Extrahepatic biliary obstruction impairs microvascular perfusion and increases leukocyte adhesion in rat liver
To determine if disturbances of the liver microcirculation may be of pathophysiological relevance for liver damage during acute biliary obstruction, we studied the effects of bile duct ligation (BDL) on hepatic microhemodynamics and leukocyte adhesion in rat liver in vivo. Male Wistar rats were subj...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
November 1997
|
| In: |
Hepatology
Year: 1997, Volume: 26, Issue: 5, Pages: 1085-1091 |
| ISSN: | 1527-3350 |
| DOI: | 10.1002/hep.510260501 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/hep.510260501 Verlag, lizenzpflichtig, Volltext: https://journals.lww.com/hep/abstract/1997/11000/extrahepatic_biliary_obstruction_impairs.2.aspx 
|
| Author Notes: | T.A. Koeppel, M. Trauner, J.C. Baas, J.C. Thies, S.F. Schlosser, S. Post, M. Gebhard, C. Herfarth, J.L. Boyer, G. Otto |
MARC
| LEADER | 00000naa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1934742104 | ||
| 003 | DE-627 | ||
| 005 | 20250826143146.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 250826s1997 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/hep.510260501 |2 doi | |
| 035 | |a (DE-627)1934742104 | ||
| 035 | |a (DE-599)KXP1934742104 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Koeppel, Thomas |e VerfasserIn |0 (DE-588)1076689701 |0 (DE-627)835259889 |0 (DE-576)189152613 |4 aut | |
| 245 | 1 | 0 | |a Extrahepatic biliary obstruction impairs microvascular perfusion and increases leukocyte adhesion in rat liver |c T.A. Koeppel, M. Trauner, J.C. Baas, J.C. Thies, S.F. Schlosser, S. Post, M. Gebhard, C. Herfarth, J.L. Boyer, G. Otto |
| 264 | 1 | |c November 1997 | |
| 300 | |a 7 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 26.08.2025 | ||
| 520 | |a To determine if disturbances of the liver microcirculation may be of pathophysiological relevance for liver damage during acute biliary obstruction, we studied the effects of bile duct ligation (BDL) on hepatic microhemodynamics and leukocyte adhesion in rat liver in vivo. Male Wistar rats were subjected to BDL for 3 days and 7 days, respectively. Sham-operated controls underwent laparotomy without BDL. After 3 days, intravital fluorescence microscopy (IVM) and hydrogen gas (H2) clearance were performed to study hepatic microvascular perfusion. Furthermore, leukocyte-endothelial cell interactions were assessed by IVM. Intercellular adhesion molecule 1 (ICAM-1) protein expression was studied by Western blot analysis and tissue immunofluorescence after 3 and 7 days, respectively. Analysis of microvascular perfusion by IVM revealed a marked impairment of sinusoidal perfusion after 3 days. Assessment of H2 clearance confirmed that overall hepatic microvascular perfusion was decreased. In addition, increased leukocyte adhesion in sinusoids and venules could be observed. A concomitant increase of ICAM-1 expression in liver tissue was also noted within the first week after BDL. Our results show that BDL is followed by a marked depression of the hepatic microcirculation and increased leukocyte adhesion in vivo within 3 to 7 days. Together, these findings suggest that deficits in microvascular perfusion and increased neutrophil infiltration may represent a potential source of liver injury during acute biliary obstruction. | ||
| 700 | 1 | |a Trauner, Michael |d 1964- |e VerfasserIn |0 (DE-588)1141405369 |0 (DE-627)1000309215 |0 (DE-576)494386401 |4 aut | |
| 700 | 1 | |a Baas, Jens |d 1967- |e VerfasserIn |0 (DE-588)112237562X |0 (DE-627)875662420 |0 (DE-576)175932921 |4 aut | |
| 700 | 1 | |a Thies, Jochen |e VerfasserIn |0 (DE-588)1082449903 |0 (DE-627)847509532 |0 (DE-576)455438854 |4 aut | |
| 700 | 1 | |a Schlosser, S. F. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Post, Stefan |d 1954- |e VerfasserIn |0 (DE-588)1026227518 |0 (DE-627)726294667 |0 (DE-576)371524598 |4 aut | |
| 700 | 1 | |a Gebhard, Martha-Maria |d 1948- |e VerfasserIn |0 (DE-588)1076763278 |0 (DE-627)835312879 |0 (DE-576)445612789 |4 aut | |
| 700 | 1 | |a Herfarth, Christian |d 1933-2014 |e VerfasserIn |0 (DE-588)120706385 |0 (DE-627)080841562 |0 (DE-576)213333414 |4 aut | |
| 700 | 1 | |a Boyer, James L. |d 1936- |e VerfasserIn |0 (DE-588)130954187 |0 (DE-627)707598575 |0 (DE-576)298406993 |4 aut | |
| 700 | 1 | |a Otto, Gerd |d 1944- |e VerfasserIn |0 (DE-588)1235891895 |0 (DE-627)1761126156 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Hepatology |d [Alphen aan den Rijn] : Wolters Kluwer Health, 1981 |g 26(1997), 5, Seite 1085-1091 |h Online-Ressource |w (DE-627)268132003 |w (DE-600)1472120-X |w (DE-576)077450183 |x 1527-3350 |7 nnas |a Extrahepatic biliary obstruction impairs microvascular perfusion and increases leukocyte adhesion in rat liver |
| 773 | 1 | 8 | |g volume:26 |g year:1997 |g number:5 |g pages:1085-1091 |g extent:7 |a Extrahepatic biliary obstruction impairs microvascular perfusion and increases leukocyte adhesion in rat liver |
| 856 | 4 | 0 | |u https://doi.org/10.1002/hep.510260501 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://journals.lww.com/hep/abstract/1997/11000/extrahepatic_biliary_obstruction_impairs.2.aspx |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20250826 | ||
| 993 | |a Article | ||
| 994 | |a 1997 | ||
| 998 | |g 1235891895 |a Otto, Gerd |m 1235891895:Otto, Gerd |d 910000 |d 910200 |e 910000PO1235891895 |e 910200PO1235891895 |k 0/910000/ |k 1/910000/910200/ |p 10 |y j | ||
| 998 | |g 120706385 |a Herfarth, Christian |m 120706385:Herfarth, Christian |d 910000 |d 910200 |e 910000PH120706385 |e 910200PH120706385 |k 0/910000/ |k 1/910000/910200/ |p 8 | ||
| 998 | |g 1076763278 |a Gebhard, Martha-Maria |m 1076763278:Gebhard, Martha-Maria |d 910000 |d 910200 |e 910000PG1076763278 |e 910200PG1076763278 |k 0/910000/ |k 1/910000/910200/ |p 7 | ||
| 998 | |g 1026227518 |a Post, Stefan |m 1026227518:Post, Stefan |d 910000 |d 910200 |e 910000PP1026227518 |e 910200PP1026227518 |k 0/910000/ |k 1/910000/910200/ |p 6 | ||
| 998 | |g 1082449903 |a Thies, Jochen |m 1082449903:Thies, Jochen |d 910000 |d 910200 |e 910000PT1082449903 |e 910200PT1082449903 |k 0/910000/ |k 1/910000/910200/ |p 4 | ||
| 998 | |g 112237562X |a Baas, Jens |m 112237562X:Baas, Jens |d 910000 |d 910200 |e 910000PB112237562X |e 910200PB112237562X |k 0/910000/ |k 1/910000/910200/ |p 3 | ||
| 998 | |g 1076689701 |a Koeppel, Thomas |m 1076689701:Koeppel, Thomas |d 910000 |d 910200 |e 910000PK1076689701 |e 910200PK1076689701 |k 0/910000/ |k 1/910000/910200/ |p 1 |x j | ||
| 999 | |a KXP-PPN1934742104 |e 4764318407 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title":"Extrahepatic biliary obstruction impairs microvascular perfusion and increases leukocyte adhesion in rat liver","title_sort":"Extrahepatic biliary obstruction impairs microvascular perfusion and increases leukocyte adhesion in rat liver"}],"person":[{"role":"aut","display":"Koeppel, Thomas","roleDisplay":"VerfasserIn","given":"Thomas","family":"Koeppel"},{"family":"Trauner","given":"Michael","display":"Trauner, Michael","roleDisplay":"VerfasserIn","role":"aut"},{"given":"Jens","family":"Baas","role":"aut","display":"Baas, Jens","roleDisplay":"VerfasserIn"},{"given":"Jochen","family":"Thies","role":"aut","display":"Thies, Jochen","roleDisplay":"VerfasserIn"},{"given":"S. F.","family":"Schlosser","role":"aut","roleDisplay":"VerfasserIn","display":"Schlosser, S. F."},{"given":"Stefan","family":"Post","role":"aut","display":"Post, Stefan","roleDisplay":"VerfasserIn"},{"given":"Martha-Maria","family":"Gebhard","role":"aut","display":"Gebhard, Martha-Maria","roleDisplay":"VerfasserIn"},{"display":"Herfarth, Christian","roleDisplay":"VerfasserIn","role":"aut","family":"Herfarth","given":"Christian"},{"given":"James L.","family":"Boyer","role":"aut","display":"Boyer, James L.","roleDisplay":"VerfasserIn"},{"family":"Otto","given":"Gerd","roleDisplay":"VerfasserIn","display":"Otto, Gerd","role":"aut"}],"recId":"1934742104","language":["eng"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Gesehen am 26.08.2025"],"id":{"doi":["10.1002/hep.510260501"],"eki":["1934742104"]},"origin":[{"dateIssuedKey":"1997","dateIssuedDisp":"November 1997"}],"name":{"displayForm":["T.A. Koeppel, M. Trauner, J.C. Baas, J.C. Thies, S.F. Schlosser, S. Post, M. Gebhard, C. Herfarth, J.L. Boyer, G. Otto"]},"relHost":[{"id":{"issn":["1527-3350"],"zdb":["1472120-X"],"doi":["10.1002/(ISSN)1527-3350"],"eki":["268132003"]},"origin":[{"dateIssuedDisp":"1981-","publisher":"Wolters Kluwer Health ; Saunders ; Wiley Interscience","dateIssuedKey":"1981","publisherPlace":"[Alphen aan den Rijn] ; Philadelphia, Pa. ; New York [u.a.]"}],"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"Hepatology","subtitle":"official journal of the American Association for the Study of Liver Diseases","title":"Hepatology"}],"part":{"volume":"26","text":"26(1997), 5, Seite 1085-1091","extent":"7","year":"1997","pages":"1085-1091","issue":"5"},"pubHistory":["1.1981 -"],"language":["eng"],"recId":"268132003","type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 28.02.08"],"disp":"Extrahepatic biliary obstruction impairs microvascular perfusion and increases leukocyte adhesion in rat liverHepatology"}],"physDesc":[{"extent":"7 S."}]} | ||
| SRT | |a KOEPPELTHOEXTRAHEPAT1997 | ||