Cover Image

Role of (Na+)i and (Ca2+)i in nicotine-induced norepinephrine release from bovine adrenal chromaffin cells

Intracellular free sodium ([Na+]i) and calcium ([Ca2+]i) concentrations were determined by sodium-binding benzofuran isophthalate (SBFI) and fura 2 microfluorimetry, respectively, in bovine adrenal chromaffin cells (BCC). Validation of SBFI microfluorimetry by in vitro and in vivo calibration reveal...

Full description

Saved in:
Bibliographic Details
Main Authors: Gerber, Stefan Horst (Author) , Haunstetter, Armin (Author) , Krüger, Carsten (Author) , Kaufmann, Alexander (Author) , Nobiling, Rainer (Author) , Haass, Markus (Author)
Format: Article (Journal)
Language:English
Published: 1 September 1995
In: American journal of physiology. Cell physiology
Year: 1995, Volume: 269, Issue: 3, Pages: C572-C581
ISSN:1522-1563
DOI:10.1152/ajpcell.1995.269.3.C572
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1152/ajpcell.1995.269.3.C572
Verlag, lizenzpflichtig, Volltext: https://journals.physiology.org/doi/abs/10.1152/ajpcell.1995.269.3.C572
Get full text
Author Notes:Stefan H. Gerber, Armin Haunstetter, Carsten Krüger, Alexander Kaufmann, Rainer Nobiling, and Markus Haass

MARC

LEADER 00000caa a2200000 c 4500
001 1925306550
003 DE-627
005 20250717013205.0
007 cr uuu---uuuuu
008 250512s1995 xx |||||o 00| ||eng c
024 7 |a 10.1152/ajpcell.1995.269.3.C572  |2 doi 
035 |a (DE-627)1925306550 
035 |a (DE-599)KXP1925306550 
035 |a (OCoLC)1528045296 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Gerber, Stefan Horst  |d 1970-  |e VerfasserIn  |0 (DE-588)118194178  |0 (DE-627)694752037  |0 (DE-576)291762611  |4 aut 
245 1 0 |a Role of (Na+)i and (Ca2+)i in nicotine-induced norepinephrine release from bovine adrenal chromaffin cells  |c Stefan H. Gerber, Armin Haunstetter, Carsten Krüger, Alexander Kaufmann, Rainer Nobiling, and Markus Haass 
264 1 |c 1 September 1995 
300 |a 10 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 12.05.2025 
520 |a Intracellular free sodium ([Na+]i) and calcium ([Ca2+]i) concentrations were determined by sodium-binding benzofuran isophthalate (SBFI) and fura 2 microfluorimetry, respectively, in bovine adrenal chromaffin cells (BCC). Validation of SBFI microfluorimetry by in vitro and in vivo calibration revealed a reliable assessment of [Na+]i within a range of 1-30 mM in single BCC. Nicotine (0.1-10 microM) induced concentration-dependent increases of both [Na+]i (from 3.3 +/- 0.1 to 25.6 +/- 0.4 mM, n = 76, P < 0.001) and [Ca2+]i (from 64 +/- 1 to 467 +/- 16 nM, n = 87, P < 0.001), which were accompanied by an increase in [3H]norepinephrine (NE) release. Consistent with an exocytotic release mechanism, nicotine-induced increments of [Ca2+]i and [3H]NE release were reduced under calcium-free conditions and by gadolinium chloride (40 microM), whereas [Na+]i was not affected. In contrast, a parallel attenuation of nicotine-evoked changes in [Na+]i, [Ca2+]i, and [3H]NE release was observed during reduction of the extracellular sodium concentration. The nicotine-evoked responses were neutralized by the nicotinic receptor antagonist hexamethonium (100 microM) but not by blockade of voltage-dependent sodium channels (1 microM tetrodotoxin). In conclusion, the nicotine-induced exocytotic release of [3H]NE is triggered by an increase in [Ca2+]i, which is facilitated by sodium influx through the nicotinic receptor ionophore. 
700 1 |a Haunstetter, Armin  |e VerfasserIn  |0 (DE-588)1052546978  |0 (DE-627)788604570  |0 (DE-576)408318139  |4 aut 
700 1 |a Krüger, Carsten  |e VerfasserIn  |0 (DE-588)1076636705  |0 (DE-627)835137910  |0 (DE-576)445491493  |4 aut 
700 1 |a Kaufmann, Alexander  |e VerfasserIn  |4 aut 
700 1 |a Nobiling, Rainer  |e VerfasserIn  |0 (DE-588)107676990X  |0 (DE-627)835318249  |0 (DE-576)179265369  |4 aut 
700 1 |a Haass, Markus  |e VerfasserIn  |0 (DE-588)140599282  |0 (DE-627)620224517  |0 (DE-576)319037940  |4 aut 
773 0 8 |i Enthalten in  |t American journal of physiology. Cell physiology  |d Bethesda, Md. : American Physiological Society, 1977  |g 269(1995), 3, Seite C572-C581  |h Online-Ressource  |w (DE-627)270426523  |w (DE-600)1477334-X  |w (DE-576)079718841  |x 1522-1563  |7 nnas  |a Role of (Na+)i and (Ca2+)i in nicotine-induced norepinephrine release from bovine adrenal chromaffin cells 
773 1 8 |g volume:269  |g year:1995  |g number:3  |g pages:C572-C581  |g extent:10  |a Role of (Na+)i and (Ca2+)i in nicotine-induced norepinephrine release from bovine adrenal chromaffin cells 
856 4 0 |u https://doi.org/10.1152/ajpcell.1995.269.3.C572  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://journals.physiology.org/doi/abs/10.1152/ajpcell.1995.269.3.C572  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20250512 
994 |a 1995 
998 |g 140599282  |a Haass, Markus  |m 140599282:Haass, Markus  |d 50000  |e 50000PH140599282  |k 0/50000/  |p 6  |y j 
998 |g 107676990X  |a Nobiling, Rainer  |m 107676990X:Nobiling, Rainer  |d 910000  |d 910200  |e 910000PN107676990X  |e 910200PN107676990X  |k 0/910000/  |k 1/910000/910200/  |p 5 
998 |g 1076636705  |a Krüger, Carsten  |m 1076636705:Krüger, Carsten  |d 910000  |d 910100  |e 910000PK1076636705  |e 910100PK1076636705  |k 0/910000/  |k 1/910000/910100/  |p 3 
998 |g 1052546978  |a Haunstetter, Armin  |m 1052546978:Haunstetter, Armin  |d 910000  |d 910100  |e 910000PH1052546978  |e 910100PH1052546978  |k 0/910000/  |k 1/910000/910100/  |p 2 
998 |g 118194178  |a Gerber, Stefan Horst  |m 118194178:Gerber, Stefan Horst  |d 50000  |e 50000PG118194178  |k 0/50000/  |p 1  |x j 
999 |a KXP-PPN1925306550  |e 4723456341 
BIB |a Y 
SER |a journal 
JSO |a {"physDesc":[{"extent":"10 S."}],"relHost":[{"id":{"eki":["270426523"],"zdb":["1477334-X"],"issn":["1522-1563"]},"origin":[{"dateIssuedDisp":"1977-","publisher":"American Physiological Society","dateIssuedKey":"1977","publisherPlace":"Bethesda, Md."}],"name":{"displayForm":["publ. by the American Physiological Society"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"American journal of physiology","title":"American journal of physiology","partname":"Cell physiology"}],"part":{"text":"269(1995), 3, Seite C572-C581","volume":"269","extent":"10","year":"1995","issue":"3","pages":"C572-C581"},"titleAlt":[{"title":"American journal of physiology / Cell physiology"},{"title":"Cell physiology"}],"pubHistory":["232.1977 -"],"recId":"270426523","language":["eng"],"corporate":[{"role":"isb","display":"American Physiological Society","roleDisplay":"Herausgebendes Organ"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Role of (Na+)i and (Ca2+)i in nicotine-induced norepinephrine release from bovine adrenal chromaffin cellsAmerican journal of physiology. Cell physiology","note":["Gesehen am 05.02.20"]}],"origin":[{"dateIssuedKey":"1995","dateIssuedDisp":"1 September 1995"}],"id":{"doi":["10.1152/ajpcell.1995.269.3.C572"],"eki":["1925306550"]},"name":{"displayForm":["Stefan H. Gerber, Armin Haunstetter, Carsten Krüger, Alexander Kaufmann, Rainer Nobiling, and Markus Haass"]},"note":["Gesehen am 12.05.2025"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"recId":"1925306550","title":[{"title_sort":"Role of (Na+)i and (Ca2+)i in nicotine-induced norepinephrine release from bovine adrenal chromaffin cells","title":"Role of (Na+)i and (Ca2+)i in nicotine-induced norepinephrine release from bovine adrenal chromaffin cells"}],"person":[{"given":"Stefan Horst","family":"Gerber","role":"aut","roleDisplay":"VerfasserIn","display":"Gerber, Stefan Horst"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Haunstetter, Armin","given":"Armin","family":"Haunstetter"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Krüger, Carsten","given":"Carsten","family":"Krüger"},{"family":"Kaufmann","given":"Alexander","roleDisplay":"VerfasserIn","display":"Kaufmann, Alexander","role":"aut"},{"role":"aut","display":"Nobiling, Rainer","roleDisplay":"VerfasserIn","given":"Rainer","family":"Nobiling"},{"given":"Markus","family":"Haass","role":"aut","display":"Haass, Markus","roleDisplay":"VerfasserIn"}]} 
SRT |a GERBERSTEFROLEOFNAIA1199 

Similar Items