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From sample to mixed reality: a translational 3D MALDI imaging platform for advanced 3D spatial omics analysis of 3D cell culture disease models

Human 3D cell cultures, including spheroids and organoids, are essential biological models for translational pharmaceutical and biomedical research. However, their 3D analysis using 2D- matrix-assisted laser desorption/ionization (MALDI) imaging-based spatial metabolomics remains challenging, since...

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Hauptverfasser: Iakab, Stefania Alexandra (VerfasserIn) , Cordes, Jonas (VerfasserIn) , Enzlein, Thomas (VerfasserIn) , Keller, Florian (VerfasserIn) , Kastner, Kevin (VerfasserIn) , Mulholland, Theresa (VerfasserIn) , Fröhlich, Björn Christian (VerfasserIn) , Gruber, Lars (VerfasserIn) , Cairns, James L. (VerfasserIn) , Schmidt, Stefan (VerfasserIn) , Hafner, Mathias (VerfasserIn) , Schneider, Richard (VerfasserIn) , Betge, Johannes (VerfasserIn) , Fischer, Frank (VerfasserIn) , Reichwald, Julian (VerfasserIn) , Rudolf, Rüdiger (VerfasserIn) , Hopf, Carsten (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2026
In: Advanced science
Year: 2026, Pages: 1-13
ISSN:2198-3844
DOI:10.1002/advs.202516098
Online-Zugang:Verlag, kostenfrei, Volltext: https://doi.org/10.1002/advs.202516098
Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202516098
Volltext
Verfasserangaben:Stefania Alexandra Iakab, Jonas Cordes, Thomas Enzlein, Florian Keller, Kevin Kastner, Theresa Mulholland, Björn Christian Fröhlich, Lars Gruber, James Lucas Cairns, Stefan Schmidt, Mathias Hafner, Richard Schneider, Johannes Betge, Frank Fischer, Julian Reichwald, Rüdiger Rudolf, and Carsten Hopf
Beschreibung
Zusammenfassung:Human 3D cell cultures, including spheroids and organoids, are essential biological models for translational pharmaceutical and biomedical research. However, their 3D analysis using 2D- matrix-assisted laser desorption/ionization (MALDI) imaging-based spatial metabolomics remains challenging, since end-to-end solutions for 3D-enabling sample preparation, 3D-data processing, 3D-rendering, and 3D-user interaction are lacking. Here, a 3D-MALDI imaging platform and resource that advances each of three pillars is presented: i) the sample preparation introduces custom-designed molds for precise and reproducible embedding and cryosectioning of multiple spheroids and organoids, a substantial improvement over ad hoc or single-sample sectioning workflows; ii) the integrated computational framework that facilitates the generation of high-fidelity volumetric datasets that enable voxel-based analysis for feature discovery, surpassing traditional slice-based 2D analysis; iii) the mixed reality tool enables immersive spatial exploration of molecular distributions in 3D, extending user engagement beyond static 3D renderings. The versatility of the platform is illustrated by its translation to a clinical framework for the molecular profiling of patient-derived colon cancer organoids. Collectively, this integrated approach enables spatial metabolomic analysis in 3D, offers increased throughput, and paves the way for next-generation molecular diagnostics and personalized medicine applications.
Beschreibung:Online vorab veröffentlicht: 17. Dezember 2025
Gesehen am 10.02.2026
Beschreibung:Online Resource
ISSN:2198-3844
DOI:10.1002/advs.202516098

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