New cell lines expanding the diversity of Ewing sarcoma models
Ewing sarcoma (EwS) is a highly aggressive, malignant, solid tumor of childhood, and adolescence. Most EwS develop in bone, while it originates from connective, adipose, or muscle tissue less frequently. We report the establishment of new human EwS cell lines, all of which carry a t(11;22)(q24;q12)...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
26 September 2025
|
| In: |
International journal of cancer
Year: 2026, Volume: 158, Issue: 3, Pages: 642-655 |
| ISSN: | 1097-0215 |
| DOI: | 10.1002/ijc.70172 |
| Online Access: | Verlag, kostenfrei, Volltext: https://doi.org/10.1002/ijc.70172 Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/ijc.70172 
|
| Author Notes: | Maximilian Kerkhoff, Christiane Schaefer, Wilhelm G. Dirks, Dawid Krzeciesa, Maximilian Bretschneider, Pauline R. Plaumann, Wiebke K. Guder, Arne Streitbürger, Sonja Herter, Heike Peterziel, Ina Oehme, Felina Zahnow, Thomas G.P. Grünewald, Uta Dirksen |
MARC
| LEADER | 00000naa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1950995720 | ||
| 003 | DE-627 | ||
| 005 | 20260203115017.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 260203s2026 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/ijc.70172 |2 doi | |
| 035 | |a (DE-627)1950995720 | ||
| 035 | |a (DE-599)KXP1950995720 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Kerkhoff, Maximilian |e VerfasserIn |0 (DE-588)1337891878 |0 (DE-627)1897718764 |4 aut | |
| 245 | 1 | 0 | |a New cell lines expanding the diversity of Ewing sarcoma models |c Maximilian Kerkhoff, Christiane Schaefer, Wilhelm G. Dirks, Dawid Krzeciesa, Maximilian Bretschneider, Pauline R. Plaumann, Wiebke K. Guder, Arne Streitbürger, Sonja Herter, Heike Peterziel, Ina Oehme, Felina Zahnow, Thomas G.P. Grünewald, Uta Dirksen |
| 264 | 1 | |c 26 September 2025 | |
| 300 | |b Illustrationen | ||
| 300 | |a 14 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 03.02.2026 | ||
| 520 | |a Ewing sarcoma (EwS) is a highly aggressive, malignant, solid tumor of childhood, and adolescence. Most EwS develop in bone, while it originates from connective, adipose, or muscle tissue less frequently. We report the establishment of new human EwS cell lines, all of which carry a t(11;22)(q24;q12) translocation generating the oncogenic transcription factor EWSR1::FLI1. Sequencing of the chimeric mRNAs indicated genomic DNA breakpoints localized in intron 8 of the EWSR1 gene and three different introns of the translocation partner gene FLI1. While three EwS cell lines carry the EWSR1::FLI1 fusions of ex7/ex6 (type I) or ex7/ex5 (type II), the EWSR1::FLI1 fusion variant ex7/ex7 (type IV) is described for the first time in a continuous EwS model. The cell lines presented genomic, epigenomic, and transcriptomic stability over a period of 6 months, though some variations in the chromosomal aberrations were observed in one cell line. TP53, STAG2, and CDKN2A/B mutations were the most frequent and most relevant mutations in our cell line panel. The TP53 mutational status seemed to have the biggest impact on drug sensitivity profiles. The new EwS models presented here may help to identify small molecule inhibitors that act directly on EWSR1::FLI1 fusion proteins or uncover other genetic vulnerabilities of the altered epigenome and transcriptome in EwS, which would contribute to a better understanding of Ewing sarcoma tumorigenesis. | ||
| 650 | 4 | |a (epi-)genomics | |
| 650 | 4 | |a cell line establishment | |
| 650 | 4 | |a drug screen | |
| 650 | 4 | |a Ewing sarcoma | |
| 650 | 4 | |a transcriptomics | |
| 700 | 1 | |a Schaefer, Christiane |e VerfasserIn |4 aut | |
| 700 | 1 | |a Dirks, Wilhelm G. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Krzeciesa, Dawid |e VerfasserIn |4 aut | |
| 700 | 1 | |a Bretschneider, Maximilian |e VerfasserIn |4 aut | |
| 700 | 1 | |a Plaumann, Pauline R. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Guder, Wiebke K. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Streitbürger, Arne |e VerfasserIn |4 aut | |
| 700 | 1 | |a Herter, Sonja |d 1996- |e VerfasserIn |0 (DE-588)1254275428 |0 (DE-627)1796888303 |4 aut | |
| 700 | 1 | |a Peterziel, Heike |e VerfasserIn |0 (DE-588)1079032606 |0 (DE-627)840098022 |0 (DE-576)452152348 |4 aut | |
| 700 | 1 | |a Oehme, Ina |d 1974- |e VerfasserIn |0 (DE-588)129573388 |0 (DE-627)473352621 |0 (DE-576)297728652 |4 aut | |
| 700 | 1 | |a Zahnow, Felina |e VerfasserIn |4 aut | |
| 700 | 1 | |a Grünewald, Thomas G. P. |d 1980- |e VerfasserIn |0 (DE-588)13673426X |0 (DE-627)585870365 |0 (DE-576)301246432 |4 aut | |
| 700 | 1 | |a Dirksen, Uta |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International journal of cancer |d Bognor Regis : Wiley-Liss, 1966 |g 158(2026), 3, Seite 642-655 |h Online-Ressource |w (DE-627)269532781 |w (DE-600)1474822-8 |w (DE-576)079876129 |x 1097-0215 |7 nnas |a New cell lines expanding the diversity of Ewing sarcoma models |
| 773 | 1 | 8 | |g volume:158 |g year:2026 |g number:3 |g pages:642-655 |g extent:14 |a New cell lines expanding the diversity of Ewing sarcoma models |
| 856 | 4 | 0 | |u https://doi.org/10.1002/ijc.70172 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |7 0 |
| 856 | 4 | 0 | |u https://onlinelibrary.wiley.com/doi/abs/10.1002/ijc.70172 |x Verlag |z kostenfrei |3 Volltext |7 0 |
| 951 | |a AR | ||
| 992 | |a 20260203 | ||
| 993 | |a Article | ||
| 994 | |a 2026 | ||
| 998 | |g 13673426X |a Grünewald, Thomas G. P. |m 13673426X:Grünewald, Thomas G. P. |d 910000 |d 912000 |e 910000PG13673426X |e 912000PG13673426X |k 0/910000/ |k 1/910000/912000/ |p 13 | ||
| 998 | |g 129573388 |a Oehme, Ina |m 129573388:Oehme, Ina |d 50000 |d 910000 |d 910500 |e 50000PO129573388 |e 910000PO129573388 |e 910500PO129573388 |k 0/50000/ |k 0/910000/ |k 1/910000/910500/ |p 11 | ||
| 998 | |g 1079032606 |a Peterziel, Heike |m 1079032606:Peterziel, Heike |d 50000 |e 50000PP1079032606 |k 0/50000/ |p 10 | ||
| 999 | |a KXP-PPN1950995720 |e 4874820522 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"name":{"displayForm":["Maximilian Kerkhoff, Christiane Schaefer, Wilhelm G. Dirks, Dawid Krzeciesa, Maximilian Bretschneider, Pauline R. Plaumann, Wiebke K. Guder, Arne Streitbürger, Sonja Herter, Heike Peterziel, Ina Oehme, Felina Zahnow, Thomas G.P. Grünewald, Uta Dirksen"]},"language":["eng"],"note":["Gesehen am 03.02.2026"],"recId":"1950995720","person":[{"given":"Maximilian","display":"Kerkhoff, Maximilian","role":"aut","family":"Kerkhoff"},{"given":"Christiane","display":"Schaefer, Christiane","role":"aut","family":"Schaefer"},{"family":"Dirks","role":"aut","display":"Dirks, Wilhelm G.","given":"Wilhelm G."},{"role":"aut","family":"Krzeciesa","given":"Dawid","display":"Krzeciesa, Dawid"},{"family":"Bretschneider","role":"aut","given":"Maximilian","display":"Bretschneider, Maximilian"},{"family":"Plaumann","role":"aut","given":"Pauline R.","display":"Plaumann, Pauline R."},{"display":"Guder, Wiebke K.","given":"Wiebke K.","role":"aut","family":"Guder"},{"role":"aut","family":"Streitbürger","display":"Streitbürger, Arne","given":"Arne"},{"given":"Sonja","display":"Herter, Sonja","role":"aut","family":"Herter"},{"given":"Heike","display":"Peterziel, Heike","role":"aut","family":"Peterziel"},{"role":"aut","family":"Oehme","given":"Ina","display":"Oehme, Ina"},{"role":"aut","family":"Zahnow","display":"Zahnow, Felina","given":"Felina"},{"family":"Grünewald","role":"aut","display":"Grünewald, Thomas G. P.","given":"Thomas G. P."},{"given":"Uta","display":"Dirksen, Uta","role":"aut","family":"Dirksen"}],"physDesc":[{"noteIll":"Illustrationen","extent":"14 S."}],"id":{"doi":["10.1002/ijc.70172"],"eki":["1950995720"]},"type":{"media":"Online-Ressource","bibl":"article-journal"},"title":[{"title_sort":"New cell lines expanding the diversity of Ewing sarcoma models","title":"New cell lines expanding the diversity of Ewing sarcoma models"}],"origin":[{"dateIssuedDisp":"26 September 2025","dateIssuedKey":"2026"}],"relHost":[{"title":[{"title":"International journal of cancer","subtitle":"publication of the International Union against Cancer (UICC) = Journal international du cancer","title_sort":"International journal of cancer"}],"id":{"zdb":["1474822-8"],"doi":["10.1002/(ISSN)1097-0215"],"eki":["269532781"],"issn":["1097-0215"]},"physDesc":[{"extent":"Online-Ressource"}],"titleTranslated":[{"translated":"Journal international du cancer"}],"origin":[{"dateIssuedDisp":"1966-","publisher":"Wiley-Liss","publisherPlace":"Bognor Regis","dateIssuedKey":"1966"}],"recId":"269532781","corporate":[{"display":"International Union against Cancer","role":"isb"}],"disp":"New cell lines expanding the diversity of Ewing sarcoma modelsInternational journal of cancer","type":{"media":"Online-Ressource","bibl":"periodical"},"titleAlt":[{"title":"Predictive oncology"}],"language":["eng"],"part":{"volume":"158","year":"2026","text":"158(2026), 3, Seite 642-655","pages":"642-655","extent":"14","issue":"3"},"note":["Gesehen am 28.02.08","Darin: Predictive oncology"],"pubHistory":["1.1966 -"]}]} | ||
| SRT | |a KERKHOFFMANEWCELLLIN2620 | ||