Establishing an auxin-inducible GFP nanobody-based acute protein knockdown system to mimic hypomorphic mutations during early medaka embryogenesis

Creating hypomorphic mutations is crucial to study gene function in vivo, especially when null mutations result in (embryonic) lethality. This applies to enzymes involved in glycosylation that, when mutated in human patients, cause the disease congenital disorders of glycosylation (CDG). In order to...

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Main Authors: Pakari, Kaisa (Author) , Jakab, Sevinç (Author) , Rasch, Johanna (Author) , Sánchez Salvador, Encarnación (Author) , Wittbrodt, Beate (Author) , Thiel, Christian (Author) , Wittbrodt, Joachim (Author) , Thumberger, Thomas (Author)
Format: Article (Journal)
Language:English
Published: November 2025
In: Biology open
Year: 2025, Volume: 14, Issue: 11, Pages: 1-9
ISSN:2046-6390
DOI:10.1242/bio.062081
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1242/bio.062081
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Author Notes:Kaisa Pakari, Sevinç Jakab, Johanna Rasch, Encarnación Sánchez Salvador, Beate Wittbrodt, Christian Thiel, Joachim Wittbrodt, Thomas Thumberger
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Establishing an auxin-inducible GFP nanobody-based acute protein knockdown system to mimic hypomorphic mutations during early medaka embryogenesis by Pakari, Kaisa (Author) , Jakab, Sevinç (Author) , Sánchez Salvador, Encarnación (Author) , Thiel, Christian (Author) , Wittbrodt, Joachim (Author) , Thumberger, Thomas (Author) ,


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