Morphology-dependent entry kinetics and spread of influenza A virus
Influenza A viruses (IAV) display a broad variety of morphologies ranging from spherical to long filamentous virus particles. These diverse phenotypes are believed to allow the virus to overcome various immunological and pulmonary barriers during entry into the airway epithelium, and to influence th...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
Jul 2025
|
| In: |
The EMBO journal
Year: 2025, Jahrgang: 44, Heft: 14, Pages: 3959-3982 |
| ISSN: | 1460-2075 |
| DOI: | 10.1038/s44318-025-00481-6 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s44318-025-00481-6 Verlag, kostenfrei, Volltext: https://www.embopress.org/doi/full/10.1038/s44318-025-00481-6 
|
| Verfasserangaben: | Sarah Peterl, Carmen M. Lahr, Carl N. Schneider, Janis Meyer, Xenia Podlipensky, Vera Lechner, Maria Villiou, Larissa Eis, Steffen Klein, Charlotta Funaya, Elisabetta Ada Cavalcanti-Adam, Frederik Graw, Christine Selhuber-Unkel, Karl Rohr & Petr Chlanda |
MARC
| LEADER | 00000naa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1939454344 | ||
| 003 | DE-627 | ||
| 005 | 20251027073412.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 251027s2025 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s44318-025-00481-6 |2 doi | |
| 035 | |a (DE-627)1939454344 | ||
| 035 | |a (DE-599)KXP1939454344 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Peterl, Sarah |e VerfasserIn |0 (DE-588)1379958741 |0 (DE-627)1939454816 |4 aut | |
| 245 | 1 | 0 | |a Morphology-dependent entry kinetics and spread of influenza A virus |c Sarah Peterl, Carmen M. Lahr, Carl N. Schneider, Janis Meyer, Xenia Podlipensky, Vera Lechner, Maria Villiou, Larissa Eis, Steffen Klein, Charlotta Funaya, Elisabetta Ada Cavalcanti-Adam, Frederik Graw, Christine Selhuber-Unkel, Karl Rohr & Petr Chlanda |
| 264 | 1 | |c Jul 2025 | |
| 300 | |b Illustrationen, Diagramme | ||
| 300 | |a 24 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Online veröffentlicht: 9 June 2025 | ||
| 500 | |a Gesehen am 27.10.2025 | ||
| 520 | |a Influenza A viruses (IAV) display a broad variety of morphologies ranging from spherical to long filamentous virus particles. These diverse phenotypes are believed to allow the virus to overcome various immunological and pulmonary barriers during entry into the airway epithelium, and to influence the viral entry pathway. Notably, laboratory-adapted IAV strains predominantly adopt a spherical form, yet the factors driving this preference as well as the factors favoring filamentous morphology in physiological settings remain unclear. To address this, we generated fluorescent reporter viruses with identical surface glycoproteins but distinct morphologies and developed a correlative light and scanning electron microscopy workflow. This enabled us to investigate the impact of viral morphology on spread, and to identify conditions favoring either form. Our findings demonstrate that filamentous IAV spread significantly slower in various cell lines, consistent with delayed entry kinetics and in-cell cryo-electron tomography, explaining the predominance of spherical forms in laboratory-adapted strains. Cellular junction integrity, neuraminidase activity, and mucin do not inhibit IAV spread in a morphology-dependent manner. However, filamentous virions confer a selective advantage under neutralizing-antibody pressure against hemagglutinin. | ||
| 650 | 4 | |a A(H5N1) VIRUS | |
| 650 | 4 | |a CELLS | |
| 650 | 4 | |a CLEM | |
| 650 | 4 | |a Filamentous influenza A virus | |
| 650 | 4 | |a FILAMENTOUS MORPHOLOGY | |
| 650 | 4 | |a In situ cryo-ET | |
| 650 | 4 | |a INHIBITION | |
| 650 | 4 | |a M1 | |
| 650 | 4 | |a M2 PROTEINS | |
| 650 | 4 | |a MICROSCOPY | |
| 650 | 4 | |a Mucin | |
| 650 | 4 | |a NEURAMINIDASE | |
| 650 | 4 | |a Neutralizing antibodies | |
| 650 | 4 | |a TOMOGRAPHY | |
| 650 | 4 | |a TRANSMISSION | |
| 700 | 1 | |a Lahr, Carmen M. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schneider, Carl N. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Meyer, Janis |d 1994- |e VerfasserIn |0 (DE-588)1309865612 |0 (DE-627)187026441X |4 aut | |
| 700 | 1 | |a Podlipensky, Xenia |e VerfasserIn |0 (DE-588)1379958776 |0 (DE-627)1939454948 |4 aut | |
| 700 | 1 | |a Lechner, Vera |e VerfasserIn |0 (DE-588)1379958822 |0 (DE-627)193945509X |4 aut | |
| 700 | 1 | |a Villiou, Maria |e VerfasserIn |4 aut | |
| 700 | 1 | |a Eis, Larissa |e VerfasserIn |4 aut | |
| 700 | 1 | |a Klein, Steffen |e VerfasserIn |4 aut | |
| 700 | 1 | |a Funaya, Charlotta |e VerfasserIn |4 aut | |
| 700 | 1 | |a Cavalcanti-Adam, Elisabetta A. |d 1972- |e VerfasserIn |0 (DE-588)1059179458 |0 (DE-627)798132256 |0 (DE-576)189133511 |4 aut | |
| 700 | 1 | |a Graw, Frederik |e VerfasserIn |4 aut | |
| 700 | 1 | |a Selhuber-Unkel, Christine |d 1980- |e VerfasserIn |0 (DE-588)132414759 |0 (DE-627)522682626 |0 (DE-576)299131491 |4 aut | |
| 700 | 1 | |a Rohr, Karl |e VerfasserIn |0 (DE-588)137474466 |0 (DE-627)695829440 |0 (DE-576)303788593 |4 aut | |
| 700 | 1 | |a Chlanda, Petr |e VerfasserIn |0 (DE-588)141254823 |0 (DE-627)626165261 |0 (DE-576)323011152 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |a European Molecular Biology Organization |t The EMBO journal |d [London] : Nature Publishing Group UK, 1982 |g 44(2025), 14 vom: Juli, Seite 3959-3982 |h Online-Ressource |w (DE-627)266022529 |w (DE-600)1467419-1 |w (DE-576)075961377 |x 1460-2075 |7 nnas |
| 773 | 1 | 8 | |g volume:44 |g year:2025 |g number:14 |g month:07 |g pages:3959-3982 |g extent:24 |a Morphology-dependent entry kinetics and spread of influenza A virus |
| 856 | 4 | 0 | |u https://doi.org/10.1038/s44318-025-00481-6 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.embopress.org/doi/full/10.1038/s44318-025-00481-6 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20251027 | ||
| 993 | |a Article | ||
| 994 | |a 2025 | ||
| 998 | |g 141254823 |a Chlanda, Petr |m 141254823:Chlanda, Petr |d 910000 |d 911700 |e 910000PC141254823 |e 911700PC141254823 |k 0/910000/ |k 1/910000/911700/ |p 15 |y j | ||
| 998 | |g 137474466 |a Rohr, Karl |m 137474466:Rohr, Karl |d 160000 |d 160100 |d 160000 |e 160000PR137474466 |e 160100PR137474466 |e 160000PR137474466 |k 0/160000/ |k 1/160000/160100/ |k 0/160000/ |p 14 | ||
| 998 | |g 132414759 |a Selhuber-Unkel, Christine |m 132414759:Selhuber-Unkel, Christine |d 160000 |d 160200 |e 160000PS132414759 |e 160200PS132414759 |k 0/160000/ |k 1/160000/160200/ |p 13 | ||
| 998 | |g 1059179458 |a Cavalcanti-Adam, Elisabetta A. |m 1059179458:Cavalcanti-Adam, Elisabetta A. |d 120000 |e 120000PC1059179458 |k 0/120000/ |p 11 | ||
| 998 | |g 1379958822 |a Lechner, Vera |m 1379958822:Lechner, Vera |p 6 | ||
| 998 | |g 1379958776 |a Podlipensky, Xenia |m 1379958776:Podlipensky, Xenia |p 4 | ||
| 998 | |g 1309865612 |a Meyer, Janis |m 1309865612:Meyer, Janis |d 910000 |d 911700 |e 910000PM1309865612 |e 911700PM1309865612 |k 0/910000/ |k 1/910000/911700/ |p 3 | ||
| 998 | |g 1379958741 |a Peterl, Sarah |m 1379958741:Peterl, Sarah |d 910000 |d 911700 |e 910000PP1379958741 |e 911700PP1379958741 |k 0/910000/ |k 1/910000/911700/ |p 1 |x j | ||
| 999 | |a KXP-PPN1939454344 |e 4791861558 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"family":"Peterl","given":"Sarah","display":"Peterl, Sarah","role":"aut"},{"family":"Lahr","given":"Carmen M.","display":"Lahr, Carmen M.","role":"aut"},{"given":"Carl N.","family":"Schneider","role":"aut","display":"Schneider, Carl N."},{"family":"Meyer","given":"Janis","display":"Meyer, Janis","role":"aut"},{"family":"Podlipensky","given":"Xenia","display":"Podlipensky, Xenia","role":"aut"},{"given":"Vera","family":"Lechner","role":"aut","display":"Lechner, Vera"},{"role":"aut","display":"Villiou, Maria","given":"Maria","family":"Villiou"},{"display":"Eis, Larissa","role":"aut","family":"Eis","given":"Larissa"},{"role":"aut","display":"Klein, Steffen","given":"Steffen","family":"Klein"},{"display":"Funaya, Charlotta","role":"aut","family":"Funaya","given":"Charlotta"},{"given":"Elisabetta A.","family":"Cavalcanti-Adam","role":"aut","display":"Cavalcanti-Adam, Elisabetta A."},{"family":"Graw","given":"Frederik","display":"Graw, Frederik","role":"aut"},{"display":"Selhuber-Unkel, Christine","role":"aut","family":"Selhuber-Unkel","given":"Christine"},{"display":"Rohr, Karl","role":"aut","family":"Rohr","given":"Karl"},{"role":"aut","display":"Chlanda, Petr","given":"Petr","family":"Chlanda"}],"language":["eng"],"relHost":[{"titleAlt":[{"title":"The EMBO journal online"}],"recId":"266022529","origin":[{"publisherPlace":"[London] ; Heidelberg ; Oxford [u.a.] ; London [u.a.] ; Hoboken, NJ [u.a.]","dateIssuedKey":"1982","dateIssuedDisp":"1982-","publisher":"Nature Publishing Group UK ; EMBO Press ; Oxford Univ. Press ; Nature Publ. Group ; Wiley"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"id":{"zdb":["1467419-1"],"issn":["1460-2075"],"doi":["10.1002/(ISSN)1460-2075"],"eki":["266022529"]},"pubHistory":["1.1982 -"],"part":{"pages":"3959-3982","issue":"14","text":"44(2025), 14 vom: Juli, Seite 3959-3982","volume":"44","year":"2025","extent":"24"},"name":{"displayForm":["publ. for the European Molecular Biology Organization"]},"note":["Gesehen am 18.02.2016","Fortsetzung der Druckausgabe"],"disp":"European Molecular Biology OrganizationThe EMBO journal","corporate":[{"role":"aut","display":"European Molecular Biology Organization"}],"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"EMBO journal","title":"The EMBO journal"}]}],"title":[{"title":"Morphology-dependent entry kinetics and spread of influenza A virus","title_sort":"Morphology-dependent entry kinetics and spread of influenza A virus"}],"id":{"eki":["1939454344"],"doi":["10.1038/s44318-025-00481-6"]},"type":{"bibl":"article-journal","media":"Online-Ressource"},"physDesc":[{"extent":"24 S.","noteIll":"Illustrationen, Diagramme"}],"note":["Online veröffentlicht: 9 June 2025","Gesehen am 27.10.2025"],"recId":"1939454344","name":{"displayForm":["Sarah Peterl, Carmen M. Lahr, Carl N. Schneider, Janis Meyer, Xenia Podlipensky, Vera Lechner, Maria Villiou, Larissa Eis, Steffen Klein, Charlotta Funaya, Elisabetta Ada Cavalcanti-Adam, Frederik Graw, Christine Selhuber-Unkel, Karl Rohr & Petr Chlanda"]},"origin":[{"dateIssuedKey":"2025","dateIssuedDisp":"Jul 2025"}]} | ||
| SRT | |a PETERLSARAMORPHOLOGY2025 | ||