Methotrexate-loaded liposomal formulation enables 6-week sustained intraocular therapeutic drug release in a porcine model
Methotrexate (MTX) inhibits cell proliferation, which underlies ocular diseases, including intraocular lymphoma and proliferative vitreoretinopathy. However, MTX normally requires bi-weekly intravitreal injections due to a short half-life, causing rapid clearance below therapeutic thresholds within...
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| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2025
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| In: |
Advanced healthcare materials
Year: 2025, Pages: 1-12 |
| ISSN: | 2192-2659 |
| DOI: | 10.1002/adhm.202503230 |
| Online Access: | Verlag, kostenfrei, Volltext: https://doi.org/10.1002/adhm.202503230 Verlag, kostenfrei, Volltext: http://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.202503230 
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| Author Notes: | Maximilian Hammer, Lea Skrzypczyk, Sabrina Wohlfart, Bryan Calder Ackermann, Ludwig Geisweid, Simon William Pohl, Margarita Karaivanova, Jonathan Herth, Victor Aristide Augustin, Alexander Studier-Fischer, Tina Sackmann, Leon Kaulen, Anna Steyer, Walter Mier, David H Steel, Kanmin Xue, Gerd Uwe Auffarth, and Philipp Uhl |
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| 245 | 1 | 0 | |a Methotrexate-loaded liposomal formulation enables 6-week sustained intraocular therapeutic drug release in a porcine model |c Maximilian Hammer, Lea Skrzypczyk, Sabrina Wohlfart, Bryan Calder Ackermann, Ludwig Geisweid, Simon William Pohl, Margarita Karaivanova, Jonathan Herth, Victor Aristide Augustin, Alexander Studier-Fischer, Tina Sackmann, Leon Kaulen, Anna Steyer, Walter Mier, David H Steel, Kanmin Xue, Gerd Uwe Auffarth, and Philipp Uhl |
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| 520 | |a Methotrexate (MTX) inhibits cell proliferation, which underlies ocular diseases, including intraocular lymphoma and proliferative vitreoretinopathy. However, MTX normally requires bi-weekly intravitreal injections due to a short half-life, causing rapid clearance below therapeutic thresholds within 72h. To overcome these limitations, sustained-release carriers, including poly(lactic-co-glycolic) acid-based implants, were investigated in vitro previously. These systems offer prolonged drug delivery but require relatively large-gauge surgical implantation, which increases the risk of surgical complications and limits their practical use. In this study, MTX-loaded liposomes that can be administered via a 30-gauge cannula are developed, obviating the need for more invasive surgical implantation. This phospholipid-based liposomal formulation succeeded in enabling sustained methotrexate release at therapeutic levels for over six weeks following a single intravitreal injection, demonstrated in vivo in a large animal pig model. High biocompatibility of this novel liposomal formulation is confirmed through longitudinal retinal structure (optical coherence tomography) and function (electroretinography) assessments. This liposomal formulation of MTX provides a clinically and surgically optimized drug delivery system that allows improved management of intraocular lymphoma and proliferative vitreoretinopathy in the future. | ||
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| 650 | 4 | |a methotrexate | |
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| 650 | 4 | |a sustained drug release | |
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