Aging platelets shift their hemostatic properties to inflammatory functions: platelets and thrombopoiesis
Platelets are crucial players in hemostasis and thrombosis but also contribute to immune regulation and host defense, using different receptors, signaling pathways, and effector functions, respectively. Whether distinct subsets of platelets specialize in these diverse tasks is insufficiently underst...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
April 3 2025
|
| In: |
Blood
Year: 2025, Jahrgang: 145, Heft: 14, Pages: 1568-1582 |
| ISSN: | 1528-0020 |
| DOI: | 10.1182/blood.2024024901 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1182/blood.2024024901
|
| Verfasserangaben: | Afra Anjum, Magdalena Mader, Shaan Mahameed, Abhinaya Muraly, Frederik Denorme, Fabian P. Kliem, Dario Rossaro, Sezer Agköl, Lea Di Fina, Maité Mulkers, Lisa Laun, Lukas Li, Nadja Kupper, Keyang Yue, Marie-Louise Hoffknecht, Anastassia Akhalkatsi, Quentin Loew, Joachim Pircher, Raphael Escaig, Erwin Strasser, Christian Wichmann, Kami Pekayvaz, Bernhard Nieswandt, Christian Schulz, Maria S. Robles, Rainer Kaiser, Steffen Massberg, Robert Campbell, and Leo Nicolai |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1926695135 | ||
| 003 | DE-627 | ||
| 005 | 20250913141538.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 250527s2025 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1182/blood.2024024901 |2 doi | |
| 035 | |a (DE-627)1926695135 | ||
| 035 | |a (DE-599)KXP1926695135 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Anjum, Afra |e VerfasserIn |0 (DE-588)1366903536 |0 (DE-627)1926695704 |4 aut | |
| 245 | 1 | 0 | |a Aging platelets shift their hemostatic properties to inflammatory functions |b platelets and thrombopoiesis |c Afra Anjum, Magdalena Mader, Shaan Mahameed, Abhinaya Muraly, Frederik Denorme, Fabian P. Kliem, Dario Rossaro, Sezer Agköl, Lea Di Fina, Maité Mulkers, Lisa Laun, Lukas Li, Nadja Kupper, Keyang Yue, Marie-Louise Hoffknecht, Anastassia Akhalkatsi, Quentin Loew, Joachim Pircher, Raphael Escaig, Erwin Strasser, Christian Wichmann, Kami Pekayvaz, Bernhard Nieswandt, Christian Schulz, Maria S. Robles, Rainer Kaiser, Steffen Massberg, Robert Campbell, and Leo Nicolai |
| 264 | 1 | |c April 3 2025 | |
| 300 | |b Illustrationen, Diagramme | ||
| 300 | |a 15 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 27.05.2025 | ||
| 520 | |a Platelets are crucial players in hemostasis and thrombosis but also contribute to immune regulation and host defense, using different receptors, signaling pathways, and effector functions, respectively. Whether distinct subsets of platelets specialize in these diverse tasks is insufficiently understood. Here, we used a pulse-labeling method in Mus musculus models for tracking in vivo platelet aging and its functional implications. Using in vitro and in vivo assays, we reveal that young, reticulated platelets show heightened responses in the setting of clot formation, with corresponding, increased responses to agonists, adhesion, and retractile function. Unexpectedly, aged platelets lose their hemostatic proficiency but are more prone to react to inflammatory challenge: compared with reticulated platelets, this cohort was more likely to form platelet-leukocyte aggregates and showed increased adhesion to neutrophils in vitro, as well as enhanced bactericidal function. In vivo, this was reflected in increased pulmonary recruitment of aged platelets in an acute lung injury model. Proteomic analyses confirmed the upregulation of immune pathways in this cohort, including enhanced procoagulant function. In mouse models of prolonged platelet half-life, this resulted in increased pulmonary leukocyte infiltration and inflammation upon acute lung injury. Similarly, human platelet concentrates decreased their hemostatic function and elevated their putative immunomodulatory potential in vitro over time, and in a mouse model of platelet transfusion, aged platelet concentrates resulted in augmented inflammation. In summary, we show that platelets exhibit age-dependent phenotypic shifts, allowing them to fulfill their diverse tasks in the vasculature. Because functional alterations of aging platelets extend to platelet concentrates, this may hold important implications for transfusion medicine. | ||
| 700 | 1 | |a Mader, Magdalena |e VerfasserIn |4 aut | |
| 700 | 1 | |a Mahameed, Shaan |e VerfasserIn |4 aut | |
| 700 | 1 | |a Muraly, Abhinaya |e VerfasserIn |4 aut | |
| 700 | 1 | |a Denorme, Frederik |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kliem, Fabian P. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Rossaro, Dario |e VerfasserIn |4 aut | |
| 700 | 1 | |a Agköl, Sezer |e VerfasserIn |4 aut | |
| 700 | 1 | |a Di Fina, Lea |e VerfasserIn |4 aut | |
| 700 | 1 | |a Mulkers, Maité |e VerfasserIn |4 aut | |
| 700 | 1 | |a Laun, Lisa |e VerfasserIn |4 aut | |
| 700 | 1 | |a Li, Lukas |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kupper, Nadja |e VerfasserIn |4 aut | |
| 700 | 1 | |a Yue, Keyang |e VerfasserIn |4 aut | |
| 700 | 1 | |a Hoffknecht, Marie-Louise |e VerfasserIn |4 aut | |
| 700 | 1 | |a Akhalkatsi, Anastassia |e VerfasserIn |4 aut | |
| 700 | 1 | |a Loew, Quentin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Pircher, Joachim |e VerfasserIn |4 aut | |
| 700 | 1 | |a Escaig, Raphael |e VerfasserIn |4 aut | |
| 700 | 1 | |a Strasser, Erwin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wichmann, Christian |e VerfasserIn |4 aut | |
| 700 | 1 | |a Pekayvaz, Kami |e VerfasserIn |4 aut | |
| 700 | 1 | |a Nieswandt, Bernhard |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schulz, Christian |d 1976- |e VerfasserIn |0 (DE-588)129423122 |0 (DE-627)396080618 |0 (DE-576)29765280X |4 aut | |
| 700 | 1 | |a Robles, Maria S. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kaiser, Rainer |e VerfasserIn |4 aut | |
| 700 | 1 | |a Massberg, Steffen |e VerfasserIn |4 aut | |
| 700 | 1 | |a Campbell, Robert |e VerfasserIn |4 aut | |
| 700 | 1 | |a Nicolai, Leo |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Blood |d Washington, DC : American Society of Hematology, 1946 |g 145(2025), 14 vom: Apr., Seite 1568-1582 |h Online-Ressource |w (DE-627)266886647 |w (DE-600)1468538-3 |w (DE-576)075961938 |x 1528-0020 |7 nnas |a Aging platelets shift their hemostatic properties to inflammatory functions platelets and thrombopoiesis |
| 773 | 1 | 8 | |g volume:145 |g year:2025 |g number:14 |g month:04 |g pages:1568-1582 |g extent:15 |a Aging platelets shift their hemostatic properties to inflammatory functions platelets and thrombopoiesis |
| 787 | 0 | 8 | |i Ergänzung |a Gutiérrez, Laura |t “When I’m 64”: functional shift of aging platelets, in: Blood |d 2025 |g Vol. 145(14), 1447-1449 |o 10.1182/blood.2024027996 |
| 856 | 4 | 0 | |u https://doi.org/10.1182/blood.2024024901 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20250527 | ||
| 993 | |a Article | ||
| 994 | |a 2025 | ||
| 998 | |g 129423122 |a Schulz, Christian |m 129423122:Schulz, Christian |d 60000 |d 65400 |e 60000PS129423122 |e 65400PS129423122 |k 0/60000/ |k 1/60000/65400/ |p 15 | ||
| 999 | |a KXP-PPN1926695135 |e 4728271838 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"physDesc":[{"noteIll":"Illustrationen, Diagramme","extent":"15 S."}],"relHost":[{"title":[{"title_sort":"Blood","title":"Blood","subtitle":"journal of the American Society of Hematology"}],"note":["Gesehen am 21.04.2023"],"disp":"Aging platelets shift their hemostatic properties to inflammatory functions platelets and thrombopoiesisBlood","type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"266886647","corporate":[{"roleDisplay":"Herausgebendes Organ","display":"American Society of Hematology","role":"isb"}],"language":["eng"],"pubHistory":["1.1946 -"],"part":{"extent":"15","text":"145(2025), 14 vom: Apr., Seite 1568-1582","volume":"145","issue":"14","pages":"1568-1582","year":"2025"},"titleAlt":[{"title":"Blood online"}],"origin":[{"dateIssuedDisp":"1946-","publisher":"American Society of Hematology ; Saunders ; HighWire Press","dateIssuedKey":"1946","publisherPlace":"Washington, DC ; Philadelphia, Pa. ; Stanford, Calif."}],"id":{"issn":["1528-0020"],"eki":["266886647"],"zdb":["1468538-3"]},"physDesc":[{"extent":"Online-Ressource"}]}],"name":{"displayForm":["Afra Anjum, Magdalena Mader, Shaan Mahameed, Abhinaya Muraly, Frederik Denorme, Fabian P. Kliem, Dario Rossaro, Sezer Agköl, Lea Di Fina, Maité Mulkers, Lisa Laun, Lukas Li, Nadja Kupper, Keyang Yue, Marie-Louise Hoffknecht, Anastassia Akhalkatsi, Quentin Loew, Joachim Pircher, Raphael Escaig, Erwin Strasser, Christian Wichmann, Kami Pekayvaz, Bernhard Nieswandt, Christian Schulz, Maria S. Robles, Rainer Kaiser, Steffen Massberg, Robert Campbell, and Leo Nicolai"]},"origin":[{"dateIssuedDisp":"April 3 2025","dateIssuedKey":"2025"}],"id":{"doi":["10.1182/blood.2024024901"],"eki":["1926695135"]},"note":["Gesehen am 27.05.2025"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"recId":"1926695135","language":["eng"],"person":[{"family":"Anjum","given":"Afra","roleDisplay":"VerfasserIn","display":"Anjum, Afra","role":"aut"},{"given":"Magdalena","family":"Mader","role":"aut","roleDisplay":"VerfasserIn","display":"Mader, Magdalena"},{"family":"Mahameed","given":"Shaan","display":"Mahameed, Shaan","roleDisplay":"VerfasserIn","role":"aut"},{"given":"Abhinaya","family":"Muraly","role":"aut","display":"Muraly, Abhinaya","roleDisplay":"VerfasserIn"},{"given":"Frederik","family":"Denorme","role":"aut","display":"Denorme, Frederik","roleDisplay":"VerfasserIn"},{"display":"Kliem, Fabian P.","roleDisplay":"VerfasserIn","role":"aut","family":"Kliem","given":"Fabian P."},{"role":"aut","roleDisplay":"VerfasserIn","display":"Rossaro, Dario","given":"Dario","family":"Rossaro"},{"roleDisplay":"VerfasserIn","display":"Agköl, Sezer","role":"aut","family":"Agköl","given":"Sezer"},{"given":"Lea","family":"Di Fina","role":"aut","roleDisplay":"VerfasserIn","display":"Di Fina, Lea"},{"role":"aut","display":"Mulkers, Maité","roleDisplay":"VerfasserIn","given":"Maité","family":"Mulkers"},{"roleDisplay":"VerfasserIn","display":"Laun, Lisa","role":"aut","family":"Laun","given":"Lisa"},{"family":"Li","given":"Lukas","display":"Li, Lukas","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Kupper","given":"Nadja","display":"Kupper, Nadja","roleDisplay":"VerfasserIn","role":"aut"},{"display":"Yue, Keyang","roleDisplay":"VerfasserIn","role":"aut","family":"Yue","given":"Keyang"},{"display":"Hoffknecht, Marie-Louise","roleDisplay":"VerfasserIn","role":"aut","family":"Hoffknecht","given":"Marie-Louise"},{"roleDisplay":"VerfasserIn","display":"Akhalkatsi, Anastassia","role":"aut","family":"Akhalkatsi","given":"Anastassia"},{"display":"Loew, Quentin","roleDisplay":"VerfasserIn","role":"aut","family":"Loew","given":"Quentin"},{"family":"Pircher","given":"Joachim","display":"Pircher, Joachim","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Escaig","given":"Raphael","display":"Escaig, Raphael","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Strasser","given":"Erwin","roleDisplay":"VerfasserIn","display":"Strasser, Erwin","role":"aut"},{"roleDisplay":"VerfasserIn","display":"Wichmann, Christian","role":"aut","family":"Wichmann","given":"Christian"},{"roleDisplay":"VerfasserIn","display":"Pekayvaz, Kami","role":"aut","family":"Pekayvaz","given":"Kami"},{"family":"Nieswandt","given":"Bernhard","display":"Nieswandt, Bernhard","roleDisplay":"VerfasserIn","role":"aut"},{"display":"Schulz, Christian","roleDisplay":"VerfasserIn","role":"aut","family":"Schulz","given":"Christian"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Robles, Maria S.","given":"Maria S.","family":"Robles"},{"roleDisplay":"VerfasserIn","display":"Kaiser, Rainer","role":"aut","family":"Kaiser","given":"Rainer"},{"given":"Steffen","family":"Massberg","role":"aut","roleDisplay":"VerfasserIn","display":"Massberg, Steffen"},{"given":"Robert","family":"Campbell","role":"aut","display":"Campbell, Robert","roleDisplay":"VerfasserIn"},{"family":"Nicolai","given":"Leo","display":"Nicolai, Leo","roleDisplay":"VerfasserIn","role":"aut"}],"title":[{"title_sort":"Aging platelets shift their hemostatic properties to inflammatory functions","subtitle":"platelets and thrombopoiesis","title":"Aging platelets shift their hemostatic properties to inflammatory functions"}]} | ||
| SRT | |a ANJUMAFRAMAGINGPLATE3202 | ||