From sample to mixed reality: a translational 3D MALDI imaging platform for advanced 3D spatial omics analysis of 3D cell culture disease models
Human 3D cell cultures, including spheroids and organoids, are essential biological models for translational pharmaceutical and biomedical research. However, their 3D analysis using 2D- matrix-assisted laser desorption/ionization (MALDI) imaging-based spatial metabolomics remains challenging, since...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2026
|
| In: |
Advanced science
Year: 2026, Pages: 1-13 |
| ISSN: | 2198-3844 |
| DOI: | 10.1002/advs.202516098 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1002/advs.202516098 Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202516098 
|
| Verfasserangaben: | Stefania Alexandra Iakab, Jonas Cordes, Thomas Enzlein, Florian Keller, Kevin Kastner, Theresa Mulholland, Björn Christian Fröhlich, Lars Gruber, James Lucas Cairns, Stefan Schmidt, Mathias Hafner, Richard Schneider, Johannes Betge, Frank Fischer, Julian Reichwald, Rüdiger Rudolf, and Carsten Hopf |
MARC
| LEADER | 00000naa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1960508229 | ||
| 003 | DE-627 | ||
| 005 | 20260210124014.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 260210s2026 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/advs.202516098 |2 doi | |
| 035 | |a (DE-627)1960508229 | ||
| 035 | |a (DE-599)KXP1960508229 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Iakab, Stefania Alexandra |e VerfasserIn |0 (DE-588)1373121726 |0 (DE-627)1932424393 |4 aut | |
| 245 | 1 | 0 | |a From sample to mixed reality |b a translational 3D MALDI imaging platform for advanced 3D spatial omics analysis of 3D cell culture disease models |c Stefania Alexandra Iakab, Jonas Cordes, Thomas Enzlein, Florian Keller, Kevin Kastner, Theresa Mulholland, Björn Christian Fröhlich, Lars Gruber, James Lucas Cairns, Stefan Schmidt, Mathias Hafner, Richard Schneider, Johannes Betge, Frank Fischer, Julian Reichwald, Rüdiger Rudolf, and Carsten Hopf |
| 246 | 3 | 0 | |a three |
| 264 | 1 | |c 2026 | |
| 300 | |b Illustrationen | ||
| 300 | |a 13 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Online vorab veröffentlicht: 17. Dezember 2025 | ||
| 500 | |a Gesehen am 10.02.2026 | ||
| 520 | |a Human 3D cell cultures, including spheroids and organoids, are essential biological models for translational pharmaceutical and biomedical research. However, their 3D analysis using 2D- matrix-assisted laser desorption/ionization (MALDI) imaging-based spatial metabolomics remains challenging, since end-to-end solutions for 3D-enabling sample preparation, 3D-data processing, 3D-rendering, and 3D-user interaction are lacking. Here, a 3D-MALDI imaging platform and resource that advances each of three pillars is presented: i) the sample preparation introduces custom-designed molds for precise and reproducible embedding and cryosectioning of multiple spheroids and organoids, a substantial improvement over ad hoc or single-sample sectioning workflows; ii) the integrated computational framework that facilitates the generation of high-fidelity volumetric datasets that enable voxel-based analysis for feature discovery, surpassing traditional slice-based 2D analysis; iii) the mixed reality tool enables immersive spatial exploration of molecular distributions in 3D, extending user engagement beyond static 3D renderings. The versatility of the platform is illustrated by its translation to a clinical framework for the molecular profiling of patient-derived colon cancer organoids. Collectively, this integrated approach enables spatial metabolomic analysis in 3D, offers increased throughput, and paves the way for next-generation molecular diagnostics and personalized medicine applications. | ||
| 650 | 4 | |a 3D reconstruction | |
| 650 | 4 | |a cancer research | |
| 650 | 4 | |a mass spectrometry imaging | |
| 650 | 4 | |a patient-derived organoid | |
| 650 | 4 | |a pharmaceutical R&D | |
| 650 | 4 | |a translational clinical research | |
| 700 | 1 | |a Cordes, Jonas |d 1990- |e VerfasserIn |0 (DE-588)1359584056 |0 (DE-627)1919626387 |4 aut | |
| 700 | 1 | |a Enzlein, Thomas |e VerfasserIn |4 aut | |
| 700 | 1 | |a Keller, Florian |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kastner, Kevin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Mulholland, Theresa |e VerfasserIn |4 aut | |
| 700 | 1 | |a Fröhlich, Björn Christian |e VerfasserIn |4 aut | |
| 700 | 1 | |a Gruber, Lars |e VerfasserIn |0 (DE-588)1381766595 |0 (DE-627)1941384013 |4 aut | |
| 700 | 1 | |a Cairns, James L. |d 1994- |e VerfasserIn |0 (DE-588)1361469080 |0 (DE-627)1920670130 |4 aut | |
| 700 | 1 | |a Schmidt, Stefan |e VerfasserIn |0 (DE-588)1339392119 |0 (DE-627)189907497X |4 aut | |
| 700 | 1 | |a Hafner, Mathias |d 1956- |e VerfasserIn |0 (DE-588)1027781187 |0 (DE-627)729718379 |0 (DE-576)375302956 |4 aut | |
| 700 | 1 | |a Schneider, Richard |e VerfasserIn |4 aut | |
| 700 | 1 | |a Betge, Johannes |d 1986- |e VerfasserIn |0 (DE-588)108257421X |0 (DE-627)847674347 |0 (DE-576)455525021 |4 aut | |
| 700 | 1 | |a Fischer, Frank |e VerfasserIn |4 aut | |
| 700 | 1 | |a Reichwald, Julian |e VerfasserIn |4 aut | |
| 700 | 1 | |a Rudolf, Rüdiger |e VerfasserIn |0 (DE-588)1056067489 |0 (DE-627)793904439 |0 (DE-576)412074834 |4 aut | |
| 700 | 1 | |a Hopf, Carsten |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Advanced science |d Weinheim : Wiley-VCH, 2014 |g (2026), Artikel-ID e16098, Seite 1-13 |h Online-Ressource |w (DE-627)817357777 |w (DE-600)2808093-2 |w (DE-576)425795926 |x 2198-3844 |7 nnas |a From sample to mixed reality a translational 3D MALDI imaging platform for advanced 3D spatial omics analysis of 3D cell culture disease models |
| 773 | 1 | 8 | |g year:2026 |g elocationid:e16098 |g pages:1-13 |g extent:13 |a From sample to mixed reality a translational 3D MALDI imaging platform for advanced 3D spatial omics analysis of 3D cell culture disease models |
| 856 | 4 | 0 | |u https://doi.org/10.1002/advs.202516098 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |7 0 |
| 856 | 4 | 0 | |u https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202516098 |x Verlag |z kostenfrei |3 Volltext |7 0 |
| 951 | |a AR | ||
| 992 | |a 20260210 | ||
| 993 | |a Article | ||
| 994 | |a 2026 | ||
| 998 | |g 1056067489 |a Rudolf, Rüdiger |m 1056067489:Rudolf, Rüdiger |d 60000 |e 60000PR1056067489 |k 0/60000/ |p 16 | ||
| 998 | |g 108257421X |a Betge, Johannes |m 108257421X:Betge, Johannes |d 60000 |e 60000PB108257421X |k 0/60000/ |p 13 | ||
| 998 | |g 1027781187 |a Hafner, Mathias |m 1027781187:Hafner, Mathias |p 11 | ||
| 998 | |g 1339392119 |a Schmidt, Stefan |m 1339392119:Schmidt, Stefan |d 50000 |e 50000PS1339392119 |k 0/50000/ |p 10 | ||
| 998 | |g 1361469080 |a Cairns, James L. |m 1361469080:Cairns, James L. |d 50000 |e 50000PC1361469080 |k 0/50000/ |p 9 | ||
| 998 | |g 1381766595 |a Gruber, Lars |m 1381766595:Gruber, Lars |d 50000 |e 50000PG1381766595 |k 0/50000/ |p 8 | ||
| 998 | |g 1359584056 |a Cordes, Jonas |m 1359584056:Cordes, Jonas |p 2 | ||
| 998 | |g 1373121726 |a Iakab, Stefania |m 1373121726:Iakab, Stefania |d 700000 |d 721000 |e 700000PI1373121726 |e 721000PI1373121726 |k 0/700000/ |k 1/700000/721000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1960508229 |e 4912328650 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"id":{"eki":["1960508229"],"doi":["10.1002/advs.202516098"]},"name":{"displayForm":["Stefania Alexandra Iakab, Jonas Cordes, Thomas Enzlein, Florian Keller, Kevin Kastner, Theresa Mulholland, Björn Christian Fröhlich, Lars Gruber, James Lucas Cairns, Stefan Schmidt, Mathias Hafner, Richard Schneider, Johannes Betge, Frank Fischer, Julian Reichwald, Rüdiger Rudolf, and Carsten Hopf"]},"physDesc":[{"extent":"13 S.","noteIll":"Illustrationen"}],"recId":"1960508229","note":["Online vorab veröffentlicht: 17. Dezember 2025","Gesehen am 10.02.2026"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"language":["eng"],"title":[{"title_sort":"From sample to mixed reality","title":"From sample to mixed reality","subtitle":"a translational 3D MALDI imaging platform for advanced 3D spatial omics analysis of 3D cell culture disease models"}],"relHost":[{"disp":"From sample to mixed reality a translational 3D MALDI imaging platform for advanced 3D spatial omics analysis of 3D cell culture disease modelsAdvanced science","physDesc":[{"extent":"Online-Ressource"}],"recId":"817357777","note":["Gesehen am 21.10.2022"],"type":{"bibl":"periodical","media":"Online-Ressource"},"language":["eng"],"part":{"extent":"13","text":"(2026), Artikel-ID e16098, Seite 1-13","year":"2026","pages":"1-13"},"title":[{"title":"Advanced science","title_sort":"Advanced science"}],"id":{"zdb":["2808093-2"],"doi":["10.1002/(ISSN)2198-3844"],"eki":["817357777"],"issn":["2198-3844"]},"pubHistory":["1.2014 (Dezember)-"],"origin":[{"publisher":"Wiley-VCH","publisherPlace":"Weinheim","dateIssuedKey":"2014","dateIssuedDisp":"2014-"}]}],"person":[{"given":"Stefania Alexandra","role":"aut","family":"Iakab","display":"Iakab, Stefania Alexandra"},{"given":"Jonas","role":"aut","family":"Cordes","display":"Cordes, Jonas"},{"family":"Enzlein","role":"aut","given":"Thomas","display":"Enzlein, Thomas"},{"display":"Keller, Florian","role":"aut","given":"Florian","family":"Keller"},{"family":"Kastner","role":"aut","given":"Kevin","display":"Kastner, Kevin"},{"role":"aut","given":"Theresa","family":"Mulholland","display":"Mulholland, Theresa"},{"display":"Fröhlich, Björn Christian","given":"Björn Christian","role":"aut","family":"Fröhlich"},{"display":"Gruber, Lars","given":"Lars","role":"aut","family":"Gruber"},{"display":"Cairns, James L.","role":"aut","given":"James L.","family":"Cairns"},{"given":"Stefan","role":"aut","family":"Schmidt","display":"Schmidt, Stefan"},{"display":"Hafner, Mathias","family":"Hafner","role":"aut","given":"Mathias"},{"role":"aut","given":"Richard","family":"Schneider","display":"Schneider, Richard"},{"family":"Betge","role":"aut","given":"Johannes","display":"Betge, Johannes"},{"role":"aut","given":"Frank","family":"Fischer","display":"Fischer, Frank"},{"family":"Reichwald","given":"Julian","role":"aut","display":"Reichwald, Julian"},{"display":"Rudolf, Rüdiger","family":"Rudolf","role":"aut","given":"Rüdiger"},{"family":"Hopf","role":"aut","given":"Carsten","display":"Hopf, Carsten"}],"origin":[{"dateIssuedKey":"2026","dateIssuedDisp":"2026"}]} | ||
| SRT | |a IAKABSTEFAFROMSAMPLE2026 | ||