Detection of SYT±SSX1/2 fusion transcripts by reverse transcriptase-polymerase chain reaction (RT-PCR) is a valuable diagnostic tool in synovial sarcoma
Cytogenetically, most synovial sarcomas are characterised by a specific chromosomal translocation [(X;18)(p11.2;q11.2)], which results in the generation of fusion transcripts comprising SYT (18q11) and either SSX1 or SSX2 (Xp11) sequences. By using a sensitive reverse transcriptase-polymerase chain...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
1998
|
| In: |
European journal of cancer
Year: 1998, Volume: 34, Issue: 13, Pages: 2087-2093 |
| ISSN: | 1879-0852 |
| DOI: | 10.1016/S0959-8049(98)00240-8 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/S0959-8049(98)00240-8 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0959804998002408 
|
| Author Notes: | F. Willeke, G. Mechtersheimer, M. Schwarzbach, J. Weitz, D. Zimmer, T. Lehnert, Ch. Herfarth, M. von Knebel Doeberitz, R. Ridder |
MARC
| LEADER | 00000naa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 193747352X | ||
| 003 | DE-627 | ||
| 005 | 20251001103910.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 251001s1998 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/S0959-8049(98)00240-8 |2 doi | |
| 035 | |a (DE-627)193747352X | ||
| 035 | |a (DE-599)KXP193747352X | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Willeke, Frank |d 1963- |e VerfasserIn |0 (DE-588)1084359758 |0 (DE-627)848798902 |0 (DE-576)170761568 |4 aut | |
| 245 | 1 | 0 | |a Detection of SYT±SSX1/2 fusion transcripts by reverse transcriptase-polymerase chain reaction (RT-PCR) is a valuable diagnostic tool in synovial sarcoma |c F. Willeke, G. Mechtersheimer, M. Schwarzbach, J. Weitz, D. Zimmer, T. Lehnert, Ch. Herfarth, M. von Knebel Doeberitz, R. Ridder |
| 264 | 1 | |c 1998 | |
| 300 | |a 7 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Online verfügbar 3. Dezember 1998 | ||
| 500 | |a Gesehen am 01.10.2025 | ||
| 520 | |a Cytogenetically, most synovial sarcomas are characterised by a specific chromosomal translocation [(X;18)(p11.2;q11.2)], which results in the generation of fusion transcripts comprising SYT (18q11) and either SSX1 or SSX2 (Xp11) sequences. By using a sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) protocol, specific SYT-SSX1/2 fusion transcripts were detected in 10 histopathologically confirmed synovial sarcomas. Control tumours with morphological spindle cell patterns mimicking monophasic synovial sarcoma tested negative (18/19) in the RT-PCR protocol, with the exception of one spindle cell sarcoma originally classified as a fibrosarcoma. Furthermore, the established RT-PCR protocol was used to evaluate the feasibility of SYT-SSX1/2 fusion transcript detection for minimal residual disease analysis. Analyses of surgical margins revealed a fusion transcript in two of four operations for synovial sarcoma analysed, one of which was diagnosed with tumour free margins by conventional histopathology. These data suggest that the RT-PCR amplification of SYT-SSX1/2 fusion transcripts is a valuable tool in the differentiation of synovial sarcomas, especially in cases of equivocal morphology. Additionally, the RT-PCR approach may be used for the detection of residual tumour cells in synovial sarcoma patients. | ||
| 650 | 4 | |a minimal residual disease | |
| 650 | 4 | |a RT-PCR | |
| 650 | 4 | |a sarcoma | |
| 650 | 4 | |a translocation | |
| 700 | 1 | |a Mechtersheimer, Gunhild |e VerfasserIn |0 (DE-588)1021953024 |0 (DE-627)715842595 |0 (DE-576)170441598 |4 aut | |
| 700 | 1 | |a Schwarzbach, Matthias |d 1966- |e VerfasserIn |0 (DE-588)1077763433 |0 (DE-627)837470064 |0 (DE-576)184627036 |4 aut | |
| 700 | 1 | |a Weitz, Jürgen |e VerfasserIn |0 (DE-588)1022345974 |0 (DE-627)716954451 |0 (DE-576)171632176 |4 aut | |
| 700 | 1 | |a Zimmer, D. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Lehnert, Thomas |e VerfasserIn |0 (DE-588)1076334229 |0 (DE-627)834714566 |0 (DE-576)445208724 |4 aut | |
| 700 | 1 | |a Herfarth, Christian |d 1933-2014 |e VerfasserIn |0 (DE-588)120706385 |0 (DE-627)080841562 |0 (DE-576)213333414 |4 aut | |
| 700 | 1 | |a Knebel Doeberitz, Magnus von |e VerfasserIn |0 (DE-588)1022165291 |0 (DE-627)71693289X |0 (DE-576)364934239 |4 aut | |
| 700 | 1 | |a Ridder, Rüdiger |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t European journal of cancer |d Amsterdam [u.a.] : Elsevier, 1992 |g 34(1998), 13, Seite 2087-2093 |w (DE-627)266883400 |w (DE-600)1468190-0 |w (DE-576)090954173 |x 1879-0852 |7 nnas |a Detection of SYT±SSX1/2 fusion transcripts by reverse transcriptase-polymerase chain reaction (RT-PCR) is a valuable diagnostic tool in synovial sarcoma |
| 773 | 1 | 8 | |g volume:34 |g year:1998 |g number:13 |g pages:2087-2093 |g extent:7 |a Detection of SYT±SSX1/2 fusion transcripts by reverse transcriptase-polymerase chain reaction (RT-PCR) is a valuable diagnostic tool in synovial sarcoma |
| 856 | 4 | 0 | |u https://doi.org/10.1016/S0959-8049(98)00240-8 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S0959804998002408 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20251001 | ||
| 994 | |a 1998 | ||
| 998 | |g 1022165291 |a Knebel Doeberitz, Magnus von |m 1022165291:Knebel Doeberitz, Magnus von |d 910000 |d 912000 |e 910000PK1022165291 |e 912000PK1022165291 |k 0/910000/ |k 1/910000/912000/ |p 8 | ||
| 998 | |g 120706385 |a Herfarth, Christian |m 120706385:Herfarth, Christian |d 910000 |d 910200 |e 910000PH120706385 |e 910200PH120706385 |k 0/910000/ |k 1/910000/910200/ |p 7 | ||
| 998 | |g 1076334229 |a Lehnert, Thomas |m 1076334229:Lehnert, Thomas |d 910000 |d 910200 |e 910000PL1076334229 |e 910200PL1076334229 |k 0/910000/ |k 1/910000/910200/ |p 6 | ||
| 998 | |g 1022345974 |a Weitz, Jürgen |m 1022345974:Weitz, Jürgen |d 910000 |d 910200 |e 910000PW1022345974 |e 910200PW1022345974 |k 0/910000/ |k 1/910000/910200/ |p 4 | ||
| 998 | |g 1077763433 |a Schwarzbach, Matthias |m 1077763433:Schwarzbach, Matthias |d 910000 |d 910200 |e 910000PS1077763433 |e 910200PS1077763433 |k 0/910000/ |k 1/910000/910200/ |p 3 | ||
| 998 | |g 1021953024 |a Mechtersheimer, Gunhild |m 1021953024:Mechtersheimer, Gunhild |d 910000 |d 912000 |e 910000PM1021953024 |e 912000PM1021953024 |k 0/910000/ |k 1/910000/912000/ |p 2 | ||
| 998 | |g 1084359758 |a Willeke, Frank |m 1084359758:Willeke, Frank |d 910000 |d 910200 |e 910000PW1084359758 |e 910200PW1084359758 |k 0/910000/ |k 1/910000/910200/ |p 1 |x j | ||
| 999 | |a KXP-PPN193747352X |e 4778519558 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"origin":[{"dateIssuedDisp":"1998","dateIssuedKey":"1998"}],"relHost":[{"recId":"266883400","origin":[{"publisherPlace":"Amsterdam [u.a.] ; [Erscheinungsort nicht ermittelbar]","dateIssuedKey":"1992","dateIssuedDisp":"1992-","publisher":"Elsevier ; Pergamon Press"}],"title":[{"title_sort":"European journal of cancer","title":"European journal of cancer"}],"id":{"issn":["1879-0852"],"eki":["266883400"],"zdb":["1468190-0"]},"pubHistory":["28.1992 -"],"note":["Gesehen am 21.03.24","Ungezählte Beil.: Supplement"],"language":["eng"],"part":{"extent":"7","pages":"2087-2093","issue":"13","text":"34(1998), 13, Seite 2087-2093","year":"1998","volume":"34"},"titleAlt":[{"title":"EJC online"}],"corporate":[{"display":"European Organization for Research on Treatment of Cancer","role":"isb"},{"display":"European Association for Cancer Research","role":"isb"},{"display":"European School of Oncology","role":"isb"}],"disp":"Detection of SYT±SSX1/2 fusion transcripts by reverse transcriptase-polymerase chain reaction (RT-PCR) is a valuable diagnostic tool in synovial sarcomaEuropean journal of cancer","type":{"bibl":"periodical","media":"Online-Ressource"}}],"id":{"eki":["193747352X"],"doi":["10.1016/S0959-8049(98)00240-8"]},"physDesc":[{"extent":"7 S."}],"title":[{"title_sort":"Detection of SYT±SSX1/2 fusion transcripts by reverse transcriptase-polymerase chain reaction (RT-PCR) is a valuable diagnostic tool in synovial sarcoma","title":"Detection of SYT±SSX1/2 fusion transcripts by reverse transcriptase-polymerase chain reaction (RT-PCR) is a valuable diagnostic tool in synovial sarcoma"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Online verfügbar 3. Dezember 1998","Gesehen am 01.10.2025"],"recId":"193747352X","person":[{"given":"Frank","display":"Willeke, Frank","role":"aut","family":"Willeke"},{"display":"Mechtersheimer, Gunhild","given":"Gunhild","family":"Mechtersheimer","role":"aut"},{"role":"aut","family":"Schwarzbach","given":"Matthias","display":"Schwarzbach, Matthias"},{"role":"aut","family":"Weitz","display":"Weitz, Jürgen","given":"Jürgen"},{"display":"Zimmer, D.","given":"D.","role":"aut","family":"Zimmer"},{"role":"aut","family":"Lehnert","given":"Thomas","display":"Lehnert, Thomas"},{"display":"Herfarth, Christian","given":"Christian","family":"Herfarth","role":"aut"},{"given":"Magnus von","display":"Knebel Doeberitz, Magnus von","family":"Knebel Doeberitz","role":"aut"},{"role":"aut","family":"Ridder","given":"Rüdiger","display":"Ridder, Rüdiger"}],"language":["eng"],"name":{"displayForm":["F. Willeke, G. Mechtersheimer, M. Schwarzbach, J. Weitz, D. Zimmer, T. Lehnert, Ch. Herfarth, M. von Knebel Doeberitz, R. Ridder"]}} | ||
| SRT | |a WILLEKEFRADETECTIONO1998 | ||