Deep intravital brain tumor imaging enabled by tailored three-photon microscopy and analysis
Intravital 2P-microscopy enables the longitudinal study of brain tumor biology in superficial mouse cortex layers. Intravital microscopy of the white matter, an important route of glioblastoma invasion and recurrence, has not been feasible, due to low signal-to-noise ratios and insufficient spatiote...
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
10 September 2024
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| In: |
Nature Communications
Year: 2024, Jahrgang: 15, Pages: 1-21 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-024-51432-4 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41467-024-51432-4 Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41467-024-51432-4 
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| Verfasserangaben: | Marc Cicero Schubert, Stella Judith Soyka, Amr Tamimi, Emanuel Maus, Julian Schroers, Niklas Wißmann, Ekin Reyhan, Svenja Kristin Tetzlaff, Yvonne Yang, Robert Denninger, Robin Peretzke, Carlo Beretta, Michael Drumm, Alina Heuer, Verena Buchert, Alicia Steffens, Jordain Walshon, Kathleen McCortney, Sabine Heiland, Martin Bendszus, Peter Neher, Anna Golebiewska, Wolfgang Wick, Frank Winkler, Michael O. Breckwoldt, Anna Kreshuk, Thomas Kuner, Craig Horbinski, Felix Tobias Kurz, Robert Prevedel, and Varun Venkataramani |
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| 245 | 1 | 0 | |a Deep intravital brain tumor imaging enabled by tailored three-photon microscopy and analysis |c Marc Cicero Schubert, Stella Judith Soyka, Amr Tamimi, Emanuel Maus, Julian Schroers, Niklas Wißmann, Ekin Reyhan, Svenja Kristin Tetzlaff, Yvonne Yang, Robert Denninger, Robin Peretzke, Carlo Beretta, Michael Drumm, Alina Heuer, Verena Buchert, Alicia Steffens, Jordain Walshon, Kathleen McCortney, Sabine Heiland, Martin Bendszus, Peter Neher, Anna Golebiewska, Wolfgang Wick, Frank Winkler, Michael O. Breckwoldt, Anna Kreshuk, Thomas Kuner, Craig Horbinski, Felix Tobias Kurz, Robert Prevedel, and Varun Venkataramani |
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| 520 | |a Intravital 2P-microscopy enables the longitudinal study of brain tumor biology in superficial mouse cortex layers. Intravital microscopy of the white matter, an important route of glioblastoma invasion and recurrence, has not been feasible, due to low signal-to-noise ratios and insufficient spatiotemporal resolution. Here, we present an intravital microscopy and artificial intelligence-based analysis workflow (Deep3P) that enables longitudinal deep imaging of glioblastoma up to a depth of 1.2 mm. We find that perivascular invasion is the preferred invasion route into the corpus callosum and uncover two vascular mechanisms of glioblastoma migration in the white matter. Furthermore, we observe morphological changes after white matter infiltration, a potential basis of an imaging biomarker during early glioblastoma colonization. Taken together, Deep3P allows for a non-invasive intravital investigation of brain tumor biology and its tumor microenvironment at subcortical depths explored, opening up opportunities for studying the neuroscience of brain tumors and other model systems. | ||
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