Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cells
Mitosis is a critical phase of the cell cycle and a vulnerable point where cancer cells can be disrupted, causing cell death and inhibiting tumor growth. Challenges such as drug resistance persist in clinical applications. During mitosis, mRNA translation is generally downregulated, while non-canoni...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
27 August 2025
|
| In: |
Nature Communications
Year: 2025, Volume: 16, Pages: 1-15 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-025-63405-2 |
| Online Access: | Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41467-025-63405-2 Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41467-025-63405-2 
|
| Author Notes: | Alexander Kowar, Jonas P. Becker, Rossella Del Pizzo, Zhiwei Tang, Julien Champagne, Kathrin Wellach, Kiana Samimi, Ariel Galindo-Albarrán, Pierre-René Körner, Jasmine Montenegro Navarro, Andrés Elía, Fiona Megan Tilghman, Hanan Sakeer, Marco Antonio Mendoza-Parra, Angelika B. Riemer, Reuven Agami, and Fabricio Loayza-Puch |
MARC
| LEADER | 00000naa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1951021258 | ||
| 003 | DE-627 | ||
| 005 | 20260203153552.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 260203s2025 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s41467-025-63405-2 |2 doi | |
| 035 | |a (DE-627)1951021258 | ||
| 035 | |a (DE-599)KXP1951021258 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Kowar, Alexander |d 1990- |e VerfasserIn |0 (DE-588)1364149427 |0 (DE-627)192400254X |4 aut | |
| 245 | 1 | 0 | |a Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cells |c Alexander Kowar, Jonas P. Becker, Rossella Del Pizzo, Zhiwei Tang, Julien Champagne, Kathrin Wellach, Kiana Samimi, Ariel Galindo-Albarrán, Pierre-René Körner, Jasmine Montenegro Navarro, Andrés Elía, Fiona Megan Tilghman, Hanan Sakeer, Marco Antonio Mendoza-Parra, Angelika B. Riemer, Reuven Agami, and Fabricio Loayza-Puch |
| 264 | 1 | |c 27 August 2025 | |
| 300 | |b Illustrationen | ||
| 300 | |a 15 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 03.02.2026 | ||
| 520 | |a Mitosis is a critical phase of the cell cycle and a vulnerable point where cancer cells can be disrupted, causing cell death and inhibiting tumor growth. Challenges such as drug resistance persist in clinical applications. During mitosis, mRNA translation is generally downregulated, while non-canonical translation of specific transcripts continues. Here, we show that mitotic cancer cells redistribute ribosomes toward the 5′ untranslated region (5′ UTR) and beginning of the coding sequence (CDS), enhancing translation of thousands of upstream open reading frames (uORFs) and upstream overlapping open reading frames (uoORFs). This mitotic induction of uORF/uoORF enriches human leukocyte antigen (HLA) presentation of non-canonical peptides on the surface of cancer cells after mitotic inhibitor treatment. Functional assays indicate these epitopes provoke cancer-cell killing by T cells. Our findings highlight the therapeutic potential of targeting uORF/uoORF-derived epitopes with mitotic inhibitors to enhance immune recognition and tumor cell elimination. | ||
| 650 | 4 | |a Cellular immunity | |
| 650 | 4 | |a Mitosis | |
| 650 | 4 | |a Ribosome | |
| 700 | 1 | |a Becker, Jonas Philipp |d 1991- |e VerfasserIn |0 (DE-588)1050230825 |0 (DE-627)783666160 |0 (DE-576)404441149 |4 aut | |
| 700 | 1 | |a Pizzo, Rossella del |e VerfasserIn |0 (DE-588)1364148803 |0 (DE-627)1924001446 |4 aut | |
| 700 | 1 | |a Tang, Zhiwei |e VerfasserIn |4 aut | |
| 700 | 1 | |a Champagne, Julien |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wellach, Kathrin |d 1995- |e VerfasserIn |0 (DE-588)1188440403 |0 (DE-627)1667391542 |4 aut | |
| 700 | 1 | |a Samimi, Kiana |e VerfasserIn |4 aut | |
| 700 | 1 | |a Galindo-Albarrán, Ariel |e VerfasserIn |4 aut | |
| 700 | 1 | |a Körner, Pierre-René |e VerfasserIn |4 aut | |
| 700 | 1 | |a Montenegro Navarro, Jasmine |e VerfasserIn |4 aut | |
| 700 | 1 | |a Elía, Andrés |e VerfasserIn |4 aut | |
| 700 | 1 | |a Tilghman, Fiona Megan |e VerfasserIn |4 aut | |
| 700 | 1 | |a Sakeer, Hanan |e VerfasserIn |4 aut | |
| 700 | 1 | |a Mendoza-Parra, Marco Antonio |e VerfasserIn |4 aut | |
| 700 | 1 | |a Riemer, Angelika |e VerfasserIn |0 (DE-588)1167191420 |0 (DE-627)1030878013 |0 (DE-576)511025688 |4 aut | |
| 700 | 1 | |a Agami, Reuven |e VerfasserIn |4 aut | |
| 700 | 1 | |a Loayza-Puch, Fabricio |e VerfasserIn |0 (DE-588)1266356436 |0 (DE-627)1815195916 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nature Communications |d [London] : Springer Nature, 2010 |g 16(2025), Artikel-ID 8008, Seite 1-15 |h Online-Ressource |w (DE-627)626457688 |w (DE-600)2553671-0 |w (DE-576)331555905 |x 2041-1723 |7 nnas |a Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cells |
| 773 | 1 | 8 | |g volume:16 |g year:2025 |g elocationid:8008 |g pages:1-15 |g extent:15 |a Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cells |
| 856 | 4 | 0 | |u https://doi.org/10.1038/s41467-025-63405-2 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |7 0 |
| 856 | 4 | 0 | |u https://www.nature.com/articles/s41467-025-63405-2 |x Verlag |z kostenfrei |3 Volltext |7 0 |
| 951 | |a AR | ||
| 992 | |a 20260203 | ||
| 993 | |a Article | ||
| 994 | |a 2025 | ||
| 998 | |g 1266356436 |a Loayza-Puch, Fabricio |m 1266356436:Loayza-Puch, Fabricio |d 50000 |e 50000PL1266356436 |k 0/50000/ |p 17 |y j | ||
| 998 | |g 1167191420 |a Riemer, Angelika |m 1167191420:Riemer, Angelika |d 50000 |e 50000PR1167191420 |k 0/50000/ |p 15 | ||
| 998 | |g 1188440403 |a Wellach, Kathrin |m 1188440403:Wellach, Kathrin |d 140000 |e 140000PW1188440403 |k 0/140000/ |p 6 | ||
| 998 | |g 1364148803 |a Pizzo, Rossella del |m 1364148803:Pizzo, Rossella del |d 50000 |e 50000PP1364148803 |k 0/50000/ |p 3 | ||
| 998 | |g 1364149427 |a Kowar, Alexander |m 1364149427:Kowar, Alexander |p 1 |x j | ||
| 999 | |a KXP-PPN1951021258 |e 4874982026 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"id":{"doi":["10.1038/s41467-025-63405-2"],"eki":["1951021258"]},"name":{"displayForm":["Alexander Kowar, Jonas P. Becker, Rossella Del Pizzo, Zhiwei Tang, Julien Champagne, Kathrin Wellach, Kiana Samimi, Ariel Galindo-Albarrán, Pierre-René Körner, Jasmine Montenegro Navarro, Andrés Elía, Fiona Megan Tilghman, Hanan Sakeer, Marco Antonio Mendoza-Parra, Angelika B. Riemer, Reuven Agami, and Fabricio Loayza-Puch"]},"physDesc":[{"noteIll":"Illustrationen","extent":"15 S."}],"recId":"1951021258","type":{"bibl":"article-journal","media":"Online-Ressource"},"language":["eng"],"note":["Gesehen am 03.02.2026"],"title":[{"title":"Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cells","title_sort":"Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cells"}],"origin":[{"dateIssuedKey":"2025","dateIssuedDisp":"27 August 2025"}],"person":[{"display":"Kowar, Alexander","given":"Alexander","role":"aut","family":"Kowar"},{"given":"Jonas Philipp","role":"aut","family":"Becker","display":"Becker, Jonas Philipp"},{"family":"Pizzo","role":"aut","given":"Rossella del","display":"Pizzo, Rossella del"},{"family":"Tang","given":"Zhiwei","role":"aut","display":"Tang, Zhiwei"},{"given":"Julien","role":"aut","family":"Champagne","display":"Champagne, Julien"},{"display":"Wellach, Kathrin","given":"Kathrin","role":"aut","family":"Wellach"},{"display":"Samimi, Kiana","family":"Samimi","role":"aut","given":"Kiana"},{"role":"aut","given":"Ariel","family":"Galindo-Albarrán","display":"Galindo-Albarrán, Ariel"},{"display":"Körner, Pierre-René","given":"Pierre-René","role":"aut","family":"Körner"},{"family":"Montenegro Navarro","given":"Jasmine","role":"aut","display":"Montenegro Navarro, Jasmine"},{"family":"Elía","given":"Andrés","role":"aut","display":"Elía, Andrés"},{"display":"Tilghman, Fiona Megan","role":"aut","given":"Fiona Megan","family":"Tilghman"},{"display":"Sakeer, Hanan","family":"Sakeer","given":"Hanan","role":"aut"},{"role":"aut","given":"Marco Antonio","family":"Mendoza-Parra","display":"Mendoza-Parra, Marco Antonio"},{"display":"Riemer, Angelika","given":"Angelika","role":"aut","family":"Riemer"},{"given":"Reuven","role":"aut","family":"Agami","display":"Agami, Reuven"},{"given":"Fabricio","role":"aut","family":"Loayza-Puch","display":"Loayza-Puch, Fabricio"}],"relHost":[{"note":["Gesehen am 13.06.24"],"language":["eng"],"type":{"bibl":"periodical","media":"Online-Ressource"},"part":{"pages":"1-15","year":"2025","text":"16(2025), Artikel-ID 8008, Seite 1-15","volume":"16","extent":"15"},"title":[{"title_sort":"Nature Communications","title":"Nature Communications"}],"id":{"issn":["2041-1723"],"eki":["626457688"],"zdb":["2553671-0"]},"pubHistory":["2010-"],"origin":[{"publisher":"Springer Nature ; Nature Publishing Group UK","publisherPlace":"[London] ; [London]","dateIssuedDisp":"[2010]-"}],"disp":"Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cellsNature Communications","physDesc":[{"extent":"Online-Ressource"}],"recId":"626457688"}]} | ||
| SRT | |a KOWARALEXAUPSTREAMOP2720 | ||