H/ACA snR30 snoRNP guides independent 18S rRNA subdomain formation
Ribosome biogenesis follows a cascade of pre-rRNA folding and processing steps, coordinated with ribosomal protein incorporation. Nucleolar 90S pre-ribosomes are well-described stable intermediates, composed of pre-18S rRNA, ribosomal S-proteins, U3 snoRNA, and ~70 assembly factors. However, how num...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
21 May 2025
|
| In: |
Nature Communications
Year: 2025, Jahrgang: 16, Pages: 1-15 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-025-59656-8 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41467-025-59656-8 Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41467-025-59656-8 
|
| Verfasserangaben: | Paulina Fischer, Matthias Thoms, Benjamin Lau, Timo Denk, Maria Kuvshinova, Otto Berninghausen, Dirk Flemming, Ed Hurt & Roland Beckmann |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 193908072X | ||
| 003 | DE-627 | ||
| 005 | 20251127235834.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 251022s2025 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s41467-025-59656-8 |2 doi | |
| 035 | |a (DE-627)193908072X | ||
| 035 | |a (DE-599)KXP193908072X | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Fischer, Paulina |d 1987- |e VerfasserIn |0 (DE-588)1131652533 |0 (DE-627)886286670 |0 (DE-576)488349559 |4 aut | |
| 245 | 1 | 0 | |a H/ACA snR30 snoRNP guides independent 18S rRNA subdomain formation |c Paulina Fischer, Matthias Thoms, Benjamin Lau, Timo Denk, Maria Kuvshinova, Otto Berninghausen, Dirk Flemming, Ed Hurt & Roland Beckmann |
| 264 | 1 | |c 21 May 2025 | |
| 300 | |b Illustrationen, Diagramme | ||
| 300 | |a 15 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 22.10.2025 | ||
| 520 | |a Ribosome biogenesis follows a cascade of pre-rRNA folding and processing steps, coordinated with ribosomal protein incorporation. Nucleolar 90S pre-ribosomes are well-described stable intermediates, composed of pre-18S rRNA, ribosomal S-proteins, U3 snoRNA, and ~70 assembly factors. However, how numerous snoRNAs control pre-rRNA modification and folding during early maturation events remains unclear. We identify snR30 (human U17), the only essential H/ACA snoRNA in yeast, which binds with Cbf5-Gar1-Nop10-Nhp2 to a pre-18S rRNA subdomain containing platform helices and ES6 of the 40S central domain. Integration into the 90S is blocked by RNA hybridization with snR30. The snoRNP complex coordinates the recruitment of early assembly factors Krr1-Utp23-Kri1 and ribosomal proteins uS11-uS15, enabling isolated subdomain assembly. Krr1-dependent release of snR30 culminates in integration of the platform into the 90S. Our study reveals the essential role of snR30 in chaperoning central domain formation as a discrete assembly unit externalized from the pre-ribosomal core. | ||
| 650 | 4 | |a Cryoelectron microscopy | |
| 650 | 4 | |a RNA | |
| 700 | 1 | |a Thoms, Matthias |e VerfasserIn |4 aut | |
| 700 | 1 | |a Lau, Benjamin |d 1980- |e VerfasserIn |0 (DE-588)1197332979 |0 (DE-627)167911915X |4 aut | |
| 700 | 1 | |a Denk, Timo |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kuvshinova, Maria |e VerfasserIn |4 aut | |
| 700 | 1 | |8 1\p |a Berninghausen, Otto |d 1964- |e VerfasserIn |0 (DE-588)120952629 |0 (DE-627)080991173 |0 (DE-576)292465548 |4 aut | |
| 700 | 1 | |a Flemming, Dirk |e VerfasserIn |0 (DE-588)1070679046 |0 (DE-627)824448596 |0 (DE-576)432207961 |4 aut | |
| 700 | 1 | |a Hurt, Ed |d 1955- |e VerfasserIn |0 (DE-588)11021952X |0 (DE-627)729291863 |0 (DE-576)373308574 |4 aut | |
| 700 | 1 | |a Beckmann, Roland |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nature Communications |d [London] : Springer Nature, 2010 |g 16(2025), Artikel-ID 4720, Seite 1-15 |h Online-Ressource |w (DE-627)626457688 |w (DE-600)2553671-0 |w (DE-576)331555905 |x 2041-1723 |7 nnas |a H/ACA snR30 snoRNP guides independent 18S rRNA subdomain formation |
| 773 | 1 | 8 | |g volume:16 |g year:2025 |g elocationid:4720 |g pages:1-15 |g extent:15 |a H/ACA snR30 snoRNP guides independent 18S rRNA subdomain formation |
| 856 | 4 | 0 | |u https://doi.org/10.1038/s41467-025-59656-8 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/s41467-025-59656-8 |x Verlag |z kostenfrei |3 Volltext |
| 883 | |8 1\p |a cgwrk |d 20251101 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 951 | |a AR | ||
| 992 | |a 20251022 | ||
| 993 | |a Article | ||
| 994 | |a 2025 | ||
| 998 | |g 11021952X |a Hurt, Ed |m 11021952X:Hurt, Ed |d 700000 |d 711000 |e 700000PH11021952X |e 711000PH11021952X |k 0/700000/ |k 1/700000/711000/ |p 8 | ||
| 998 | |g 1070679046 |a Flemming, Dirk |m 1070679046:Flemming, Dirk |d 700000 |d 711000 |e 700000PF1070679046 |e 711000PF1070679046 |k 0/700000/ |k 1/700000/711000/ |p 7 | ||
| 998 | |g 1197332979 |a Lau, Benjamin |m 1197332979:Lau, Benjamin |d 700000 |d 711000 |e 700000PL1197332979 |e 711000PL1197332979 |k 0/700000/ |k 1/700000/711000/ |p 3 | ||
| 998 | |g 1131652533 |a Fischer, Paulina |m 1131652533:Fischer, Paulina |d 700000 |d 711000 |e 700000PF1131652533 |e 711000PF1131652533 |k 0/700000/ |k 1/700000/711000/ |p 1 |x j | ||
| 999 | |a KXP-PPN193908072X |e 4790516040 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"language":["eng"],"title":[{"title_sort":"H/ACA snR30 snoRNP guides independent 18S rRNA subdomain formation","title":"H/ACA snR30 snoRNP guides independent 18S rRNA subdomain formation"}],"relHost":[{"pubHistory":["2010-"],"part":{"year":"2025","text":"16(2025), Artikel-ID 4720, Seite 1-15","volume":"16","pages":"1-15","extent":"15"},"language":["eng"],"title":[{"title_sort":"Nature Communications","title":"Nature Communications"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"recId":"626457688","origin":[{"dateIssuedDisp":"[2010]-","publisher":"Springer Nature ; Nature Publishing Group UK","publisherPlace":"[London] ; [London]"}],"note":["Gesehen am 13.06.24"],"disp":"H/ACA snR30 snoRNP guides independent 18S rRNA subdomain formationNature Communications","physDesc":[{"extent":"Online-Ressource"}],"id":{"eki":["626457688"],"issn":["2041-1723"],"zdb":["2553671-0"]}}],"note":["Gesehen am 22.10.2025"],"physDesc":[{"extent":"15 S.","noteIll":"Illustrationen, Diagramme"}],"id":{"eki":["193908072X"],"doi":["10.1038/s41467-025-59656-8"]},"type":{"bibl":"article-journal","media":"Online-Ressource"},"recId":"193908072X","origin":[{"dateIssuedDisp":"21 May 2025","dateIssuedKey":"2025"}],"person":[{"display":"Fischer, Paulina","role":"aut","given":"Paulina","family":"Fischer"},{"display":"Thoms, Matthias","role":"aut","given":"Matthias","family":"Thoms"},{"family":"Lau","given":"Benjamin","role":"aut","display":"Lau, Benjamin"},{"given":"Timo","role":"aut","display":"Denk, Timo","family":"Denk"},{"family":"Kuvshinova","display":"Kuvshinova, Maria","role":"aut","given":"Maria"},{"display":"Berninghausen, Otto","role":"aut","given":"Otto","family":"Berninghausen"},{"family":"Flemming","given":"Dirk","role":"aut","display":"Flemming, Dirk"},{"family":"Hurt","display":"Hurt, Ed","given":"Ed","role":"aut"},{"family":"Beckmann","role":"aut","given":"Roland","display":"Beckmann, Roland"}],"name":{"displayForm":["Paulina Fischer, Matthias Thoms, Benjamin Lau, Timo Denk, Maria Kuvshinova, Otto Berninghausen, Dirk Flemming, Ed Hurt & Roland Beckmann"]}} | ||
| SRT | |a FISCHERPAUHACASNR30S2120 | ||