Expression of myosin heavy and light chains and phosphorylation of the phosphorylatable myosin light chain in the heart ventricle of the European hamster during hibernation and in summer
We investigated the expression of myosin subunits (myosin heavy chains) as well as light chains and thein vivo phosphorylation of the phosphorylatable myosin light chain in the heart ventricle of the adult male European hamster (Cricetus cricetus L.). Two myosin heavy chain isoenzymes could be detec...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
February 1992
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| In: |
Journal of muscle research and cell motility
Year: 1992, Volume: 13, Issue: 1, Pages: 64-70 |
| ISSN: | 1573-2657 |
| DOI: | 10.1007/BF01738429 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/BF01738429
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| Author Notes: | I. Morano, K. Adler, B. Agostini, and W. Hasselbach |
| Summary: | We investigated the expression of myosin subunits (myosin heavy chains) as well as light chains and thein vivo phosphorylation of the phosphorylatable myosin light chain in the heart ventricle of the adult male European hamster (Cricetus cricetus L.). Two myosin heavy chain isoenzymes could be detected under native and denaturing electrophoretic conditions having high (α-myosin heavy chain) and low (β-myosin heavy chain) enzymatic activity. Enzymatic activity ofα- andβ-myosin heavy chain revealed a different temperature dependency. When temperature increased ATPase activity of theα-myosin heavy chain isoenzyme increased relatively more than ATPase activity of theβ-myosin heavy chain isoenzyme. Summer animals expressed predominantly theβ-myosin heavy chain (79% of total myosin) while during hibernation theα-myosin heavy chain expression increased to 53% of total myosin. Winter-active hamsters kept at 22°C and 12 h day/night rhythm showed the same myosin heavy chain isoenzyme pattern as summer-active animals. Two myosin light chain forms were expressed in the ventricle of all animal groups. thein vivo phosphorylation level of the phosphorylatable myosin light chain decreased from 45% in summer-active hamster to 23% during hibernation. |
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| Item Description: | Gesehen am 21.07.2025 |
| Physical Description: | Online Resource |
| ISSN: | 1573-2657 |
| DOI: | 10.1007/BF01738429 |