700  |d 50000  |e 910000PM1028058667  |e 911700PM1028058667  |e 50000PM1028058667  |k 0/910000/  |k 1/910000/911700/  |k 0/50000/  |p 12 
998 |g 1027601782  |a Kräusslich, Hans-Georg  |m 1027601782:Kräusslich, Hans-Georg  |d 910000  |d 911700  |e 910000PK1027601782  |e 911700PK1027601782  |k 0/910000/  |k 1/910000/911700/  |p 11 
998 |g 1027600743  |a Anders-Ößwein, Maria  |m 1027600743:Anders-Ößwein, Maria  |d 50000  |e 50000PA1027600743  |k 0/50000/  |p 7 
999 |a KXP-PPN1924010992  |e 4717991877 
BIB |a Y 
SER |a newspaper 
JSO |a {"relHost":[{"id":{"issn":["1476-4687"],"eki":["240151402"],"zdb":["1413423-8"]},"part":{"pages":"258-264, [1-17]","extent":"34","issue":"8057","text":"640(2025), 8057, Seite 258-264, [1-17]","volume":"640","year":"2025"},"physDesc":[{"extent":"Online-Ressource"}],"disp":"The conserved HIV-1 spacer peptide 2 triggers matrix lattice maturationNature","recId":"240151402","language":["eng"],"titleAlt":[{"title":"Nature <London>"},{"title":"Nature"}],"pubHistory":["1.1869/70 - ; auch mit durchgehender Nr.-Zählung"],"title":[{"title":"Nature","title_sort":"Nature","subtitle":"international weekly journal of science"}],"note":["Gesehen am 19.02.2019"],"origin":[{"publisherPlace":"London [u.a.] ; London","dateIssuedKey":"1869","publisher":"Nature Publ. Group ; Macmillan","dateIssuedDisp":"1869-"}],"type":{"bibl":"newspaper","media":"Online-Ressource"}}],"person":[{"display":"Stacey, James C. V.","roleDisplay":"VerfasserIn","role":"aut","family":"Stacey","given":"James C. V."},{"roleDisplay":"VerfasserIn","role":"aut","family":"Hrebík","given":"Dominik","display":"Hrebík, Dominik"},{"role":"aut","roleDisplay":"VerfasserIn","given":"Elizabeth","family":"Nand","display":"Nand, Elizabeth"},{"role":"aut","roleDisplay":"VerfasserIn","given":"Snehith Dyavari","family":"Shetty","display":"Shetty, Snehith Dyavari"},{"roleDisplay":"VerfasserIn","role":"aut","family":"Qu","given":"Kun","display":"Qu, Kun"},{"family":"Boicu","given":"Marius","roleDisplay":"VerfasserIn","role":"aut","display":"Boicu, Marius"},{"role":"aut","roleDisplay":"VerfasserIn","family":"Anders-Ößwein","given":"Maria","display":"Anders-Ößwein, Maria"},{"display":"Uchil, Pradeep D.","given":"Pradeep D.","family":"Uchil","role":"aut","roleDisplay":"VerfasserIn"},{"roleDisplay":"VerfasserIn","role":"aut","family":"Dick","given":"Robert A.","display":"Dick, Robert A."},{"role":"aut","roleDisplay":"VerfasserIn","family":"Mothes","given":"Walther","display":"Mothes, Walther"},{"family":"Kräusslich","given":"Hans-Georg","role":"aut","roleDisplay":"VerfasserIn","display":"Kräusslich, Hans-Georg"},{"display":"Müller, Barbara","family":"Müller","given":"Barbara","role":"aut","roleDisplay":"VerfasserIn"},{"roleDisplay":"VerfasserIn","role":"aut","family":"Briggs","given":"John A. G.","display":"Briggs, John A. G."}],"id":{"eki":["1924010992"],"doi":["10.1038/s41586-025-08624-9"]},"note":["Gesehen am 30.04.2025"],"type":{"media":"Online-Ressource","bibl":"article-newspaper"},"origin":[{"dateIssuedKey":"2025","dateIssuedDisp":"26 February 2025"}],"language":["eng"],"recId":"1924010992","title":[{"title":"The conserved HIV-1 spacer peptide 2 triggers matrix lattice maturation","title_sort":"conserved HIV-1 spacer peptide 2 triggers matrix lattice maturation"}],"name":{"displayForm":["James C. V. Stacey, Dominik Hrebík, Elizabeth Nand, Snehith Dyavari Shetty, Kun Qu, Marius Boicu, Maria Anders-Össwein, Pradeep D. Uchil, Robert A. Dick, Walther Mothes, Hans-Georg Kräusslich, Barbara Müller, John A. G. Briggs"]},"physDesc":[{"extent":"34 S."}]} 
SRT |a STACEYJAMECONSERVEDH2620 

Similar Items