Single-cell and spatial genomic landscape of non-small cell lung cancer brain metastases
Brain metastases frequently develop in patients with non-small cell lung cancer (NSCLC) and are a common cause of cancer-related deaths, yet our understanding of the underlying human biology is limited. Here we performed multimodal single-nucleus RNA and T cell receptor, single-cell spatial and whol...
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| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
27 February 2025
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| In: |
Nature medicine
Year: 2025, Volume: 31, Issue: 4, Pages: 1351-1363 |
| ISSN: | 1546-170X |
| DOI: | 10.1038/s41591-025-03530-z |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41591-025-03530-z Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s41591-025-03530-z 
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| Author Notes: | Somnath Tagore, Lindsay Caprio, Amit Dipak Amin, Kresimir Bestak, Karan Luthria, Edridge D’Souza, Irving Barrera, Johannes C. Melms, Sharon Wu, Sinan Abuzaid, Yiping Wang, Viktoria Jakubikova, Peter Koch, D. Zack Brodtman, Banpreet Bawa, Sachin K. Deshmukh, Leon Ebel, Miguel A. Ibarra-Arellano, Abhinav Jaiswal, Carino Gurjao, Jana Biermann, Neha Shaikh, Priyanka Ramaradj, Yohanna Georgis, Galina G. Lagos, Matthew I. Ehrlich, Patricia Ho, Zachary H. Walsh, Meri Rogava, Michelle Garlin Politis, Devanik Biswas, Azzurra Cottarelli, Nikhil Rizvi, Catherine A. Shu, Benjamin Herzberg, Niroshana Anandasabapathy, George Sledge, Emmanuel Zorn, Peter Canoll, Jeffrey N. Bruce, Naiyer A. Rizvi, Alison M. Taylor, Anjali Saqi, Hanina Hibshoosh, Gary K. Schwartz, Brian S. Henick, Fei Chen, Denis Schapiro, Parin Shah & Benjamin Izar |
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| 245 | 1 | 0 | |a Single-cell and spatial genomic landscape of non-small cell lung cancer brain metastases |c Somnath Tagore, Lindsay Caprio, Amit Dipak Amin, Kresimir Bestak, Karan Luthria, Edridge D’Souza, Irving Barrera, Johannes C. Melms, Sharon Wu, Sinan Abuzaid, Yiping Wang, Viktoria Jakubikova, Peter Koch, D. Zack Brodtman, Banpreet Bawa, Sachin K. Deshmukh, Leon Ebel, Miguel A. Ibarra-Arellano, Abhinav Jaiswal, Carino Gurjao, Jana Biermann, Neha Shaikh, Priyanka Ramaradj, Yohanna Georgis, Galina G. Lagos, Matthew I. Ehrlich, Patricia Ho, Zachary H. Walsh, Meri Rogava, Michelle Garlin Politis, Devanik Biswas, Azzurra Cottarelli, Nikhil Rizvi, Catherine A. Shu, Benjamin Herzberg, Niroshana Anandasabapathy, George Sledge, Emmanuel Zorn, Peter Canoll, Jeffrey N. Bruce, Naiyer A. Rizvi, Alison M. Taylor, Anjali Saqi, Hanina Hibshoosh, Gary K. Schwartz, Brian S. Henick, Fei Chen, Denis Schapiro, Parin Shah & Benjamin Izar |
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| 520 | |a Brain metastases frequently develop in patients with non-small cell lung cancer (NSCLC) and are a common cause of cancer-related deaths, yet our understanding of the underlying human biology is limited. Here we performed multimodal single-nucleus RNA and T cell receptor, single-cell spatial and whole-genome sequencing of brain metastases and primary tumors of patients with treatment-naive NSCLC. Chromosomal instability (CIN) is a distinguishing genomic feature of brain metastases compared with primary tumors, which we validated through integrated analysis of molecular profiling and clinical data in 4,869 independent patients, and a new cohort of 12,275 patients with NSCLC. Unbiased analyses revealed transcriptional neural-like programs that strongly enriched in cancer cells from brain metastases, including a recurring, CINhigh cell subpopulation that preexists in primary tumors but strongly enriched in brain metastases, which was also recovered in matched single-cell spatial transcriptomics. Using multiplexed immunofluorescence in an independent cohort of treatment-naive pairs of primary tumors and brain metastases from the same patients with NSCLC, we validated genomic and tumor-microenvironmental findings and identified a cancer cell population characterized by neural features strongly enriched in brain metastases. This comprehensive analysis provides insights into human NSCLC brain metastasis biology and serves as an important resource for additional discovery. | ||
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