An approach to evaluate the effect of inflammatory microvesicles on Ca2+ handling in human-induced pluripotent stem cell-derived cardiomyocytes
Microvesicles (MV) isolated from septic individuals were observed to impact systemic hemodynamics and cardiac function. The aim of this in vitro study was to analyze the effects of TNFα-induced endothelial MV (TMV) and MV from septic patients (SMV) on beating frequency and Ca2+ transient kinetics of...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
28 August 2025
|
| In: |
Experimental biology and medicine
Year: 2025, Jahrgang: 250, Pages: 1-14 |
| ISSN: | 1535-3699 |
| DOI: | 10.3389/ebm.2025.10461 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.3389/ebm.2025.10461 Verlag, kostenfrei, Volltext: https://www.ebm-journal.org/journals/experimental-biology-and-medicine/articles/10.3389/ebm.2025.10461/full 
|
| Verfasserangaben: | Dania Fischer, Mishkaat Sha’sha’a, Judith Schenz, Aycan Tayan, Christina Mertens, Sebastian O. Decker, Nadia Gallenstein, Maximilian Dietrich, Trim Lajqi, Anna Hafner, Markus A. Weigand and Nina D. Ullrich |
MARC
| LEADER | 00000naa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1950286851 | ||
| 003 | DE-627 | ||
| 005 | 20260127094806.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 260127s2025 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3389/ebm.2025.10461 |2 doi | |
| 035 | |a (DE-627)1950286851 | ||
| 035 | |a (DE-599)KXP1950286851 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Fischer, Dania |e VerfasserIn |0 (DE-588)122172651X |0 (DE-627)173911311X |4 aut | |
| 245 | 1 | 3 | |a An approach to evaluate the effect of inflammatory microvesicles on Ca2+ handling in human-induced pluripotent stem cell-derived cardiomyocytes |c Dania Fischer, Mishkaat Sha’sha’a, Judith Schenz, Aycan Tayan, Christina Mertens, Sebastian O. Decker, Nadia Gallenstein, Maximilian Dietrich, Trim Lajqi, Anna Hafner, Markus A. Weigand and Nina D. Ullrich |
| 246 | 3 | 3 | |a An approach to evaluate the effect of inflammatory microvesicles on Ca 2 + handling in human-induced pluripotent stem cell-derived cardiomyocytes |
| 246 | 3 | 3 | |a An approach to evaluate the effect of inflammatory microvesicles on Ca 2+ handling in human-induced pluripotent stem cell-derived cardiomyocytes |
| 264 | 1 | |c 28 August 2025 | |
| 300 | |b Illustrationen | ||
| 300 | |a 14 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Im Titel ist "2" und "+" jeweils hochgestellt | ||
| 500 | |a Gesehen am 27.01.2026 | ||
| 520 | |a Microvesicles (MV) isolated from septic individuals were observed to impact systemic hemodynamics and cardiac function. The aim of this in vitro study was to analyze the effects of TNFα-induced endothelial MV (TMV) and MV from septic patients (SMV) on beating frequency and Ca2+ transient kinetics of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM). MV were isolated from supernatants of TNFα-stimulated primary human pulmonary microvascular endothelial cells (HPMEC) and plasma from 20 sepsis patients by ultracentrifugation and quantified using flow cytometry. Spontaneous Ca2+ transients were measured in hiPSC-CM using the Ca2+-sensitive ratiometric indicator fura-2 at different time points of incubation with different MV concentrations. At 16 h of incubation, higher MV concentrations showed significant differences, especially regarding decay and beating frequency. Despite high variability, at 10 × 106 MV/mL and 16 h of incubation, TMV significantly decreased frequency compared to control MV (CMV). SMV from septic patients did not reveal any significant effects on Ca2+ transients under these experimental settings. MV isolated from control or TNFα-treated HPMEC affected Ca2+ handling and spontaneous activity of hiPSC-CM, however, the measured effects were not consistent throughout the different conditions. Further refinement of the experiment conditions is needed to specify the exact conditions for crosstalk between endothelium-derived MV and cardiomyocytes. | ||
| 700 | 1 | |a Sha’sha’a, Mishkaat |e VerfasserIn |0 (DE-588)1388066335 |0 (DE-627)1950291901 |4 aut | |
| 700 | 1 | |a Schenz, Judith |e VerfasserIn |0 (DE-588)1163038768 |0 (DE-627)1027280935 |0 (DE-576)507754875 |4 aut | |
| 700 | 1 | |a Tayan, Aycan |e VerfasserIn |0 (DE-588)1366302370 |0 (DE-627)1926227506 |4 aut | |
| 700 | 1 | |a Mertens, Christina |e VerfasserIn |0 (DE-588)1160734623 |0 (DE-627)1024158659 |0 (DE-576)506156974 |4 aut | |
| 700 | 1 | |a Decker, Sebastian |d 1984- |e VerfasserIn |0 (DE-588)139936041 |0 (DE-627)703511785 |0 (DE-576)313600880 |4 aut | |
| 700 | 1 | |a Gallenstein, Nadia |d 1991- |e VerfasserIn |0 (DE-588)1284314057 |0 (DE-627)1840011734 |4 aut | |
| 700 | 1 | |a Dietrich, Maximilian |d 1991- |e VerfasserIn |0 (DE-588)1172010889 |0 (DE-627)1040838707 |0 (DE-576)513949259 |4 aut | |
| 700 | 1 | |a Lajqi, Trim |d 1990- |e VerfasserIn |0 (DE-588)1184939764 |0 (DE-627)1663824916 |4 aut | |
| 700 | 1 | |a Hafner, Anna |d 1988- |e VerfasserIn |0 (DE-588)1185195971 |0 (DE-627)1664548661 |4 aut | |
| 700 | 1 | |a Weigand, Markus A. |d 1967- |e VerfasserIn |0 (DE-588)1064970435 |0 (DE-627)815375239 |0 (DE-576)424658666 |4 aut | |
| 700 | 1 | |a Ullrich, Nina D. |e VerfasserIn |0 (DE-588)1130195619 |0 (DE-627)884562549 |0 (DE-576)486667251 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Experimental biology and medicine |d Lausanne : Frontiers, 2001 |g 250(2025), Artikel-ID 10461, Seite 1-14 |h Online-Ressource |w (DE-627)320606864 |w (DE-600)2020856-X |w (DE-576)091139228 |x 1535-3699 |7 nnas |a An approach to evaluate the effect of inflammatory microvesicles on Ca2+ handling in human-induced pluripotent stem cell-derived cardiomyocytes |
| 773 | 1 | 8 | |g volume:250 |g year:2025 |g elocationid:10461 |g pages:1-14 |g extent:14 |a An approach to evaluate the effect of inflammatory microvesicles on Ca2+ handling in human-induced pluripotent stem cell-derived cardiomyocytes |
| 856 | 4 | 0 | |u https://doi.org/10.3389/ebm.2025.10461 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |7 0 |
| 856 | 4 | 0 | |u https://www.ebm-journal.org/journals/experimental-biology-and-medicine/articles/10.3389/ebm.2025.10461/full |x Verlag |z kostenfrei |3 Volltext |7 0 |
| 951 | |a AR | ||
| 992 | |a 20260127 | ||
| 993 | |a Article | ||
| 994 | |a 2025 | ||
| 998 | |g 1130195619 |a Ullrich, Nina D. |m 1130195619:Ullrich, Nina D. |p 12 |y j | ||
| 998 | |g 1064970435 |a Weigand, Markus A. |m 1064970435:Weigand, Markus A. |d 910000 |d 910300 |e 910000PW1064970435 |e 910300PW1064970435 |k 0/910000/ |k 1/910000/910300/ |p 11 | ||
| 998 | |g 1185195971 |a Hafner, Anna |m 1185195971:Hafner, Anna |d 910000 |d 910300 |e 910000PH1185195971 |e 910300PH1185195971 |k 0/910000/ |k 1/910000/910300/ |p 10 | ||
| 998 | |g 1184939764 |a Lajqi, Trim |m 1184939764:Lajqi, Trim |d 910000 |d 910500 |d 50000 |e 910000PL1184939764 |e 910500PL1184939764 |e 50000PL1184939764 |k 0/910000/ |k 1/910000/910500/ |k 0/50000/ |p 9 | ||
| 998 | |g 1172010889 |a Dietrich, Maximilian |m 1172010889:Dietrich, Maximilian |d 910000 |d 910300 |d 50000 |e 910000PD1172010889 |e 910300PD1172010889 |e 50000PD1172010889 |k 0/910000/ |k 1/910000/910300/ |k 0/50000/ |p 8 | ||
| 998 | |g 1284314057 |a Gallenstein, Nadia |m 1284314057:Gallenstein, Nadia |d 50000 |d 53100 |e 50000PG1284314057 |e 53100PG1284314057 |k 0/50000/ |k 1/50000/53100/ |p 7 | ||
| 998 | |g 139936041 |a Decker, Sebastian |m 139936041:Decker, Sebastian |d 910000 |d 910300 |d 50000 |e 910000PD139936041 |e 910300PD139936041 |e 50000PD139936041 |k 0/910000/ |k 1/910000/910300/ |k 0/50000/ |p 6 | ||
| 998 | |g 1160734623 |a Mertens, Christina |m 1160734623:Mertens, Christina |d 910000 |d 910300 |e 910000PM1160734623 |e 910300PM1160734623 |k 0/910000/ |k 1/910000/910300/ |p 5 | ||
| 998 | |g 1366302370 |a Tayan, Aycan |m 1366302370:Tayan, Aycan |d 50000 |e 50000PT1366302370 |k 0/50000/ |p 4 | ||
| 998 | |g 1163038768 |a Schenz, Judith |m 1163038768:Schenz, Judith |d 910000 |d 910300 |e 910000PS1163038768 |e 910300PS1163038768 |k 0/910000/ |k 1/910000/910300/ |p 3 | ||
| 998 | |g 1388066335 |a Sha’sha’a, Mishkaat |m 1388066335:Sha’sha’a, Mishkaat |d 50000 |e 50000PS1388066335 |k 0/50000/ |p 2 | ||
| 998 | |g 122172651X |a Fischer, Dania |m 122172651X:Fischer, Dania |d 910000 |d 910300 |d 50000 |e 910000PF122172651X |e 910300PF122172651X |e 50000PF122172651X |k 0/910000/ |k 1/910000/910300/ |k 0/50000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1950286851 |e 4861005051 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"id":{"doi":["10.3389/ebm.2025.10461"],"eki":["1950286851"]},"origin":[{"dateIssuedKey":"2025","dateIssuedDisp":"28 August 2025"}],"relHost":[{"note":["Gesehen am 25.03.2024","Fortsetzung der Druck-Ausgabe"],"physDesc":[{"extent":"Online-Ressource"}],"language":["eng"],"title":[{"title_sort":"Experimental biology and medicine","title":"Experimental biology and medicine","subtitle":"EBM ; official journal of the Society for Experimental Biology and Medicine"}],"origin":[{"publisher":"Frontiers ; Blackwell Science ; HighWire Press ; Sage","dateIssuedKey":"2001","dateIssuedDisp":"2001-","publisherPlace":"Lausanne ; Oxford ; Stanford, Calif. ; London"}],"id":{"zdb":["2020856-X"],"eki":["320606864"],"issn":["1535-3699"]},"part":{"year":"2025","extent":"14","text":"250(2025), Artikel-ID 10461, Seite 1-14","pages":"1-14","volume":"250"},"disp":"An approach to evaluate the effect of inflammatory microvesicles on Ca2+ handling in human-induced pluripotent stem cell-derived cardiomyocytesExperimental biology and medicine","pubHistory":["Volume 226, issue 1 (Jan. 2001)-"],"titleAlt":[{"title":"PSEBM Online"},{"title":"EBM"},{"title":"Proceedings of the Society for Experimental Biology and Medicine"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"recId":"320606864"}],"name":{"displayForm":["Dania Fischer, Mishkaat Sha’sha’a, Judith Schenz, Aycan Tayan, Christina Mertens, Sebastian O. Decker, Nadia Gallenstein, Maximilian Dietrich, Trim Lajqi, Anna Hafner, Markus A. Weigand and Nina D. Ullrich"]},"note":["Im Titel ist \"2\" und \"+\" jeweils hochgestellt","Gesehen am 27.01.2026"],"title":[{"title":"An approach to evaluate the effect of inflammatory microvesicles on Ca2+ handling in human-induced pluripotent stem cell-derived cardiomyocytes","title_sort":"approach to evaluate the effect of inflammatory microvesicles on Ca2+ handling in human-induced pluripotent stem cell-derived cardiomyocytes"}],"language":["eng"],"physDesc":[{"noteIll":"Illustrationen","extent":"14 S."}],"recId":"1950286851","type":{"media":"Online-Ressource","bibl":"article-journal"},"titleAlt":[{"title":"An approach to evaluate the effect of inflammatory microvesicles on Ca 2 + handling in human-induced pluripotent stem cell-derived cardiomyocytes"},{"title":"An approach to evaluate the effect of inflammatory microvesicles on Ca 2+ handling in human-induced pluripotent stem cell-derived cardiomyocytes"}],"person":[{"display":"Fischer, Dania","family":"Fischer","role":"aut","given":"Dania"},{"given":"Mishkaat","display":"Sha’sha’a, Mishkaat","family":"Sha’sha’a","role":"aut"},{"given":"Judith","display":"Schenz, Judith","family":"Schenz","role":"aut"},{"given":"Aycan","role":"aut","family":"Tayan","display":"Tayan, Aycan"},{"given":"Christina","display":"Mertens, Christina","family":"Mertens","role":"aut"},{"given":"Sebastian","role":"aut","display":"Decker, Sebastian","family":"Decker"},{"display":"Gallenstein, Nadia","family":"Gallenstein","role":"aut","given":"Nadia"},{"role":"aut","family":"Dietrich","display":"Dietrich, Maximilian","given":"Maximilian"},{"given":"Trim","role":"aut","display":"Lajqi, Trim","family":"Lajqi"},{"role":"aut","display":"Hafner, Anna","family":"Hafner","given":"Anna"},{"given":"Markus A.","role":"aut","display":"Weigand, Markus A.","family":"Weigand"},{"role":"aut","display":"Ullrich, Nina D.","family":"Ullrich","given":"Nina D."}]} | ||
| SRT | |a FISCHERDANAPPROACHTO2820 | ||