Unravelling the conundrum of nucleolar NR2F1 localization using antibody-based approaches in vitro and in vivo
As a transcription factor, NR2F1 regulates spatiotemporal gene expression in the nucleus particularly during development. Aberrant NR2F1 causes the rare neurodevelopmental disorder Bosch-Boonstra-Schaaf Optic Atrophy Syndrome. In addition, altered NR2F1 expression is frequently observed in various c...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
10 April 2025
|
| In: |
Communications biology
Year: 2025, Volume: 8, Pages: 1-19 |
| ISSN: | 2399-3642 |
| DOI: | 10.1038/s42003-025-07985-1 |
| Online Access: | Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s42003-025-07985-1 Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s42003-025-07985-1 
|
| Author Notes: | Michele Bertacchi, Susanne Theiß, Ayat Ahmed, Michael Eibl, Agnès Loubat, Gwendoline Maharaux, Wanchana Phromkrasae, Krittalak Chakrabandhu, Aylin Camgöz, Marco Antonaci, Christian Patrick Schaaf, Michèle Studer & Magdalena Laugsch |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1937621359 | ||
| 003 | DE-627 | ||
| 005 | 20251120193459.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 251002s2025 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s42003-025-07985-1 |2 doi | |
| 035 | |a (DE-627)1937621359 | ||
| 035 | |a (DE-599)KXP1937621359 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Bertacchi, Michele |e VerfasserIn |0 (DE-588)126057900X |0 (DE-627)1807451399 |4 aut | |
| 245 | 1 | 0 | |a Unravelling the conundrum of nucleolar NR2F1 localization using antibody-based approaches in vitro and in vivo |c Michele Bertacchi, Susanne Theiß, Ayat Ahmed, Michael Eibl, Agnès Loubat, Gwendoline Maharaux, Wanchana Phromkrasae, Krittalak Chakrabandhu, Aylin Camgöz, Marco Antonaci, Christian Patrick Schaaf, Michèle Studer & Magdalena Laugsch |
| 264 | 1 | |c 10 April 2025 | |
| 300 | |b Illustrationen | ||
| 300 | |a 19 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 02.10.2025 | ||
| 520 | |a As a transcription factor, NR2F1 regulates spatiotemporal gene expression in the nucleus particularly during development. Aberrant NR2F1 causes the rare neurodevelopmental disorder Bosch-Boonstra-Schaaf Optic Atrophy Syndrome. In addition, altered NR2F1 expression is frequently observed in various cancers and is considered a prognostic marker or potential therapeutic target. NR2F1 has been found in both the nucleus and nucleoli, suggesting a non-canonical and direct role in the latter compartment. Hence, we studied this phenomenon employing various in vitro and in vivo models using different antibody-dependent approaches. Examination of seven commonly used anti-NR2F1 antibodies in different human cancer and stem cells as well as in wild type and null mice revealed that NR2F1 nucleolar localization is artificial and has no functional role. Our subsequent comparative analysis demonstrated which anti-NR2F1 antibody best fits which approach. The data allow for correct data interpretation and underline the need to optimize any antibody-mediated technique. | ||
| 650 | 4 | |a Cell biology | |
| 650 | 4 | |a Molecular biology | |
| 700 | 1 | |a Theiß, Susanne |e VerfasserIn |0 (DE-588)1170999131 |0 (DE-627)104034660X |0 (DE-576)512750653 |4 aut | |
| 700 | 1 | |a Ahmed, Ayat |e VerfasserIn |0 (DE-588)1378065867 |0 (DE-627)1937624269 |4 aut | |
| 700 | 1 | |a Eibl, Michael |e VerfasserIn |0 (DE-588)1378066529 |0 (DE-627)1937625613 |4 aut | |
| 700 | 1 | |a Loubat, Agnès |e VerfasserIn |4 aut | |
| 700 | 1 | |a Maharaux, Gwendoline |e VerfasserIn |4 aut | |
| 700 | 1 | |a Phromkrasae, Wanchana |e VerfasserIn |4 aut | |
| 700 | 1 | |a Chakrabandhu, Krittalak |e VerfasserIn |4 aut | |
| 700 | 1 | |a Camgöz, Aylin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Antonaci, Marco |e VerfasserIn |0 (DE-588)1378068904 |0 (DE-627)1937628434 |4 aut | |
| 700 | 1 | |a Schaaf, Christian P. |d 1978- |e VerfasserIn |0 (DE-588)130397318 |0 (DE-627)500686025 |0 (DE-576)298170345 |4 aut | |
| 700 | 1 | |a Studer, Michèle |e VerfasserIn |4 aut | |
| 700 | 1 | |a Laugsch, Magdalena |d 1968- |e VerfasserIn |0 (DE-588)129620122 |0 (DE-627)475208722 |0 (DE-576)297752081 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Communications biology |d London : Springer Nature, 2018 |g 8(2025), Artikel-ID 594, Seite 1-19 |h Online-Ressource |w (DE-627)101369080X |w (DE-600)2919698-X |w (DE-576)499739132 |x 2399-3642 |7 nnas |a Unravelling the conundrum of nucleolar NR2F1 localization using antibody-based approaches in vitro and in vivo |
| 773 | 1 | 8 | |g volume:8 |g year:2025 |g elocationid:594 |g pages:1-19 |g extent:19 |a Unravelling the conundrum of nucleolar NR2F1 localization using antibody-based approaches in vitro and in vivo |
| 856 | 4 | 0 | |u https://doi.org/10.1038/s42003-025-07985-1 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/s42003-025-07985-1 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20251002 | ||
| 993 | |a Article | ||
| 994 | |a 2025 | ||
| 998 | |g 129620122 |a Laugsch, Magdalena |m 129620122:Laugsch, Magdalena |d 910000 |d 911500 |e 910000PL129620122 |e 911500PL129620122 |k 0/910000/ |k 1/910000/911500/ |p 13 |y j | ||
| 998 | |g 130397318 |a Schaaf, Christian P. |m 130397318:Schaaf, Christian P. |d 910000 |d 911500 |e 910000PS130397318 |e 911500PS130397318 |k 0/910000/ |k 1/910000/911500/ |p 11 | ||
| 998 | |g 1378068904 |a Antonaci, Marco |m 1378068904:Antonaci, Marco |p 10 | ||
| 998 | |g 1378066529 |a Eibl, Michael |m 1378066529:Eibl, Michael |p 4 | ||
| 998 | |g 1378065867 |a Ahmed, Ayat |m 1378065867:Ahmed, Ayat |p 3 | ||
| 998 | |g 1170999131 |a Theiß, Susanne |m 1170999131:Theiß, Susanne |d 50000 |e 50000PT1170999131 |k 0/50000/ |p 2 | ||
| 999 | |a KXP-PPN1937621359 |e 4779885736 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"language":["eng"],"recId":"1937621359","note":["Gesehen am 02.10.2025"],"origin":[{"dateIssuedKey":"2025","dateIssuedDisp":"10 April 2025"}],"name":{"displayForm":["Michele Bertacchi, Susanne Theiß, Ayat Ahmed, Michael Eibl, Agnès Loubat, Gwendoline Maharaux, Wanchana Phromkrasae, Krittalak Chakrabandhu, Aylin Camgöz, Marco Antonaci, Christian Patrick Schaaf, Michèle Studer & Magdalena Laugsch"]},"id":{"doi":["10.1038/s42003-025-07985-1"],"eki":["1937621359"]},"type":{"bibl":"article-journal","media":"Online-Ressource"},"relHost":[{"part":{"volume":"8","text":"8(2025), Artikel-ID 594, Seite 1-19","year":"2025","extent":"19","pages":"1-19"},"language":["eng"],"recId":"101369080X","origin":[{"dateIssuedDisp":"[2018]-","publisher":"Springer Nature","publisherPlace":"London"}],"disp":"Unravelling the conundrum of nucleolar NR2F1 localization using antibody-based approaches in vitro and in vivoCommunications biology","pubHistory":["1 (2018)-"],"id":{"eki":["101369080X"],"issn":["2399-3642"],"zdb":["2919698-X"]},"type":{"media":"Online-Ressource","bibl":"periodical"},"title":[{"title":"Communications biology","title_sort":"Communications biology"}],"physDesc":[{"extent":"Online-Ressource"}]}],"physDesc":[{"extent":"19 S.","noteIll":"Illustrationen"}],"title":[{"title":"Unravelling the conundrum of nucleolar NR2F1 localization using antibody-based approaches in vitro and in vivo","title_sort":"Unravelling the conundrum of nucleolar NR2F1 localization using antibody-based approaches in vitro and in vivo"}],"person":[{"display":"Bertacchi, Michele","family":"Bertacchi","given":"Michele","role":"aut"},{"role":"aut","given":"Susanne","family":"Theiß","display":"Theiß, Susanne"},{"display":"Ahmed, Ayat","family":"Ahmed","given":"Ayat","role":"aut"},{"display":"Eibl, Michael","family":"Eibl","given":"Michael","role":"aut"},{"family":"Loubat","display":"Loubat, Agnès","role":"aut","given":"Agnès"},{"role":"aut","given":"Gwendoline","family":"Maharaux","display":"Maharaux, Gwendoline"},{"display":"Phromkrasae, Wanchana","family":"Phromkrasae","given":"Wanchana","role":"aut"},{"family":"Chakrabandhu","display":"Chakrabandhu, Krittalak","role":"aut","given":"Krittalak"},{"family":"Camgöz","display":"Camgöz, Aylin","role":"aut","given":"Aylin"},{"given":"Marco","role":"aut","display":"Antonaci, Marco","family":"Antonaci"},{"given":"Christian P.","role":"aut","display":"Schaaf, Christian P.","family":"Schaaf"},{"display":"Studer, Michèle","family":"Studer","given":"Michèle","role":"aut"},{"family":"Laugsch","display":"Laugsch, Magdalena","role":"aut","given":"Magdalena"}]} | ||
| SRT | |a BERTACCHIMUNRAVELLIN1020 | ||