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Cardioplegic machine perfusion of hearts donated after circulatory death

We compared the effects of ex-vivo machine perfusion (EVMP) of hearts donated after circulatory death (DCD) with the single-shot solutions HTK-N and Del Nido cardioplegia (DNC) on left-ventricular (LV) contractility and myocardial microcirculation. In a DCD pig model, hearts were maintained by EVMP...

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Hauptverfasser: Saemann, Lars (VerfasserIn) , Wachter, Kristina (VerfasserIn) , Großkopf, Anne (VerfasserIn) , Pohl, Sabine (VerfasserIn) , Georgevici, Adrian-Iustin (VerfasserIn) , Hoorn, Fabio (VerfasserIn) , Korkmaz-İçöz, Sevil (VerfasserIn) , Karck, Matthias (VerfasserIn) , Simm, Andreas (VerfasserIn) , Szabó, Gábor (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 08 September 2025
In: Journal of cardiovascular translational research
Year: 2025, Jahrgang: 18, Heft: 5, Pages: 1192-1203
ISSN:1937-5395
DOI:10.1007/s12265-025-10694-z
Online-Zugang:Resolving-System, kostenfrei: https://doi.org/10.1007/s12265-025-10694-z
Verlag, kostenfrei, Volltext: https://doi.org/10.1007/s12265-025-10694-z
Volltext
Verfasserangaben:Lars Saemann, Kristin Wächter, Anne Großkopf, Sabine Pohl, Adrian-Iustin Georgevici, Fabio Hoorn, Sevil Korkmaz-Icöz, Matthias Karck, Andreas Simm, Gábor Szabó
Beschreibung
Zusammenfassung:We compared the effects of ex-vivo machine perfusion (EVMP) of hearts donated after circulatory death (DCD) with the single-shot solutions HTK-N and Del Nido cardioplegia (DNC) on left-ventricular (LV) contractility and myocardial microcirculation. In a DCD pig model, hearts were maintained by EVMP with hypothermic, oxygenated HTK-N (DCD-HTK-N; N = 8) or DNC (DCD-DNC; N = 8) followed by reperfusion with blood, including assessment of contractility and microcirculation with Laser-Doppler-Flow (LDF). We performed transcriptomics using microarrays. In DCD-HTK-N, the ESP, dp/dtmax and dp/dtmin were significantly higher (p < 0.05) compared to DCD-DNC. Relative LDF was higher in DCD-HTK-N vs. DCD-DNC. Pathways related to inflammatory mediators, cAMP, ion channels, intracellular signaling, and cell death were regulated differently. In DCD-HTK-N, longevity-associated pathways were up-, and ageing-associated pathways were downregulated. EVMP of DCD hearts with HTK-N results in a superior LV function, microcirculation, and regulation of pathways with short- and long-term relevance compared to DNC.
Beschreibung:Gesehen am 16.01.2026
Beschreibung:Online Resource
ISSN:1937-5395
DOI:10.1007/s12265-025-10694-z

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