Ultra-fast liquid chromatography detection of β-N-methylamine-l-alanine and its isomers in cycad seeds and cyanobacterial symbionts for neurotoxic risk assessment
The cyanobacterial neurotoxin has been implicated in various neurological disorders, posing a potential global health risk. Initial studies revealed alarming levels of β-N-methylamine-l-alanine (BMAA) in cyanobacteria, particularly in symbiotic species, suggesting widespread exposure. This study aim...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
1 May 2025
|
| In: |
Food and chemical toxicology
Year: 2025, Jahrgang: 202, Pages: 1-9 |
| ISSN: | 1873-6351 |
| DOI: | 10.1016/j.fct.2025.115503 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1016/j.fct.2025.115503 Verlag, kostenfrei, Volltext: https://www.sciencedirect.com/science/article/pii/S0278691525002716 
|
| Verfasserangaben: | Chandrabose Uthra, Sumathy Shunmugam, Karuppiah Nagaraj, Gangatharan Muralitharan, Mohammed F. Albeshr, Gunasekaran Velmurugan |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 193966859X | ||
| 003 | DE-627 | ||
| 005 | 20251120193656.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 251029s2025 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.fct.2025.115503 |2 doi | |
| 035 | |a (DE-627)193966859X | ||
| 035 | |a (DE-599)KXP193966859X | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 30 |2 sdnb | ||
| 100 | 1 | |a Uthra, Chandrabose |e VerfasserIn |0 (DE-588)1379668964 |0 (DE-627)1939332311 |4 aut | |
| 245 | 1 | 0 | |a Ultra-fast liquid chromatography detection of β-N-methylamine-l-alanine and its isomers in cycad seeds and cyanobacterial symbionts for neurotoxic risk assessment |c Chandrabose Uthra, Sumathy Shunmugam, Karuppiah Nagaraj, Gangatharan Muralitharan, Mohammed F. Albeshr, Gunasekaran Velmurugan |
| 246 | 3 | 3 | |a Ultra-fast liquid chromatography detection of beta-N-methylamine-l-alanine and its isomers in cycad seeds and cyanobacterial symbionts for neurotoxic risk assessment |
| 264 | 1 | |c 1 May 2025 | |
| 300 | |b Illustrationen | ||
| 300 | |a 9 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 29.10.2025 | ||
| 520 | |a The cyanobacterial neurotoxin has been implicated in various neurological disorders, posing a potential global health risk. Initial studies revealed alarming levels of β-N-methylamine-l-alanine (BMAA) in cyanobacteria, particularly in symbiotic species, suggesting widespread exposure. This study aimed to validate the efficacy of ultra-fast liquid chromatography (UFLC) technique for the detection and quantification of BMAA in various samples. Derivatizing agents, including 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) and N-(2-aminoethyl) glycine (AEG), were synthesized and confirmed via nuclear magnetic resonance (NMR) spectroscopy to enhance the detection of isomeric neurotoxic compounds. Among the samples analyzed cycad seeds, leaves, male cones, cyanobacterial symbionts, coralloid roots, and processed cycad seed flour BMAA and its isomers (2,4-diaminobutyric acid (2,4-DAB) and AEG) were detected in cycad seeds, cyanobacterial symbionts, and coralloid roots. The retention times for L-BMAA, AEG, and 2,4-DAB were 5.4, 5.6, and 6.1 min, respectively. Quantification revealed lower levels of these toxic isomers in seeds compared to high levels in cyanobacterial symbionts. Furthermore, UFLC methods effectively reduced the levels of neurotoxic compounds in cycad seeds to below detectable limits (6 × 103 ng/mL). This study underscores the utility of UFLC method combined with derivatization for the efficient detection and separation of L-BMAA and its isomers, providing a reliable approach for neurotoxin analysis. | ||
| 650 | 4 | |a Cyanobacterial neurotoxin | |
| 650 | 4 | |a Cyanobacterial symbionts | |
| 650 | 4 | |a Cycad seeds | |
| 650 | 4 | |a Neurotoxin quantification | |
| 650 | 4 | |a UFLC derivatization | |
| 650 | 4 | |a β-N-Methylamine;alanine (BMAA) | |
| 700 | 1 | |a Shunmugam, Sumathy |e VerfasserIn |4 aut | |
| 700 | 1 | |a Nagaraj, Karuppiah |e VerfasserIn |4 aut | |
| 700 | 1 | |a Muralitharan, Gangatharan |e VerfasserIn |4 aut | |
| 700 | 1 | |a Albeshr, Mohammed F. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Velmurugan, Gunasekaran |e VerfasserIn |0 (DE-588)1257460250 |0 (DE-627)1801727740 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Food and chemical toxicology |d New York, NY [u.a.] : Elsevier, 1982 |g 202(2025) vom: Aug., Artikel-ID 115503, Seite 1-9 |h Online-Ressource |w (DE-627)300898487 |w (DE-600)1483645-2 |w (DE-576)259270849 |x 1873-6351 |7 nnas |a Ultra-fast liquid chromatography detection of β-N-methylamine-l-alanine and its isomers in cycad seeds and cyanobacterial symbionts for neurotoxic risk assessment |
| 773 | 1 | 8 | |g volume:202 |g year:2025 |g month:08 |g elocationid:115503 |g pages:1-9 |g extent:9 |a Ultra-fast liquid chromatography detection of β-N-methylamine-l-alanine and its isomers in cycad seeds and cyanobacterial symbionts for neurotoxic risk assessment |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.fct.2025.115503 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S0278691525002716 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20251029 | ||
| 993 | |a Article | ||
| 994 | |a 2025 | ||
| 998 | |g 1257460250 |a Velmurugan, Gunasekaran |m 1257460250:Velmurugan, Gunasekaran |d 120000 |d 120200 |e 120000PV1257460250 |e 120200PV1257460250 |k 0/120000/ |k 1/120000/120200/ |p 6 |y j | ||
| 999 | |a KXP-PPN193966859X |e 4794160909 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"name":{"displayForm":["Chandrabose Uthra, Sumathy Shunmugam, Karuppiah Nagaraj, Gangatharan Muralitharan, Mohammed F. Albeshr, Gunasekaran Velmurugan"]},"note":["Gesehen am 29.10.2025"],"recId":"193966859X","person":[{"role":"aut","family":"Uthra","display":"Uthra, Chandrabose","given":"Chandrabose"},{"display":"Shunmugam, Sumathy","family":"Shunmugam","given":"Sumathy","role":"aut"},{"role":"aut","family":"Nagaraj","display":"Nagaraj, Karuppiah","given":"Karuppiah"},{"role":"aut","display":"Muralitharan, Gangatharan","family":"Muralitharan","given":"Gangatharan"},{"given":"Mohammed F.","display":"Albeshr, Mohammed F.","family":"Albeshr","role":"aut"},{"role":"aut","display":"Velmurugan, Gunasekaran","family":"Velmurugan","given":"Gunasekaran"}],"titleAlt":[{"title":"Ultra-fast liquid chromatography detection of beta-N-methylamine-l-alanine and its isomers in cycad seeds and cyanobacterial symbionts for neurotoxic risk assessment"}],"physDesc":[{"extent":"9 S.","noteIll":"Illustrationen"}],"title":[{"title_sort":"Ultra-fast liquid chromatography detection of β-N-methylamine-l-alanine and its isomers in cycad seeds and cyanobacterial symbionts for neurotoxic risk assessment","title":"Ultra-fast liquid chromatography detection of β-N-methylamine-l-alanine and its isomers in cycad seeds and cyanobacterial symbionts for neurotoxic risk assessment"}],"origin":[{"dateIssuedDisp":"1 May 2025","dateIssuedKey":"2025"}],"relHost":[{"note":["Gesehen am 29.03.11"],"pubHistory":["20.1982 - 50.2012; Vol. 51.2013 -"],"recId":"300898487","disp":"Ultra-fast liquid chromatography detection of β-N-methylamine-l-alanine and its isomers in cycad seeds and cyanobacterial symbionts for neurotoxic risk assessmentFood and chemical toxicology","physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"Food and chemical toxicology","title":"Food and chemical toxicology"}],"part":{"pages":"1-9","text":"202(2025) vom: Aug., Artikel-ID 115503, Seite 1-9","year":"2025","extent":"9","volume":"202"},"id":{"eki":["300898487"],"zdb":["1483645-2"],"issn":["1873-6351"]},"language":["eng"],"type":{"bibl":"periodical","media":"Online-Ressource"},"origin":[{"publisherPlace":"New York, NY [u.a.]","dateIssuedDisp":"1982-","dateIssuedKey":"1982","publisher":"Elsevier"}]}],"language":["eng"],"id":{"eki":["193966859X"],"doi":["10.1016/j.fct.2025.115503"]},"type":{"bibl":"article-journal","media":"Online-Ressource"}} | ||
| SRT | |a UTHRACHANDULTRAFASTL1202 | ||