Multiplex 3D nanoscopy resolves nanoarchitecture of human immunodeficiency virus
Resolving the nanoscale organization of viral and host proteins is important to understanding virion assembly and infectivity. Here, we present a robust framework for multiplexed optical 3D super-resolution microscopy of human immunodeficiency virus type 1 (HIV-1) particles using minimal fluorescenc...
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| Hauptverfasser: | , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
20 January 2026
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| In: |
ACS nano
Year: 2026, Jahrgang: 20, Heft: 2, Pages: 1911-1927 |
| ISSN: | 1936-086X |
| DOI: | 10.1021/acsnano.5c12222 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/acsnano.5c12222
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| Verfasserangaben: | Moritz Hacke, Charlotte Kaplan, Vibor Laketa, Mike Heilemann, Barbara Müller, and Hans-Georg Kräusslich |
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| 520 | |a Resolving the nanoscale organization of viral and host proteins is important to understanding virion assembly and infectivity. Here, we present a robust framework for multiplexed optical 3D super-resolution microscopy of human immunodeficiency virus type 1 (HIV-1) particles using minimal fluorescence photon flux (MINFLUX) nanoscopy and DNA point accumulation for imaging in nanoscale topography (DNA-PAINT), achieving isotropic localization precision below 10 nm for five target proteins. First, we assessed linkage errors introduced by different labeling strategies by employing the HIV-1 matrix layer as a reference structure. We then extended the approach to display five viral and host proteins and mapped the spatial organization of tetraspanin proteins CD9 and CD81 in single virus-like particles. For accurate visualization and quantitation of multicolor 3D MINFLUX imaging data, we developed the analysis workflow and software matFLUX. The approach presented here enables high-resolution spatial mapping of protein components within individual virus particles and is generally applicable to the study of nanoscale architectures in 3D. | ||
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