l-Fucose residues on cellulose-based dialysis membranes: Quantification of membrane-associatedl-fucose and analysis of specific lectin binding
Contact of mononuclear human leukocytes with cellulose dialysis membranes may result in complement-independent cell activation, i.e. enhanced synthesis of cytokines, prostaglandins and an increase in β2-microglobulin synthesis. Cellular contact activation is specifically inhibited by the monosacchar...
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| Hauptverfasser: | , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
October 1995
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| In: |
Glycoconjugate journal
Year: 1995, Jahrgang: 12, Heft: 5, Pages: 632-638 |
| ISSN: | 1573-4986 |
| DOI: | 10.1007/BF00731258 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/BF00731258
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| Verfasserangaben: | Carsten Meißner, Reinhold Deppisch, Friederike Hug, Matthias Schulze, Eberhard Ritz, Horst Ludwig, Gertrud M. Hänsch |
| Zusammenfassung: | Contact of mononuclear human leukocytes with cellulose dialysis membranes may result in complement-independent cell activation, i.e. enhanced synthesis of cytokines, prostaglandins and an increase in β2-microglobulin synthesis. Cellular contact activation is specifically inhibited by the monosaccharidel-fucose suggesting that dialysis membrane associatedl-fucose residues are involved in leukocyte activation. In this study we have detected and quantitatedl-fucose on commercially-available cellulose dialysis membranes using two approaches. A sensitive enzymatic fluorescence assay detectedl-fucose after acid hydrolysis of flat sheet membranes. Values ranged from 79.3±3.6 to 90.2±5.0 pmol cm−2 for Hemophan® or Cuprophan® respectively. Enzymatic cleavage of terminal α-l-fucopyranoses with α-l-fucosidase yielded 7.7±3.3 pmoll-fucose per cm2 for Cuprophan. Enzymatic hydrolysis of the synthetic polymer membranes AN-69 and PC-PE did not yield detectable amounts ofl-fucose. In a second approach, binding of the fucose specific lectins ofLotus tetragonolobus andUlex europaeus (UEAI) demonstrated the presence of biologically accessiblel-fucose on the surface of cellulose membranes. Specific binding was observed with Cuprophan®, and up to 2.6±0.3 pmoll-fucose per cm2 was calculated to be present from Langmuir-type adsorption isotherms. The data presented are in line with the hypothesis that surface-associatedl-fucose residues on cellulose dialysis membranes participate in leukocyte contact activation. |
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| Beschreibung: | Gesehen am 29.07.2025 |
| Beschreibung: | Online Resource |
| ISSN: | 1573-4986 |
| DOI: | 10.1007/BF00731258 |