Efflux pumps and membrane permeability contribute to intrinsic antibiotic resistance in Mycobacterium abscessus
Mycobacterium abscessus is a pulmonary pathogen that exhibits intrinsic resistance to antibiotics, but the factors driving this resistance are incompletely understood. Insufficient intracellular drug accumulation could explain broad-spectrum resistance, but whether antibiotics fail to accumulate in...
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| Hauptverfasser: | , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
April 10, 2025
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| In: |
PLoS pathogens
Year: 2025, Jahrgang: 21, Heft: 4, Pages: 1-28 |
| ISSN: | 1553-7374 |
| DOI: | 10.1371/journal.ppat.1013027 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1371/journal.ppat.1013027 Verlag, kostenfrei, Volltext: https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1013027 
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| Verfasserangaben: | Kerry McGowen, Tobias Funck, Xin Wang, Samuel Zinga, Ian D. Wolf, Chidiebere Akusobi, Claudia M. Denkinger, Eric J. Rubin, Mark R. Sullivan |
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| 245 | 1 | 0 | |a Efflux pumps and membrane permeability contribute to intrinsic antibiotic resistance in Mycobacterium abscessus |c Kerry McGowen, Tobias Funck, Xin Wang, Samuel Zinga, Ian D. Wolf, Chidiebere Akusobi, Claudia M. Denkinger, Eric J. Rubin, Mark R. Sullivan |
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| 520 | |a Mycobacterium abscessus is a pulmonary pathogen that exhibits intrinsic resistance to antibiotics, but the factors driving this resistance are incompletely understood. Insufficient intracellular drug accumulation could explain broad-spectrum resistance, but whether antibiotics fail to accumulate in M. abscessus and the mechanisms required for drug exclusion remain poorly understood. We measured antibiotic accumulation in M. abscessus using mass spectrometry and found a wide range of drug accumulation across clinically relevant antibiotics. Of these compounds, linezolid accumulates the least, suggesting that inadequate uptake impacts its efficacy. We utilized transposon mutagenesis screening to identify genes that cause linezolid resistance and found multiple transporters that promote membrane permeability or efflux, including an uncharacterized protein that effluxes linezolid and several chemically related antibiotics. This demonstrates that membrane permeability and drug efflux are critical mechanisms of antibiotic resistance in M. abscessus and suggests that targeting membrane transporters could potentiate the efficacy of certain antibiotics. | ||
| 650 | 4 | |a Antibiotic resistance | |
| 650 | 4 | |a Antibiotics | |
| 650 | 4 | |a Cell walls | |
| 650 | 4 | |a Liquid chromatography-mass spectrometry | |
| 650 | 4 | |a Membrane proteins | |
| 650 | 4 | |a Mycobacteria | |
| 650 | 4 | |a Mycobacterium tuberculosis | |
| 650 | 4 | |a Permeability | |
| 700 | 1 | |a Funck, Tobias |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wang, Xin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Zinga, Samuel |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wolf, Ian D. |e VerfasserIn |4 aut | |
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| 700 | 1 | |a Rubin, Eric J. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Sullivan, Mark R. |e VerfasserIn |4 aut | |
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