Establishing an auxin-inducible GFP nanobody-based acute protein knockdown system to mimic hypomorphic mutations during early medaka embryogenesis
Creating hypomorphic mutations are crucial to study gene function in vivo, especially when null mutations result in (embryonic) lethality. This is especially the case for enzymes involved in glycosylation that, when mutated in human patients, are causing the disease congenital disorders of glycosyla...
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| Hauptverfasser: | , , , , , |
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| Dokumenttyp: | Article (Journal) Kapitel/Artikel |
| Sprache: | Englisch |
| Veröffentlicht: |
May 23, 2025
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| In: |
bioRxiv beta
Year: 2025, Pages: 1-22 |
| DOI: | 10.1101/2025.05.23.655727 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1101/2025.05.23.655727 Verlag, kostenfrei, Volltext: https://www.biorxiv.org/content/10.1101/2025.05.23.655727v1 |
| Verfasserangaben: | Kaisa Pakari, Sevinç Jakab, Encarnación Sánchez Salvador, Christian Thiel, Joachim Wittbrodt, Thomas Thumberger |
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