MicronuclAI enables automated quantification of micronuclei for assessment of chromosomal instability
Chromosomal instability (CIN) is a hallmark of cancer that drives metastasis, immune evasion and treatment resistance. CIN may result from chromosome mis-segregation errors and excessive chromatin is frequently packaged in micronuclei (MN), which can be enumerated to quantify CIN. The assessment of...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
04 March 2025
|
| In: |
Communications biology
Year: 2025, Jahrgang: 8, Heft: 1, Pages: 1-10 |
| ISSN: | 2399-3642 |
| DOI: | 10.1038/s42003-025-07796-4 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s42003-025-07796-4 Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s42003-025-07796-4 
|
| Verfasserangaben: | Miguel A. Ibarra-Arellano, Lindsay A. Caprio, Aroj Hada, Niklas Stotzem, Luke L. Cai, Shivem B. Shah, Zachary H. Walsh, Johannes C. Melms, Florian Wünneman, Kresimir Bestak, Ibrahim Mansaray, Benjamin Izar & Denis Schapiro |
MARC
| LEADER | 00000naa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1935234374 | ||
| 003 | DE-627 | ||
| 005 | 20250903114933.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 250903s2025 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s42003-025-07796-4 |2 doi | |
| 035 | |a (DE-627)1935234374 | ||
| 035 | |a (DE-599)KXP1935234374 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Ibarra-Arellano, Miguel A. |e VerfasserIn |0 (DE-588)1281451614 |0 (DE-627)183710607X |4 aut | |
| 245 | 1 | 0 | |a MicronuclAI enables automated quantification of micronuclei for assessment of chromosomal instability |c Miguel A. Ibarra-Arellano, Lindsay A. Caprio, Aroj Hada, Niklas Stotzem, Luke L. Cai, Shivem B. Shah, Zachary H. Walsh, Johannes C. Melms, Florian Wünneman, Kresimir Bestak, Ibrahim Mansaray, Benjamin Izar & Denis Schapiro |
| 264 | 1 | |c 04 March 2025 | |
| 300 | |b Illustrationen | ||
| 300 | |a 10 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 03.09.2025 | ||
| 520 | |a Chromosomal instability (CIN) is a hallmark of cancer that drives metastasis, immune evasion and treatment resistance. CIN may result from chromosome mis-segregation errors and excessive chromatin is frequently packaged in micronuclei (MN), which can be enumerated to quantify CIN. The assessment of CIN remains a predominantly manual and time-consuming task. Here, we present micronuclAI, a pipeline for automated and reliable quantification of MN of varying size and morphology in cells stained only for DNA. micronuclAI can achieve close to human-level performance on various human and murine cancer cell line datasets. The pipeline achieved a Pearson’s correlation of 0.9278 on images obtained at 10X magnification. We tested the approach in otherwise isogenic cell lines in which we genetically dialed up or down CIN rates, and on several publicly available image datasets where we achieved a Pearson’s correlation of 0.9620. Given the increasing interest in developing therapies for CIN-driven cancers, this method provides an important, scalable, and rapid approach to quantifying CIN on images that are routinely obtained for research purposes. We release a GUI-implementation for easy access and utilization of the pipeline. | ||
| 650 | 4 | |a Cancer | |
| 650 | 4 | |a Image processing | |
| 650 | 4 | |a Software | |
| 700 | 1 | |a Caprio, Lindsay A. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Hada, Aroj |e VerfasserIn |0 (DE-588)137540587X |0 (DE-627)1935235699 |4 aut | |
| 700 | 1 | |a Stotzem, Niklas |e VerfasserIn |4 aut | |
| 700 | 1 | |a Cai, Luke L. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Shah, Shivem B. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Walsh, Zachary H. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Melms, Johannes C. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wünneman, Florian |e VerfasserIn |4 aut | |
| 700 | 1 | |a Bestak, Kresimir |e VerfasserIn |0 (DE-588)1372834605 |0 (DE-627)1932182837 |4 aut | |
| 700 | 1 | |a Mansaray, Ibrahim |e VerfasserIn |0 (DE-588)1375406191 |0 (DE-627)1935236180 |4 aut | |
| 700 | 1 | |a Izar, Benjamin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schapiro, Denis |d 1986- |e VerfasserIn |0 (DE-588)1165616769 |0 (DE-627)1029684693 |0 (DE-576)51047666X |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Communications biology |d London : Springer Nature, 2018 |g 8(2025), 1, Artikel-ID 361, Seite 1-10 |h Online-Ressource |w (DE-627)101369080X |w (DE-600)2919698-X |w (DE-576)499739132 |x 2399-3642 |7 nnas |a MicronuclAI enables automated quantification of micronuclei for assessment of chromosomal instability |
| 773 | 1 | 8 | |g volume:8 |g year:2025 |g number:1 |g elocationid:361 |g pages:1-10 |g extent:10 |a MicronuclAI enables automated quantification of micronuclei for assessment of chromosomal instability |
| 856 | 4 | 0 | |u https://doi.org/10.1038/s42003-025-07796-4 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/s42003-025-07796-4 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20250903 | ||
| 993 | |a Article | ||
| 994 | |a 2025 | ||
| 998 | |g 1165616769 |a Schapiro, Denis |m 1165616769:Schapiro, Denis |d 910000 |d 912900 |e 910000PS1165616769 |e 912900PS1165616769 |k 0/910000/ |k 1/910000/912900/ |p 13 |y j | ||
| 998 | |g 1375406191 |a Mansaray, Ibrahim |m 1375406191:Mansaray, Ibrahim |d 140000 |e 140000PM1375406191 |k 0/140000/ |p 11 | ||
| 998 | |g 1372834605 |a Bestak, Kresimir |m 1372834605:Bestak, Kresimir |d 910000 |d 912900 |e 910000PB1372834605 |e 912900PB1372834605 |k 0/910000/ |k 1/910000/912900/ |p 10 | ||
| 998 | |g 137540587X |a Hada, Aroj |m 137540587X:Hada, Aroj |d 910000 |d 912900 |e 910000PH137540587X |e 912900PH137540587X |k 0/910000/ |k 1/910000/912900/ |p 3 | ||
| 998 | |g 1281451614 |a Ibarra Arellano, Miguel A. |m 1281451614:Ibarra Arellano, Miguel A. |d 910000 |d 912900 |e 910000PI1281451614 |e 912900PI1281451614 |k 0/910000/ |k 1/910000/912900/ |p 1 |x j | ||
| 999 | |a KXP-PPN1935234374 |e 4767221331 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1935234374","name":{"displayForm":["Miguel A. Ibarra-Arellano, Lindsay A. Caprio, Aroj Hada, Niklas Stotzem, Luke L. Cai, Shivem B. Shah, Zachary H. Walsh, Johannes C. Melms, Florian Wünneman, Kresimir Bestak, Ibrahim Mansaray, Benjamin Izar & Denis Schapiro"]},"physDesc":[{"noteIll":"Illustrationen","extent":"10 S."}],"relHost":[{"disp":"MicronuclAI enables automated quantification of micronuclei for assessment of chromosomal instabilityCommunications biology","part":{"year":"2025","text":"8(2025), 1, Artikel-ID 361, Seite 1-10","pages":"1-10","extent":"10","volume":"8","issue":"1"},"type":{"media":"Online-Ressource","bibl":"periodical"},"origin":[{"publisherPlace":"London","dateIssuedDisp":"[2018]-","publisher":"Springer Nature"}],"title":[{"title_sort":"Communications biology","title":"Communications biology"}],"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"recId":"101369080X","pubHistory":["1 (2018)-"],"id":{"zdb":["2919698-X"],"issn":["2399-3642"],"eki":["101369080X"]}}],"id":{"eki":["1935234374"],"doi":["10.1038/s42003-025-07796-4"]},"person":[{"family":"Ibarra-Arellano","display":"Ibarra-Arellano, Miguel A.","given":"Miguel A.","role":"aut"},{"family":"Caprio","display":"Caprio, Lindsay A.","given":"Lindsay A.","role":"aut"},{"given":"Aroj","role":"aut","display":"Hada, Aroj","family":"Hada"},{"role":"aut","given":"Niklas","display":"Stotzem, Niklas","family":"Stotzem"},{"role":"aut","given":"Luke L.","display":"Cai, Luke L.","family":"Cai"},{"role":"aut","given":"Shivem B.","family":"Shah","display":"Shah, Shivem B."},{"display":"Walsh, Zachary H.","family":"Walsh","role":"aut","given":"Zachary H."},{"given":"Johannes C.","role":"aut","family":"Melms","display":"Melms, Johannes C."},{"display":"Wünneman, Florian","family":"Wünneman","given":"Florian","role":"aut"},{"family":"Bestak","display":"Bestak, Kresimir","given":"Kresimir","role":"aut"},{"role":"aut","given":"Ibrahim","display":"Mansaray, Ibrahim","family":"Mansaray"},{"given":"Benjamin","role":"aut","display":"Izar, Benjamin","family":"Izar"},{"family":"Schapiro","display":"Schapiro, Denis","role":"aut","given":"Denis"}],"language":["eng"],"note":["Gesehen am 03.09.2025"],"title":[{"title_sort":"MicronuclAI enables automated quantification of micronuclei for assessment of chromosomal instability","title":"MicronuclAI enables automated quantification of micronuclei for assessment of chromosomal instability"}],"origin":[{"dateIssuedKey":"2025","dateIssuedDisp":"04 March 2025"}],"type":{"media":"Online-Ressource","bibl":"article-journal"}} | ||
| SRT | |a IBARRAARELMICRONUCLA0420 | ||