Adenovirus maturation establishes the transcription competent packaging of its genome
Adenoviruses are human pathogens that more recently have gathered interest as tools for human gene therapy and vaccination. The maturation of the viral genome with associated proteins (core) remains largely unexplored. Here, we show that adenovirus core maturation is guided by features embedded in t...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
21 October 2025
|
| In: |
EMBO reports
Year: 2025, Volume: 26, Issue: 22, Pages: 5589-5611 |
| ISSN: | 1469-3178 |
| DOI: | 10.1038/s44319-025-00598-z |
| Online Access: | Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s44319-025-00598-z Verlag, kostenfrei, Volltext: https://link.springer.com/article/10.1038/s44319-025-00598-z 
|
| Author Notes: | Conradin Baumgartl, Simon Holzinger, Uwe Schwartz, Linda E. Franken, Kay Grünewald, Harald Wodrich & Gernot Längst |
MARC
| LEADER | 00000naa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1960548662 | ||
| 003 | DE-627 | ||
| 005 | 20260210153606.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 260210s2025 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s44319-025-00598-z |2 doi | |
| 035 | |a (DE-627)1960548662 | ||
| 035 | |a (DE-599)KXP1960548662 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Baumgartl, Conradin |d 1994- |e VerfasserIn |0 (DE-588)1275151981 |0 (DE-627)1826718192 |4 aut | |
| 245 | 1 | 0 | |a Adenovirus maturation establishes the transcription competent packaging of its genome |c Conradin Baumgartl, Simon Holzinger, Uwe Schwartz, Linda E. Franken, Kay Grünewald, Harald Wodrich & Gernot Längst |
| 264 | 1 | |c 21 October 2025 | |
| 300 | |b Illustrationen | ||
| 300 | |a 23 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 10.02.2026 | ||
| 520 | |a Adenoviruses are human pathogens that more recently have gathered interest as tools for human gene therapy and vaccination. The maturation of the viral genome with associated proteins (core) remains largely unexplored. Here, we show that adenovirus core maturation is guided by features embedded in the viral DNA sequence, which primes the genome for transcription. Using DMS-seq to compare the accessibility of the nucleoprotein core structure before and after maturation (using the maturation deficient ts1 mutant), we identified five genomic regions that become specifically decompacted during maturation. These regions are characterized by low GC-content and are evolutionarily conserved across different adenovirus species, independent of protein-coding constraints. Adenoviral DNA packaging is guided by a distinct 6.1-bp dinucleotide periodicity pattern that helps position viral chromatin proteins. Core maturation serves a dual purpose: (i) it contributes to capsid uncoating by increasing internal pressure while (ii) simultaneously preparing the viral chromatin structure for rapid transcription upon nuclear entry. These findings reveal how sequence-encoded structural information guides adenoviral genome organization and suggest new approaches for optimizing therapeutical adenoviral vectors. | ||
| 650 | 4 | |a Adenovirus | |
| 650 | 4 | |a Bioinformatics | |
| 650 | 4 | |a DMS-Seq | |
| 650 | 4 | |a Virus Maturation | |
| 700 | 1 | |a Holzinger, Simon |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schwartz, Uwe |e VerfasserIn |4 aut | |
| 700 | 1 | |a Franken, Linda E. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Grünewald, Kay |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wodrich, Harald |e VerfasserIn |4 aut | |
| 700 | 1 | |a Längst, Gernot |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |a European Molecular Biology Organization |t EMBO reports |d [London] : Nature Publishing Group UK, 2000 |g 26(2025), 22, Seite 5589-5611 |h Online-Ressource |w (DE-627)320645622 |w (DE-600)2025376-X |w (DE-576)095092153 |x 1469-3178 |7 nnas |
| 773 | 1 | 8 | |g volume:26 |g year:2025 |g number:22 |g pages:5589-5611 |g extent:23 |a Adenovirus maturation establishes the transcription competent packaging of its genome |
| 856 | 4 | 0 | |u https://doi.org/10.1038/s44319-025-00598-z |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |7 0 |
| 856 | 4 | 0 | |u https://link.springer.com/article/10.1038/s44319-025-00598-z |x Verlag |z kostenfrei |3 Volltext |7 0 |
| 951 | |a AR | ||
| 992 | |a 20260210 | ||
| 993 | |a Article | ||
| 994 | |a 2025 | ||
| 998 | |g 1275151981 |a Baumgartl, Conradin |m 1275151981:Baumgartl, Conradin |d 910000 |d 911700 |e 910000PB1275151981 |e 911700PB1275151981 |k 0/910000/ |k 1/910000/911700/ |p 1 |x j | ||
| 999 | |a KXP-PPN1960548662 |e 4912418862 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 10.02.2026"],"language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"title":[{"title":"Adenovirus maturation establishes the transcription competent packaging of its genome","title_sort":"Adenovirus maturation establishes the transcription competent packaging of its genome"}],"person":[{"role":"aut","given":"Conradin","family":"Baumgartl","display":"Baumgartl, Conradin"},{"display":"Holzinger, Simon","given":"Simon","role":"aut","family":"Holzinger"},{"family":"Schwartz","given":"Uwe","role":"aut","display":"Schwartz, Uwe"},{"family":"Franken","given":"Linda E.","role":"aut","display":"Franken, Linda E."},{"family":"Grünewald","given":"Kay","role":"aut","display":"Grünewald, Kay"},{"family":"Wodrich","role":"aut","given":"Harald","display":"Wodrich, Harald"},{"display":"Längst, Gernot","role":"aut","given":"Gernot","family":"Längst"}],"relHost":[{"disp":"European Molecular Biology OrganizationEMBO reports","physDesc":[{"extent":"Online-Ressource"}],"recId":"320645622","note":["Fortsetzung der Druck-Ausgabe","Gesehen am 11.03.25"],"type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng"],"title":[{"title_sort":"EMBO reports","title":"EMBO reports"}],"origin":[{"publisherPlace":"[London] ; Heidelberg ; Oxford [u.a.] ; London [u.a.] ; Hoboken, NJ [u.a.]","dateIssuedDisp":"[2000]-","publisher":"Nature Publishing Group UK ; EMBO Press ; Oxford Univ. Press ; Nature Publishing Group ; Wiley"}],"part":{"issue":"22","text":"26(2025), 22, Seite 5589-5611","extent":"23","pages":"5589-5611","volume":"26","year":"2025"},"id":{"issn":["1469-3178"],"eki":["320645622"],"zdb":["2025376-X"],"doi":["10.1002/(ISSN)1469-3178"]},"corporate":[{"display":"European Molecular Biology Organization","role":"aut"}],"pubHistory":["1.2000 -"],"name":{"displayForm":["European Molecular Biology Organization"]}}],"origin":[{"dateIssuedDisp":"21 October 2025","dateIssuedKey":"2025"}],"physDesc":[{"extent":"23 S.","noteIll":"Illustrationen"}],"recId":"1960548662","id":{"doi":["10.1038/s44319-025-00598-z"],"eki":["1960548662"]},"name":{"displayForm":["Conradin Baumgartl, Simon Holzinger, Uwe Schwartz, Linda E. Franken, Kay Grünewald, Harald Wodrich & Gernot Längst"]}} | ||
| SRT | |a BAUMGARTLCADENOVIRUS2120 | ||