Isolation, characterization, and proteomic analysis of plasma-derived extracellular vesicles for cardiovascular biomarker discovery
Extracellular vesicles (EV) are cell-derived, lipid bilayer-enclosed, non-replicable nanoparticles. EV currently gain attention in cardiovascular research due to their role in regulating intercellular communication, potentially serving as valuable biomarkers for cardiovascular disease. However, the...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) Video |
| Sprache: | Englisch |
| Veröffentlicht: |
January 31st, 2025
|
| In: |
JoVE. Video journal
Year: 2025, Heft: 215, Pages: ? |
| ISSN: | 1940-087X |
| DOI: | 10.3791/67083 |
| Schlagworte: | |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3791/67083 Verlag, lizenzpflichtig, Volltext: https://app.jove.com/t/67083/isolation-characterization-proteomic-analysis-plasma-derived 
|
| Verfasserangaben: | Gabriel Jakob Trauner, Dominika Bernath-Nagy, Melek Sükran Kalinyaprak, Sabine Merker, Marcin Luzarowski, Florian Leuschner, Norbert Frey, Evangelos Giannitsis, Jona Benjamin Krohn |
MARC
| LEADER | 00000cga a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1939333350 | ||
| 003 | DE-627 | ||
| 005 | 20251211091559.0 | ||
| 006 | m o | | | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 251023s2025 xx ||| | o ||eng c | ||
| 024 | 7 | |a 10.3791/67083 |2 doi | |
| 035 | |a (DE-627)1939333350 | ||
| 035 | |a (DE-599)KXP1939333350 | ||
| 035 | |a (OCoLC)1559713326 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Trauner, Gabriel Jakob |e VerfasserIn |0 (DE-588)1383953376 |0 (DE-627)1945755113 |4 aut | |
| 245 | 1 | 0 | |a Isolation, characterization, and proteomic analysis of plasma-derived extracellular vesicles for cardiovascular biomarker discovery |c Gabriel Jakob Trauner, Dominika Bernath-Nagy, Melek Sükran Kalinyaprak, Sabine Merker, Marcin Luzarowski, Florian Leuschner, Norbert Frey, Evangelos Giannitsis, Jona Benjamin Krohn |
| 264 | 1 | |c January 31st, 2025 | |
| 300 | |a 1 Online-Ressource (1 Videodatei, 5:30 min) |b farbig | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 336 | |a zweidimensionales bewegtes Bild |b tdi |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Enthält auch Textversionen | ||
| 500 | |a Gesehen am 23.10.2025 | ||
| 500 | |a Wissenschaftlicher Film. Deutschland. 2025 | ||
| 520 | |a Extracellular vesicles (EV) are cell-derived, lipid bilayer-enclosed, non-replicable nanoparticles. EV currently gain attention in cardiovascular research due to their role in regulating intercellular communication, potentially serving as valuable biomarkers for cardiovascular disease. However, the EV proteome and its potential as a biomarker in cardiovascular diagnostics remain poorly understood. This protocol presents a standardized method for the isolation and quantification of plasma-derived EV and the analysis of their protein cargo using plasma samples from patients presenting to the Chest Pain Unit of a large university hospital. Following routine phlebotomy, EV are isolated from plasma by differential ultracentrifugation. The enrichment of specific EV marker proteins in EV isolates is visualized by immunoblotting, and average size distribution and plasma EV concentrations are quantified by nanoparticle tracking analysis. Finally, ultra-performance liquid chromatography-tandem mass spectrometry is employed for label-free analysis of the EV proteome. This protocol thus provides a comprehensive approach to study and use plasma-derived EV as potential carriers of critical biological information as well as to explore their potential as novel biomarkers. | ||
| 655 | 7 | |a Film |0 (DE-588)4017102-4 |0 (DE-627)104559683 |0 (DE-576)208918531 |2 gnd-content | |
| 700 | 1 | |a Bernáth-Nagy, Dominika |e VerfasserIn |0 (DE-588)1360702180 |0 (DE-627)1920204121 |4 aut | |
| 700 | 1 | |a Kalinyaprak, Melek Sükran |e VerfasserIn |4 aut | |
| 700 | 1 | |a Merker, Sabine |e VerfasserIn |0 (DE-588)103825017X |0 (DE-627)756832381 |0 (DE-576)392231964 |4 aut | |
| 700 | 1 | |a Luzarowski, Marcin |e VerfasserIn |0 (DE-588)1196882940 |0 (DE-627)1678733911 |4 aut | |
| 700 | 1 | |a Leuschner, Florian |e VerfasserIn |0 (DE-588)1072736144 |0 (DE-627)828165742 |0 (DE-576)189305347 |4 aut | |
| 700 | 1 | |a Frey, Norbert |e VerfasserIn |0 (DE-588)141244976 |0 (DE-627)625824075 |0 (DE-576)322969514 |4 aut | |
| 700 | 1 | |a Giannitsis, Evangelos |e VerfasserIn |0 (DE-588)113336241 |0 (DE-627)577184091 |0 (DE-576)289759579 |4 aut | |
| 700 | 1 | |a Krohn, Jona B. |d 1988- |e VerfasserIn |0 (DE-588)113752880X |0 (DE-627)894765272 |0 (DE-576)491446411 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t JoVE. Video journal |d [Erscheinungsort nicht ermittelbar] : [Verlag nicht ermittelbar], 2006 |g (2025), 215, Artikel-ID e67083, Seite ? |h Online-Ressource |w (DE-627)521454778 |w (DE-600)2259946-0 |w (DE-576)338522980 |x 1940-087X |7 nngs |a Isolation, characterization, and proteomic analysis of plasma-derived extracellular vesicles for cardiovascular biomarker discovery |
| 773 | 1 | 8 | |g year:2025 |g number:215 |g elocationid:e67083 |g pages:? |a Isolation, characterization, and proteomic analysis of plasma-derived extracellular vesicles for cardiovascular biomarker discovery |
| 856 | 4 | 0 | |u https://doi.org/10.3791/67083 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |7 1 |
| 856 | 4 | 0 | |u https://app.jove.com/t/67083/isolation-characterization-proteomic-analysis-plasma-derived |x Verlag |z lizenzpflichtig |3 Volltext |7 1 |
| 935 | |c vide | ||
| 937 | |a Wissenschaftlicher Film |b Deutschland |c 2025 | ||
| 951 | |a AR | ||
| 992 | |a 20251023 | ||
| 993 | |a Video | ||
| 994 | |a 2025 | ||
| 998 | |g 113752880X |a Krohn, Jona B. |m 113752880X:Krohn, Jona B. |d 910000 |d 910100 |e 910000PK113752880X |e 910100PK113752880X |k 0/910000/ |k 1/910000/910100/ |p 9 |y j | ||
| 998 | |g 113336241 |a Giannitsis, Evangelos |m 113336241:Giannitsis, Evangelos |d 910000 |d 910100 |d 50000 |e 910000PG113336241 |e 910100PG113336241 |e 50000PG113336241 |k 0/910000/ |k 1/910000/910100/ |k 0/50000/ |p 8 | ||
| 998 | |g 141244976 |a Frey, Norbert |m 141244976:Frey, Norbert |d 910000 |d 910100 |e 910000PF141244976 |e 910100PF141244976 |k 0/910000/ |k 1/910000/910100/ |p 7 | ||
| 998 | |g 1072736144 |a Leuschner, Florian |m 1072736144:Leuschner, Florian |d 910000 |d 910100 |e 910000PL1072736144 |e 910100PL1072736144 |k 0/910000/ |k 1/910000/910100/ |p 6 | ||
| 998 | |g 1196882940 |a Luzarowski, Marcin |m 1196882940:Luzarowski, Marcin |d 700000 |d 706000 |e 700000PL1196882940 |e 706000PL1196882940 |k 0/700000/ |k 1/700000/706000/ |p 5 | ||
| 998 | |g 103825017X |a Merker, Sabine |m 103825017X:Merker, Sabine |d 700000 |d 706000 |e 700000PM103825017X |e 706000PM103825017X |k 0/700000/ |k 1/700000/706000/ |p 4 | ||
| 999 | |a KXP-PPN1939333350 |e 4790936546 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1939333350","person":[{"given":"Gabriel Jakob","display":"Trauner, Gabriel Jakob","family":"Trauner","role":"aut"},{"given":"Dominika","display":"Bernáth-Nagy, Dominika","family":"Bernáth-Nagy","role":"aut"},{"display":"Kalinyaprak, Melek Sükran","given":"Melek Sükran","family":"Kalinyaprak","role":"aut"},{"role":"aut","family":"Merker","given":"Sabine","display":"Merker, Sabine"},{"family":"Luzarowski","role":"aut","given":"Marcin","display":"Luzarowski, Marcin"},{"role":"aut","family":"Leuschner","given":"Florian","display":"Leuschner, Florian"},{"family":"Frey","role":"aut","given":"Norbert","display":"Frey, Norbert"},{"display":"Giannitsis, Evangelos","given":"Evangelos","family":"Giannitsis","role":"aut"},{"role":"aut","family":"Krohn","given":"Jona B.","display":"Krohn, Jona B."}],"note":["Enthält auch Textversionen","Gesehen am 23.10.2025","Wissenschaftlicher Film. Deutschland. 2025"],"language":["eng"],"name":{"displayForm":["Gabriel Jakob Trauner, Dominika Bernath-Nagy, Melek Sükran Kalinyaprak, Sabine Merker, Marcin Luzarowski, Florian Leuschner, Norbert Frey, Evangelos Giannitsis, Jona Benjamin Krohn"]},"relHost":[{"origin":[{"publisher":"[Verlag nicht ermittelbar]","dateIssuedDisp":"2006-","dateIssuedKey":"2006","publisherPlace":"[Erscheinungsort nicht ermittelbar]"}],"title":[{"title_sort":"JoVE","partname":"Video journal","title":"JoVE"}],"physDesc":[{"extent":"Online-Ressource"}],"id":{"issn":["1940-087X"],"eki":["521454778"],"zdb":["2259946-0"]},"recId":"521454778","titleAlt":[{"title":"JoVE journal"},{"title":"JoVE journal Biology"},{"title":"JoVE journal Medicine"},{"title":"JoVE journal Behavior"},{"title":"JoVE journal Biochemistry"},{"title":"JoVE journal Bioengineering"},{"title":"JoVE journal Cancer research"},{"title":"JoVE journal Chemistry"},{"title":"JoVE journal Developmental biology"},{"title":"JoVE journal Engineering"},{"title":"JoVE journal Environment"},{"title":"JoVE journal Genetics"},{"title":"JoVE journal Immunology and infection"},{"title":"JoVE journal Neuroscience"},{"title":"Journal of visualized experiments"}],"disp":"Isolation, characterization, and proteomic analysis of plasma-derived extracellular vesicles for cardiovascular biomarker discoveryJoVE. Video journal","type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 10.02.25","Inhaltliche Gliederung in: Behavior; Biochemistry; Bioengineering; Biology; Cancer research; Chemistry; Developmental biology; Engineering; Environment; Genetics; Immunology and infection; Medicine; Neuroscience"],"pubHistory":["1.2006 -"],"language":["eng"],"part":{"year":"2025","text":"(2025), 215, Artikel-ID e67083, Seite ?","pages":"?","issue":"215"}}],"origin":[{"dateIssuedKey":"2025","dateIssuedDisp":"January 31st, 2025"}],"title":[{"title":"Isolation, characterization, and proteomic analysis of plasma-derived extracellular vesicles for cardiovascular biomarker discovery","title_sort":"Isolation, characterization, and proteomic analysis of plasma-derived extracellular vesicles for cardiovascular biomarker discovery"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"id":{"doi":["10.3791/67083"],"eki":["1939333350"]},"physDesc":[{"extent":"1 Online-Ressource (1 Videodatei, 5:30 min) S.","noteIll":"farbig"}]} | ||
| SRT | |a TRAUNERGABISOLATIONC3120 | ||