Rotavirus spreads in a spatially controlled manner
Rotavirus is an enteric virus that leads to 200,000 deaths worldwide every year. The live-cell imaging evaluating rotavirus infection of MA104 cells revealed that rotavirus replication and spread occurs in a spatially controlled manner. Specifically, following initial rotavirus infection, the infect...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
February-2 2025
|
| In: |
Cells
Year: 2025, Jahrgang: 14, Heft: 4, Pages: 1-17 |
| ISSN: | 2073-4409 |
| DOI: | 10.3390/cells14040313 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.3390/cells14040313 Verlag, kostenfrei, Volltext: https://www.mdpi.com/2073-4409/14/4/313 
|
| Verfasserangaben: | Gianna V. Passarelli, Patricio Doldan, Camila Metz-Zumaran, Yagmur Keser, Steeve Boulant and Megan L. Stanifer |
MARC
| LEADER | 00000naa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1932417028 | ||
| 003 | DE-627 | ||
| 005 | 20250804120532.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 250804s2025 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/cells14040313 |2 doi | |
| 035 | |a (DE-627)1932417028 | ||
| 035 | |a (DE-599)KXP1932417028 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Passarelli, Gianna V. |e VerfasserIn |0 (DE-588)1373119012 |0 (DE-627)1932419780 |4 aut | |
| 245 | 1 | 0 | |a Rotavirus spreads in a spatially controlled manner |c Gianna V. Passarelli, Patricio Doldan, Camila Metz-Zumaran, Yagmur Keser, Steeve Boulant and Megan L. Stanifer |
| 264 | 1 | |c February-2 2025 | |
| 300 | |b Illustrationen | ||
| 300 | |a 17 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Online veröffentlicht am 9. Februar 2025 | ||
| 500 | |a Gesehen am 04.08.2025 | ||
| 520 | |a Rotavirus is an enteric virus that leads to 200,000 deaths worldwide every year. The live-cell imaging evaluating rotavirus infection of MA104 cells revealed that rotavirus replication and spread occurs in a spatially controlled manner. Specifically, following initial rotavirus infection, the infected cells die, and the second round of infection occurs in the restricted area surrounding the initially infected cell. Interestingly, we found that the time required to establish the secondary infection is shorter compared to the time required for the initial infection. To determine if this increase in the kinetic of secondary infection was due to the early release of viruses or priming of the cells that are infected during the secondary infection, we used a combination of live-cell microscopy, trypsin neutralization assays, and the pharmacological inhibition of calcium signaling. Together, our results show that the second round of infection required rotavirus to be released and accessible to extracellular proteases. In addition, we found that the calcium wave induced upon rotavirus infection was critical for initial infection but did not play a role in the establishment of a secondary infection. Finally, we uncovered that high viral titers released from the initial infection were sufficient to accelerate the rate of the secondary infection. | ||
| 650 | 4 | |a rotavirus | |
| 650 | 4 | |a spatial virus replication | |
| 650 | 4 | |a virus spread | |
| 700 | 1 | |a Doldan, Patricio |d 1990- |e VerfasserIn |0 (DE-588)1258412055 |0 (DE-627)1804396702 |4 aut | |
| 700 | 1 | |a Metz Zumarán, Camila |d 1995- |e VerfasserIn |0 (DE-588)1217608419 |0 (DE-627)173178158X |4 aut | |
| 700 | 1 | |a Keser, Yagmur |e VerfasserIn |4 aut | |
| 700 | 1 | |a Boulant, Steeve |e VerfasserIn |0 (DE-588)1130687430 |0 (DE-627)884888509 |0 (DE-576)48692243X |4 aut | |
| 700 | 1 | |a Stanifer, Megan |e VerfasserIn |0 (DE-588)1130687643 |0 (DE-627)884888835 |0 (DE-576)486922367 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cells |d Basel : MDPI, 2012 |g 14(2025), 4 vom: Feb., Artikel-ID 313, Seite 1-17 |h Online-Ressource |w (DE-627)718622081 |w (DE-600)2661518-6 |w (DE-576)365412112 |x 2073-4409 |7 nnas |a Rotavirus spreads in a spatially controlled manner |
| 773 | 1 | 8 | |g volume:14 |g year:2025 |g number:4 |g month:02 |g elocationid:313 |g pages:1-17 |g extent:17 |a Rotavirus spreads in a spatially controlled manner |
| 856 | 4 | 0 | |u https://doi.org/10.3390/cells14040313 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.mdpi.com/2073-4409/14/4/313 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20250804 | ||
| 993 | |a Article | ||
| 994 | |a 2025 | ||
| 998 | |g 1130687430 |a Boulant, Steeve |m 1130687430:Boulant, Steeve |d 50000 |e 50000PB1130687430 |k 0/50000/ |p 5 | ||
| 999 | |a KXP-PPN1932417028 |e 4752941406 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"pubHistory":["1.2012 -"],"title":[{"title":"Cells","subtitle":"open access journal","title_sort":"Cells"}],"language":["eng"],"part":{"issue":"4","extent":"17","pages":"1-17","year":"2025","text":"14(2025), 4 vom: Feb., Artikel-ID 313, Seite 1-17","volume":"14"},"id":{"eki":["718622081"],"zdb":["2661518-6"],"issn":["2073-4409"]},"physDesc":[{"extent":"Online-Ressource"}],"disp":"Rotavirus spreads in a spatially controlled mannerCells","note":["Gesehen am 09.05.12"],"type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"718622081","origin":[{"publisherPlace":"Basel","dateIssuedKey":"2012","dateIssuedDisp":"2012-","publisher":"MDPI"}]}],"note":["Online veröffentlicht am 9. Februar 2025","Gesehen am 04.08.2025"],"physDesc":[{"noteIll":"Illustrationen","extent":"17 S."}],"id":{"eki":["1932417028"],"doi":["10.3390/cells14040313"]},"type":{"media":"Online-Ressource","bibl":"article-journal"},"recId":"1932417028","origin":[{"dateIssuedDisp":"February-2 2025","dateIssuedKey":"2025"}],"person":[{"family":"Passarelli","display":"Passarelli, Gianna V.","role":"aut","given":"Gianna V."},{"given":"Patricio","role":"aut","display":"Doldan, Patricio","family":"Doldan"},{"family":"Metz Zumarán","display":"Metz Zumarán, Camila","role":"aut","given":"Camila"},{"display":"Keser, Yagmur","role":"aut","given":"Yagmur","family":"Keser"},{"family":"Boulant","display":"Boulant, Steeve","given":"Steeve","role":"aut"},{"role":"aut","given":"Megan","display":"Stanifer, Megan","family":"Stanifer"}],"name":{"displayForm":["Gianna V. Passarelli, Patricio Doldan, Camila Metz-Zumaran, Yagmur Keser, Steeve Boulant and Megan L. Stanifer"]},"language":["eng"],"title":[{"title_sort":"Rotavirus spreads in a spatially controlled manner","title":"Rotavirus spreads in a spatially controlled manner"}]} | ||
| SRT | |a PASSARELLIROTAVIRUSS2202 | ||